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Immune Checkpoint Proteins

Immune Checkpoint Proteins
Background

lmmune checkpoint proteins are key regulators of immune response against threats while preserving self-tolerance. The classic immune checkpoint consists of co-inhibitory receptors (CTLA-4, PD-1, TIGIT, LAG-3), which inhibit T-cell function and co-stimulatory receptors (GITR, 4-1BB, OX40, CD40) which stimulate T cell activation. Research on the co-inhibitory receptor CTLA-4 (cytotoxic T-lymphocyte antigen 4) and PD-1 (programmed cell death protein 1) showed significantly improved survival rates of patients with metastatic solid tumors after inhibition of these receptor pathways. Therefore, antibody drugs against immune checkpoints for cancer, tumors and other diseases therapeutics is a key mode in cancer therapy.

Find out how immune checkpoint proteins help your drug development

Pathways of Immune Checkpoint
CD155 ICOSL CD86 CD80 MHC CD40 PD-L2 PD-L1 4-1BBL OX40L GITRL MHC PD-L1 Galectin 9 TIGIT ICOS CTLA-4 CD28 TCR CD40L PD-1 PD-1 4-1BB OX40 GITR TCR PD-1 TIM-3 TCR PD-1 PD-1 LAG-3 MHC PD-L1 PD-L2 MHC-LL Dendritic cell Tumor cell Macrophage T-cells

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Stimulatory signal

Inhibitory signal

Click to learn more immune checkpoint molecules

Product Features & Applications
Product Features:
High purity verified by MALS or HPLCHigh bioactivity verified by ELISA/SPR/FACS, etcHigh batch to batch consistency by strict QC tested
Main Applications:
Product List

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  • Co-inhibitory receptors
  • Co-stimulatory receptors

  • Others
  • Inhibitor screening kits
Molecules Species Tags Conjugates  Features
(Available protocols)
PD-1 Human, Mouse, Canine, Cynomolgus, Rabbit, Rat, Rhesus macaque Fc, His, Fc & His, Fc & Avi & His, Fc & Avi, His & Avi, mFc& Avi, Twin-Strep, Llama Fc , mFc Biotin-labeled, PE-labeled, Unconjugated SDS-PAGE, MALS, HPLC, ELISA, BLI, SPR, FACS
PD-L1 Human, Mouse, Rabbit, Canine, Cynomolgus / Rhesus macaque Fc, His, Fc & Avi, His & Avi, Fc & Avi & His, mFc, LlamaFc, mFc& Avi, Twin-Strep Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA, BLI, SPR, FACS
PD-L2 Human, Mouse Fc, His, Fc & Avi, Avi & His, mFc Biotin-labeled, Unconjugated SDS-PAGE, HPLC, ELISA, SPR, BLI, FACS, Cell based assay
B7-H3 Human, Mouse, Cynomolgus Fc & Avi, Avi & His, His, Fc, Avi & His Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA, Cell based assay
B7-H4 Human, Mouse His, Fc Biotin-labeled, Unconjugated SDS-PAGE, ELISA
B7-H5 Human, Mouse, Rhesus macaque Fc & Avi, Avi & His, His, Fc, mFc Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA, SPR, BLI
BTLA Human, Mouse Fc & Avi, His & Avi, His, mFc, Fc Biotin-labeled, Unconjugated SDS-PAGE, ELISA
CD80(B7-1) Human, Mouse, Cynomolgus / Rhesus macaque Fc, Fc & Avi, Avi & His, mFc, His Biotin-labeled, Unconjugated SDS-PAGE, MALS, HPLC, ELISA, SPR, BLI, FACS
CD86(B7-2) Human, Mouse, Cynomolgus / Rhesus macaque Fc & Avi, Fc, His Biotin-labeled, Unconjugated SDS-PAGE, MALS, HPLC, ELISA, BLI, FACS
CD96 Human His & Avi, His, mFc Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA, BLI, FACS
CD47 Human, Mouse, Rabbit, Rat, Cynomolgus / Rhesus macaque, Canine Fc, His, Fc & Avi, His & Avi, mFc, mFc & Avi, LlamaFc Biotin-labeled, FITC-labeled, Unconjugated SDS-PAGE, MALS, ELISA, SPR, BLI, FACS
CD155 Human, Mouse, Rhesus macaque Fc & Avi, His & Avi, mFc, Fc, His Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA, BLI, FACS
CTLA-4 Human, Mouse, Rabbit, Rat, Cynomolgus / Rhesus macaque Fc, His, Fc & Avi, His & Avi, mFc, mFc& Avi Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA, SPR, BLI, FACS
LAG-3 Human, Mouse, Rat, Cynomolgus, Marmoset Fc, His, Fc & Avi, His & Avi, mFc, mFc & Avi, LlamaFc Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA, SPR, BLI, FACS
TIGIT Human, Mouse, Rabbit, Rat, Cynomolgus / Rhesus macaque, Canine mFc& Avi, Fc & Avi, His & Avi, His, Fc, mFc Biotin-labeled, Unconjugated SDS-PAGE, MALS, HPLC, ELISA, SPR, BLI
TIM-3 Human, Mouse, Cynomolgus Avi & His, mFc, Fc, His Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA, SPR
CD111 Human His, Fc Unconjugated SDS-PAGE, ELISA, BLI
DNAM-1 Human, Cynomolgus Fc & Avi, His, Fc Biotin-labeled, Unconjugated SDS-PAGE, ELISA, BLI, FACS
Galectin-9 Human His, MBP & His Unconjugated SDS-PAGE
HVEM Human His, Twin-Strep, Fc, mFc, Fc & Avi Biotin-labeled, Unconjugated , SDS-PAGE, ELISA, BLI
Nectin-2 Human His, Fc Biotin-labeled, Unconjugated SDS-PAGE, HPLC, ELISA, BLI
Nectin-3 Human His, Fc Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA, BLI
PVRIG Human, Mouse, Cynomolgus Fc & Avi, mFc& Avi, His & Avi, His, mFc Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA, BLI
SIRP alpha Human, Mouse, Cynomolgus Fc, His & Avi, mFc& Avi, mFc, His Biotin-labeled, PE-labeled, Unconjugated SDS-PAGE, MALS, HPLC, ELISA, BLI, FACS
SIRP alpha V2 Human His Biotin-labeled, Unconjugated SDS-PAGE, ELISA
SIRP gamma Human Fc Unconjugated SDS-PAGE, ELISA
CD160 Human His Unconjugated SDS-PAGE, ELISA
Molecules Species Tags Conjugates  Features
(Available protocols)
4-1BB Ligand Human, Mouse, Rat, Cynomolgus Fc, Fc & Avi, mFc, His & Flag Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA, BLI, Cell based assay
4-1BB Human, Mouse, Rat, Rabbit, Cynomolgus / Rhesus macaque, Canine His, Fc, Fc & Avi, His & Avi, mFc Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA, SPR
OX40 Ligand Human, Mouse, Rhesus macaque Fc, His, His & Avi, Avi & Fc, mFc Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA, BLI, FACS
OX40 Human, Mouse, Rabbit, Rat, Rhesus macaque, Marmoset His, Fc, His & Avi, Fc & Avi, Avi & His, mFc Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA, BLI, FACS
ICOS Ligand
(B7-H2)
Human, Mouse, Cynomolgus His, Fc Unconjugated SDS-PAGE, ELISA
ICOS Human, Mouse, Cynomolgus His, Fc, His & Avi Biotin-labeled, Unconjugated SDS-PAGE, ELISA
GITR Ligand Human, Mouse His, Fc, Avi & Fc Biotin-labeled, Unconjugated SDS-PAGE, ELISA
GITR Human, Mouse, Rat, Rhesus macaque, Canine His, Fc, His & Avi, Fc & Avi, mFc Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA
CD48 Human His, Fc, mFc Unconjugated SDS-PAGE, ELISA
HVEM Human His, Fc, Fc & Avi, mFc, TwiStrep Biotin-labeled, Unconjugated SDS-PAGE, MALS, BLI
Nectin-2 Human His, Fc, Fc & Avi, mFc Biotin-labeled, Unconjugated SDS-PAGE, MALS, HPLC, ELISA, BLI
CD40 Ligand Human, Mouse, Human / Rhesus macaque His, Fc, His & Avi, Avi & Fc, Avi & His & Flag, His & Flag, mFc Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA, BLI
CD40 Human, Mouse, Rabbit, Cynomolgus, Rhesus macaque His, Fc, Avi & His, Fc & Avi, mFc Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA, BLI, FACS
CD30 ligand Human His, mFc Unconjugated SDS-PAGE, ELISA, BLI, FACS
CD30 Human His, Fc, Avi & His, LlamaFc Biotin-labeled, PE-labeled, FITC-labeled, Unconjugated SDS-PAGE, MALS, ELISA, BLI, FACS
CD28 Mouse, Human / Cynomolgus / Rhesus macaque His, Fc, His & Avi, Fc & Avi, mFc Biotin-labeled, Unconjugated SDS-PAGE, HPLC, ELISA, SPR, BLI
CD27 Ligand Human, Mouse His & Avi, mFc, His, His & Avi & Flag, His & Flag, Fc Biotin-labeled, FITC-labeled, Unconjugated SDS-PAGE, MALS, HPLC, ELISA
CD27 Human, Mouse His, Fc, Fc & Avi, mFc Biotin-labeled, Unconjugated SDS-PAGE, MALS, HPLC, ELISA
B7-1 Human, Mouse, Cynomolgus / Rhesus macaque Fc, Fc & Avi, Avi & His, mFc, His Biotin-labeled, Unconjugated SDS-PAGE, MALS, HPLC, ELISA, SPR, BLI, FACS
B7-2 Human, Mouse, Cynomolgus / Rhesus macaque His, Fc, Fc & Avi Biotin-labeled, Unconjugated SDS-PAGE, MALS, HPLC, ELISA, BLI, FACS
LIGHT Human His, mFc, Fc Unconjugated SDS-PAGE, MALS, ELISA, BLI
B7-H7 Human, Cynomolgus His, Fc, His & Avi, Fc & Avi Biotin-labeled, Unconjugated SDS-PAGE, MALS, BLI
B7-H6 Human, Cynomolgus His, His & Avi, Fc & Avi Biotin-labeled, Unconjugated SDS-PAGE, ELISA, SPR
2B4 Human His, Fc, Avi & His Biotin-labeled, Unconjugated SDS-PAGE, ELISA
CD28H Human His, Fc Unconjugated SDS-PAGE, ELISA
NKp30 Human His, Fc Unconjugated SDS-PAGE, SPR
Key Features

The protocols are available for free.

Authentic structure verified by SEC-MALS

CD27 Ligand, MALS-validated protein

Figure 1. The purity of Human CD27 Ligand, His,Flag Tag (active trimer) (MALS verified) (Cat. No. CDL-H52Da) is more than 95% in HP-SEC, and around 55-70 kDa verified by SEC-MALS.

High batch to batch consistency

The consistency in purity and bioactivity is essential for recombinant proteins.All our proteins have to pass a variety of purity and bioactivity assessments.

PD-1, MALS-validated protein

Biotinylated Human PD-1 / PDCD1 Protein, Avitag™,His Tag (recommended for biopanning) (MALS verified)(Cat. No. PD1-H82E4

Application 1: Development and screening of antibody drugs

Antibody binding assay

PD-1&PD-L1 pathway

PD-1, MALS-validated protein1

Immobilized Human PD-1, His Tag (Cat. No. PD1-H5221) at 2 μg/mL (100 μL/well) can bind anti-PD-1 mab with a linear range of 0.1-3 ng/mL (Routinely tested).

PD-1&PD-L1 pathway

PD-1, MALS-validated protein2

Serial dilutions of anti-PD-1 mab were added into Human PD-1, Fc Tag, low endotoxin (Cat. No. PD1-H5257): Biotinylated Human PD-L1, Fc, Avitag, His Tag (Cat. No. PD1-H82F3) binding reactions. The half maximal inhibitory concentration (IC50) is 0.5381 μg/mL (Routinely tested).

In addition, ACROBiosystems have also developed PD1: PD-L1 (Cat. No. EP-101) and CD47: SIRP alpha (Cat. No. EP-102) inhibitor screening assay kits that can screen neutralizing antibodies or small molecules with high throughput.

Learn more about inhibitor screening kit

Antibody drug affinity assay

LAG-3 pathway

LAG-3, BLI-validated protein

Anti-LAG-3 MAb (Human IgG1) captured on CM5 chip via Anti-Human IgG Fc antibodies surface, can bind Human LAG-3, His Tag (Cat. No. LA3-H5222) with an affinity constant of 4.57 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

LAG-3 pathway

LAG-3, SPR-validated protein

Loaded Anti-LAG-3 MAb (Human IgG1) on AHC Biosensor, can bind Human LAG-3, His Tag (Cat. No. LA3-H5222) with an affinity constant of 7.47 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Neutralizing antibody binding assay

TIGIT pathway

TIGIT, MALS-validated protein

Serial dilutions of Human TIGIT Neutralizing antibody were added into Human TIGIT, Fc Tag (Cat. No. TIT-H5254): Biotinylated Human CD155, Fc,Avitag (Cat. No. CD5-H82F6) binding reactions. The half maximal inhibitory concentration (IC50) is 0.55834 μg/mL (Routinely tested).

CD47&SIPR α pathway(Cell-based assay)

CD47, FACS-validated protein

FACS analysis shows that the binding of Human CD47 to ACHN expressing SIRP-a was inhibited by increasing concentration of neutralizing SIRP-a antibody. The concentration of Human CD47 used is 20 μg/mL. IC50=9.334 μg/mL

Cell-based assay

4-1BB pathway

4-1BB Ligand, Cell-based assay validated protein

Human 4-1BB Ligand (71-254), Fc Tag, active trimer (Cat. No. 41L-H5265) induce IL-8 secretion in HT1080 human CD137 cell line. The EC50 for this effect is 0.079 μg/mL (Routinely tested).


Pharmacokinetics (PK) analysis

CTLA-4 pathway

CTLA-4 dimer, Cell-based assay validated protein

Immobilized Human CTLA-4, His Tag, active dimer (Cat. No. CT4-H52H9) at 2 μg/mL, add increasing concentrations of CTLA-4 x OX40 Bispecific Antibody in 50% Human serum and then add Biotinylated Human OX40, Avitag, His Tag(Cat. No. TN4-H82E4) at 1 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 4 ng/mL (Intact assay, Routinely tested).

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