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뉴 시리즈 형광 위치 특이적 표기 기술 플랫폼 및 시리즈 제품

CAR 감지를 위한 차세대 도구 Star Staining
배경
Star Staining ACROBiosystems Star Staining 뉴 시리즈 거점 표시기술 플랫폼에 개발한 고품질 형광 라벨링된 단백질입니다. 이 단백질은 배치 간에 우수한 균일성과 높은 일관성이 있고 단백질 활성에 영향을 미치지 않으면서 단백질의 자연적 형태를 유지할수 있습니다. 뛰어난 연구 개발 능력, 뛰어난 기술 플랫폼와 제품,
Star Staining은 CAR-T 세포 품질 관리 및 임상 샘플 분석 및 테스트를 위해 특별히 설계한 것입니다.
유세포 분석는 세포세러피제 개발 과정에서 CAR 양성 T 세포를 검출하는 중요한 기술적 수단이며, 형광표지된 단백질은 플루오레신 그룹으로 미리 표지되어 있기 때문에 원스텝 염색으로 검출을 완료할수 있어 검사가 간단합니다. 검출 프로세스 간소하고 검출 시간을 절약하며, 이차 항체의 사용으로 인한 비특이적 배경을 피함으로써 CAR 표현의 유세포 분석 검출을 위한 첫 번째 선택이 되었습니다.
형광 표시 기술은 고품질 형광 표지 단백질 개발의 핵심입니다.현재 시중에 있는 표시 기술은 전통적인 무작위 화학 표시 방법을 사용함으로, 형광 표지의 균일성을 보장할 수 없고  표지 위치가 단백질의 활성 부위를 덮어 단백질 자체의 활성에 영향을 미칠 수 있음으로  높은 배치 간 일관성과 높은 활성 형광 표지 단백질을 얻기 힘듬니다.연구에 따르면 차세대 형광점 표시기술은 단백질의 특정 부위를 라벨링하는 데 사용할 수 있으며 라벨링 속도는 빠르고 효율적이며 단백질의 자연적 공간 구조에 영향을 미치지 않으며 활성이 좋고 검출 민감도가 높습니다.  제품의 우수한 균일성 및 비특이적인 신호의 없음 이 독특한 점 표시 기술은 고품질 형광 표지 단백질 제품의 개발을 위한 강력한 무기가 되었습니다.
기술원리
전통 화학 표시 기술
형광표시 제품 Star Staining
비선택적 무작위 표지, 단백질의 정상적인 구조에 영향을 미칩니다.
단백질 활성 부위가 덮여 있습니다.
단백질의 친수성이 변경됨으로 단백질이 응집되기 쉽습니다.
제품 배치 간의 차이가 큽니다.
뉴 시리즈 위치 특이적 표기 기술
정점 표지 단백질
단백질 특정 부위 라벨링
표시 부위는 활성 중심에서 멀리 떨어져 있고 단백질의 자연적인 공간 형태를 유지 .
고효율 생체 직교 반응, 비특이적 신호 없음
제품의 균일성이 좋고, 배치 간 높은 일관성
Star Staining 특색

ACROBiosystems는 세포트리몬트 제품 품질관리 및 임상 트리몬트  분석에 적합한 고품질 표준 검사  제품 개발에 매진하고 있으며, 다년간의 축적된 기술 경험을 바탕으로 지속적으로 업데이트 및 완성하고 있습니다. 뛰어난 연구 개발 능력으로 성공적으로 Star Staining 뉴 시리즈 형광점 표시기술 플랫폼을 구축하고 세포 트리몬트  약물의 연구 및 개발 과정을 돕는 Star Staining 형광점 표시 단백질 시리즈 제품를 개발했습니다.

뉴 시리즈 거점 표시 기술을 채용하여 단백질의 고활성을 유지

고민감도 및 특이도, CAR세포 배치 검사로 결과 검증

PBMC 이중염료 배치 검사에 의해 방출되는 매우 낮은 비특이적 신호

높은 균일성 및 배치간 일관성, 높은 수준으로 임상 샘플 분석 및 테스트 요구 사항을 충족함

인기 제품
형광 유형분자제품 번호제품 설명주문/예매
더 많은 star stainning   제품
  • FITC

  • PE

  • APC

  • Alexa Fluor 647

  • Alexa Fluor 555

  • Alexa Fluor 488

Fluorescent LabelsMoleculeProduct Description
FITCBCMAFITC-Labeled Human BCMA / TNFRSF17 Protein, His Tag star staining
CD19FITC-Labeled Human CD19 (20-291) Protein, His Tag star staining
MesothelinFITC-Labeled Human Mesothelin / MSLN (296-580) Protein, His Tag star staining
Glypican 3FITC-Labeled Human Glypican 3 / GPC3 Protein, His Tag star staining
HER2FITC-Labeled Human Her2 / ErbB2 Protein, His Tag star staining
CD7FITC-Labeled Human CD7 Protein, His Tag star staining
Siglec-3FITC-Labeled Human Siglec-3 / CD33 Protein, His Tag star staining
CD30FITC-Labeled Human CD30 / TNFRSF8 Protein, His Tag star staining
EGF RFITC-Labeled Human EGF R Protein, His Tag star staining
EGFRVIIIFITC-Labeled Human EGFRvIII Protein, His Tag star staining
GUCY2CFITC-Labeled Human GUCY2C / Guanylyl cyclase C Protein, His Tag star staining
NKG2DFITC-Labeled Human NKG2D / CD314 Protein, His Tag star staining
FAPFITC-Labeled Human FAP Protein, His Tag star staining
Nectin-4FITC-Labeled Human Nectin-4 Protein, His Tag star staining
CD37FITC-Labeled Human CD37 Protein, His Tag star staining
protein LFITC-Labeled Recombinant Protein L Protein, His Tag star staining
Fluorescent LabelsMoleculeProduct Description
PEBCMAPE-Labeled Human BCMA / TNFRSF17 Protein, His Tag star staining
CD19PE-Labeled Human CD19 (20-291) Protein, His Tag star staining
MesothelinPE-Labeled Human Mesothelin / MSLN (296-580) Protein, His Tag star staining
Glypican 3PE-Labeled Human Glypican 3 / GPC3 Protein, His Tag star staining
NKG2DPE-Labeled Human NKG2D / CD314 Protein, His Tag star staining
FAPPE-Labeled Human FAP Protein, His Tag star staining
Nectin-4PE-Labeled Human Nectin-4 Protein, His Tag star staining
CD37PE-Labeled Human CD37 Protein,  His Tag star staining
protein LPE-Labeled Recombinant Protein L Protein, His Tag star staining
EMMPRINPE-Labeled Human EMMPRIN / CD147 Protein, His Tag star staining
CD300ePE-Labeled Human CD300e Protein, His Tag star staining
HER2PE-Labeled Human Her2 / ErbB2 Protein, His Tag star staining
Siglec-2PE-Labeled Human Siglec-2 / CD22 Protein, His Tag star staining
Siglec-3PE-Labeled Human Siglec-3 / CD33 Protein, His Tag star staining
PD-1PE-Labeled Human PD-1 Protein, His Tag star staining
OX40PE-Labeled Human OX40 Protein, His Tag star staining
Mucin-1PE-Labeled Human MUC-1 Protein, His Tag star staining
TSLP RPE-Labeled Human TSLP R Protein, His Tag star staining
Fluorescent LabelsMoleculeProduct Description
APCBCMAAPC-Labeled Human BCMA / TNFRSF17 Protein, His Tag star staining
CD19APC-Labeled Human CD19 (20-291) Protein, His Tag star staining
MesothelinAPC-Labeled Human Mesothelin / MSLN (296-580) Protein, His Tag star staining
Glypican 3APC-Labeled Human Glypican 3 / GPC3 Protein, His Tag star staining
EMMPRINAPC-Labeled Human EMMPRIN / CD147 Protein, His Tag star staining
CD300eAPC-Labeled Human CD300e Protein, His Tag star staining
HER2APC-Labeled Human Her2 / ErbB2 Protein, His Tag star staining
Siglec-2APC-Labeled Human Siglec-2 / CD22 Protein, His Tag star staining
Siglec-3APC-Labeled Human Siglec-3 / CD33 Protein, His Tag star staining
PD-1APC-Labeled Human PD-1 Protein, His Tag star staining
OX40APC-Labeled Human OX40 Protein, His Tag star staining
Mucin-1APC-Labeled Human Mucin-1/MUC-1 Protein, His Tag star staining
TSLP RAPC-Labeled Human TSLP R Protein, His Tag star staining
Fluorescent LabelsMoleculeProduct Description
Alexa Fluor 647BCMAAlexa Fluor 647-Labeled Human BCMA / TNFRSF17 Protein, His Tag star staining
CD19Alexa Fluor 647-Labeled Human CD19 (20-291) Protein, His Tag star staining
MesothelinAlexa Fluor 647-Labeled Human Mesothelin / MSLN (296-580) Protein, His Tag star staining
Glypican 3Alexa Fluor 647-Labeled Human Glypican 3 / GPC3 Protein, His Tag star staining
EMMPRINAlexa Fluor 647-Labeled Human EMMPRIN / CD147 Protein, His Tag star staining
CD300eAlexa Fluor 647-Labeled Human CD300e Protein, His Tag star staining
HER2Alexa Fluor 647-Labeled Human Her2 / ErbB2 Protein, His Tag star staining
Siglec-2Alexa Fluor 647-Labeled Human Siglec-2 / CD22 Protein, His Tag star staining
Siglec-3Alexa Fluor 647-Labeled Human Siglec-3 / CD33 Protein, His Tag star staining
PD-1Alexa Fluor 647-Labeled Human PD-1 Protein, His Tag star staining
OX40Alexa Fluor 647-Labeled Human OX40 Protein, His Tag star staining
Mucin-1Alexa Fluor 647-Labeled Human Mucin-1/MUC-1 Protein, His Tag star staining
TSLP RAlexa Fluor 647-Labeled Human TSLP R Protein, His Tag star staining
Fluorescent LabelsMoleculeProduct Description
Alexa Fluor 555BCMAAlexa Fluor 555-Labeled Human BCMA / TNFRSF17 Protein, His Tag star staining
CD19Alexa Fluor 555-Labeled Human CD19 (20-291) Protein, His Tag star staining
MesothelinAlexa Fluor 555-Labeled Human Mesothelin / MSLN (296-580) Protein, His Tag star staining
Glypican 3Alexa Fluor 555-Labeled Human Glypican 3 / GPC3 Protein, His Tag star staining
NKG2DAlexa Fluor 555-Labeled Human NKG2D / CD314 Protein, His Tag star staining
FAPAlexa Fluor 555-Labeled Human FAP Protein, His Tag star staining
Nectin-4Alexa Fluor 555-Labeled Human Nectin-4 Protein, His Tag star staining
CD37Alexa Fluor 555-Labeled Human CD37 Protein,  His Tag star staining
protein LAlexa Fluor 555-Labeled Recombinant Protein L Protein, His Tag star staining
Fluorescent LabelsMoleculeProduct Description
Alexa Fluor 488BCMAAlexa Fluor 488-Labeled Human BCMA / TNFRSF17 Protein, His Tag star staining
CD19Alexa Fluor 488-Labeled Human CD19 (20-291) Protein, His Tag star staining
MesothelinAlexa Fluor 488-Labeled Human Mesothelin / MSLN (296-580) Protein, His Tag star staining
Glypican 3Alexa Fluor 488-Labeled Human Glypican 3 / GPC3 Protein, His Tag star staining
EMMPRINAlexa Fluor 488-Labeled Human EMMPRIN / CD147 Protein, His Tag star staining
CD300eAlexa Fluor 488-Labeled Human CD300e Protein, His Tag star staining
HER2Alexa Fluor 488-Labeled Human Her2 / ErbB2 Protein, His Tag star staining
Siglec-2Alexa Fluor 488-Labeled Human Siglec-2 / CD22 Protein, His Tag star staining
Siglec-3Alexa Fluor 488-Labeled Human Siglec-3 / CD33 Protein, His Tag star staining
PD-1Alexa Fluor 488-Labeled Human PD-1 Protein, His Tag star staining
OX40Alexa Fluor 488-Labeled Human OX40 Protein, His Tag star staining
Mucin-1Alexa Fluor 488-Labeled Human Mucin-1/MUC-1 Protein, His Tag star staining
TSLP RAlexa Fluor 488-Labeled Human TSLP R Protein, His Tag star staining
* Some products are still under development. If you have any requirements, please click here to contact us.
데이터 확인

고순도

90%의 Star Staining FITC 표지한 인간 BCMA보다 순도가 높습니다
BCMA SDS-PAGE

FITC-Labeled Human BCMA, His Tag (Cat. No. BCA-HF2H3) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

BCMA SEC-MALS

The purity of FITC-Labeled Human BCMA, His Tag (Cat. No. BCA-HF2H3) is more than 90% and the molecular weight of this protein is around 24-34 kDa verified by SEC-MALS.

높은 생물학적 활성

경품보다 높은 결합 활성
FACS 검증된 BCMA 결합 활성
FACS  인증된 CD19 결합 활성

Binding activity of FITC-Labeled Human BCMA and CD19 protein from two different vendors were evaluated by the FACS analysis. The result showed that ACRO's Star Staining FITC-Labeled Human BCMA (Cat. No. BCA-HF2H3) and CD19 (Cat. No. CD9-HF2H3) protein have a much higher binding activity than that of the other competitor.

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Alexa Fluor 647-Labeled Human Mesothelin (296-580)

The activity of Alexa Fluor 647-Labeled Human Mesothelin (296-580), His Tag (Cat. No. MSN-HA2H5) was higher than other Competitor.


Alexa Fluor 488-Labeled Human Mesothelin / MSLN (296-580) Protein

The activity of Alexa Fluor 488-Labeled Human Mesothelin (296-580), His Tag (Cat. No. MSN-HA2H9) was higher than other Competitor.

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Star Staining PE-CD19 또는 APC-CD19 단백질은 유세포 분석에 의해 고감도로 CD19 CAR 발현을  검출합니다
FACS  인증된 CD19 결합 활성

Anti-CD19 CAR-293 cells were stained with PE-Labeled Human CD19 (20-291) Protein (Cat. No. CD9-HP2H5), His Tag star staining and negative control protein respectively, PE signal was used to evaluate the binding activity.

FACS 검증된 PE-CD19 결합 활성

Anti-CD19 CAR-293 cells were stained with APC-Labeled Human CD19 (20-291) Protein (Cat. No. CD9-HA2H9), His Tag star staining and negative control protein respectively, APC signal was used to evaluate the binding activity.

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형질도입되지 않은 PBMC에 대한 비특이적 결합

Star Staining 제품은 형질도입되지 않은  PBMC에 비특이적으로 결합하지 않고 타사 대비 더 높은 결합 활성을 가지고 있습니다
FACS 검증한 BCMA-PBMC

Non-specific binding to non-transduced PBMCs between PE-Labeled Human BCMA Protein of Acro and competitor. 5e5 of non-transduced PBMCs were stained with PE -Labeled Human BCMA Protein and anti-CD3 antibody, washed and then analyzed with FACS. FITC signal was used to evaluate the expression of CD3+ T cells in non-transduced PBMCs, and PE signal was used to evaluate the non-specific binding activity to non-transduced PBMCs.

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BCMA PBMC 비교 데이터

Non-specific binding to non-transduced PBMCs between FITC-Labeled Human BCMA Protein of Acro and competitor. 5e5 of non-transduced PBMCs were stained with FITC-Labeled Human BCMA Protein and anti-CD3 antibody, washed and then analyzed with FACS. PE signal was used to evaluate the expression of CD3+ T cells in non-transduced PBMCs, and FITC signal was used to evaluate the non-specific binding activity to non-transduced PBMCs.

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자연적인 생물학적 활동을 유지합니다

FACS 및 SPR에 의해 결합 전후의 높은 결합기능을 검증됩니다
BCMA 생체 활성 FACS 검증됨

Binding activity of the Human BCMA before and after FITC labeling was evaluated in the above FACS analysis. The result shows that FITC-Labeled BCMA (Cat. No. BCA-HF2H3) and unconjugated Human BCMA have almost the same level of binding activity.

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BCMA 결합 친화성 SPR로 검증됨
BCMA 결합 친화성 SPR로 검증됨

Binding affinity of the Human BCMA before and after FITC labeling was evaluated in the above SPR analysis (Biacore T200). The result shows that FITC-Labeled (Cat. No. BCA-HF2H3) and unconjugated Human BCMA, His Tag have almost the same level of affinity.

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높은 배치 간 일관성

FACS는 FITC/Alexa Fluor-555/PE 표지 인간 BCMA 단백질의 다양한 배치의 결합 활성을 검증했습니다.
BCMA 결합 친화성 SPR로 검증됨
FITC-BCMA 배치 간 일관성FACS로 검증됨
PE-BCMA 배치 간 일관성 FACS에서 검증됨

Binding activity of different lots of FITC-Labeled Human BCMA (Cat. No. BCA-HF2H3) , AF555-Labeled Human BCMA (Cat. No. BCA-HA2H6) and PE-Labeled Human BCMA (Cat. No. BCA-HP2H7) against anti-BCMA CAR-293 cells was evaluated by flow cytometry. The result shows very high batch-to-batch consistency.

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높은 안정성

PE-BCMA 안정성 FACS에서 검증됨
FITC-BCMA 안정성 FACS에서 검증됨

PE-Labeled Human BCMA (Cat. No. BCA-HP2H7) and FITC-Labeled Human BCMA (Cat. No. BCA-HF2H3)  25℃ for 48 hours and freeze-throw cycles are stable without performance reduction.

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CAR-T 관련 콘텐츠 더 보기

참고 문헌

[1] Abbasov ME, Kavanagh ME, Ichu TA, et al. A proteome-wide atlas of lysine-reactive chemistry [published online ahead of print, 2021 Sep 9]. Nat Chem. 2021;10.1038/s41557-021-00765-4.

[2] Xu L, Kuan SL, Weil T. Contemporary Approaches for Site-Selective Dual Functionalization of Proteins. Angew Chem Int Ed Engl. 2021;60(25):13757-13777.

[3] Devabhaktuni A, Lin S, Zhang L, et al. TagGraph reveals vast protein modification landscapes from large tandem mass spectrometry datasets. Nat Biotechnol. 2019;37(4):469-479.

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