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Fc 수용체 단백질

Fc receptors-High purity
소개

트리몬트용 항체의 효능은 Fab 단편 및 표적 항원에 대한 결합 활성에 따라 달라질 뿐만 아니라 Fc 단편 및 주요 Fc 수용체와의 상호작용에 따라 달라집니다. FcRn(FCGRT&B2M)에 대한 Fc 단편의 결합 친화도는 항체의 반감기를 예측할 것이고, Fc 단편과 FcγRIIIa(CD16a) 간의 결합 친화도는 항체가  ADCC(항체 의존적 세포 매개 세포독성)를 유발하는 능력에 영향이 있습니다. 따라서 후보자는 항체 엔지니어링 중에 수용체 패널에 대해 테스트해야 합니다.

ACROBiosystems는 항체 약물 개발을 촉진하기 위해 공통 변이체를 포함하여 재조합 Fc 수용체 단백질의 포괄적인 컬렉션을 제공합니다.

인기 타깃
                                                 
Fc 수용체 단백질 제품의 장점

HEK293 세포 발현: 번역 후 변형 및 올바른 단백질 폴딩 달성

여러 종: 인간, 마우스, Cynomolgus/Rhesus macaque, 쥐, 돼지, 토끼, 고양이, 소, 다른 종에 완전히 적용될 수 있습니다.

고순도: SDS-PAGE통해  순도>95% 검증, SEC-MALS통해 순도>90% 검증

낮은 내독소:<1.0 EU/µg

고친화성 : SPR&BLI 의해 활동성 보장 하고  무료 실험 프로그램 제공 가능합니다.

AvitagTM 부위 특이적 표지화 비오틴화된 부위 특이적 표지 단백질 제공:  AvitagTM 부위 지향적 표지된 비오틴화된 Fc 수용체 단백질은 높은 표지 효율과 특이적 표지 부위를 가지고 있습니다. 약물 개발 및 공정 최적화 과정에서 streptavidin에 대한 결합을 기반으로 ELISA/SPR/BLI 검출 촉진

SPR 및 BLI에서 확인된 친화도:  활성 보장 및 프로토콜 무료 제공

주요 특징

높은 순도

To meet the high purity requirement of pharmaceutical applications, our production team perform both SDS-PAGE and SEC-MALS analyses to the Fc receptors for QC purposes. It’s noteworthy that it’s essential to use monomeric Fc receptor, which represents its natural state. The formation of oligomers during production may result in artificially enhanced binding interaction due to avidity effect. To avoid such situation, we have established strict internal control standards of monomer purity by SEC-MALS testing. Only those batches meeting all purity requirements are released.

Fc receptors-SPR

                                                   Fig. 1 The purity of Human CD32a (R167), His Tag (Cat. No. CDA-H5221) is more than 95% verified by SDS-PAGE. The purity of Human CD32a (R167), His Tag (Cat. No. CDA-H5221) is more than 90% verified by SEC-MALS. And the molecular weight of this protein is around 25-35 kDa.

SPR&BLI에 의해 검증된 생물 활성

The binding affinity between two molecules can be determined by many methods. However, the studies of Fc interaction are often done by SPR, because traditional ELISA is not appropriate for low affinity receptors such as CD16 and CD32. To assure our Fc receptors deliver expected performance, our QC team use Biacore platform to test our products against reference antibodies. Additionally, to meet more applications in the development of antibody drugs, our QC team also use ForteBio platform to test our products against reference antibodies. All SPR&BLI protocols are available for free.

Fc receptors-SPR

Fig. 2 Human CD64, His Tag (SPR & BLI verified) (Cat. No. FCA-H52H1) captured on CM5 chip via anti-His antibody can bind Herc*ptin with an affinity constant of 4.92 nM as determined in a SPR assay (Biacore 8K) (QC tested).

BLI protocol 신청
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Fc receptors-BLI

Fig. 3 Immobilized Human FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (SPR & BLI verified) (Cat. No.  FCM-H5286) on CM5 Chip via Anti-His antibody, can bind Herc*ptin with an affinity constant of 0.261 μM as determined in a SPR assay (Biacore T200).

SPR protocol 신청
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Fc receptors-BLI

Fig. 4 Loaded Human CD64, His Tag (SPR & BLI verified) (Cat. No. FCA-H52H1) on HIS1K Biosensor, can bind Herc*ptin with an affinity constant of 6.98 nM as determined in BLI assay (ForteBio Octet Red96e).

BLI protocol 신청
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Fc receptors-BLI

Fig. 5 Loaded Human FCGRT&B2M Heterodimer Protein, His Tag (SPR & BLI verified) (Cat. No. FCN-H52W7) on SA Biosensor via Biotin his antibody, can bind Herc*ptin with an affinity constant of 0.14 μM as determined in BLI assay (ForteBio Octet Red96e).

BLI protocol 신청
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높은 배치 간 일관성

We routinely apply rigorous quality control measures to ensure consistent performance of our product. As shown below, the batch variation among the tested samples is negligible.

Fc receptors-High batch to batch consistency

Fig. 6 Immobilized Human Fc gamma RIIB / CD32b Protein (Cat. No. CDB-H5228) on CM5 Chip via anti-His antibody, can bind Ritux*mab with an affinity constant of 10 μM as determined in a SPR assay (Biacore T200).

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We have compared the performance of different batches of CD32b (CDB-H5228) in the same assay, and the software analysis showed that the similarity score was very high, meaning high batch-to-batch consistency.

Fc receptors-High batch to batch consistency

Fig. 7 Batch consistency of Human Fc gamma RIIB / CD32b (Cat. No. CDB-H5228). The consistency of different batches is more than 90%.

바이오틴화 또는 기타 종의 FC 수용체 사용 가능

The use of biotin labeling can make your assay development much easier. We offer a variety of ready-to-use biotinylated Fc receptors. These proteins are produced using our in-house developed labeling techniques, which confers high bioactivity and minimal batch-to-batch variation. In addition, we have developed a series of Fc receptors of other species, which are suitable for the screening of non-humanized antibodies or the species cross reaction.

Fc receptors-High batch to batch consistency

Fig. 8 Biotinylated Human CD16a (V176), His Tag, Avi Tag (Cat. No.CDA-H82E9) captured on Biotin CAP- Series S Sensor Chip can bind Ritux*mab with an affinity constant of 0.261 μM as determined in a SPR assay (Biacore T200).

SPR protocol 신청
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Fc receptors-High batch to batch consistency

Fig. 9 Immobilized Cynomolgus / Rhesus macaque FcRn Protein (Cat. No. FCM-C5284) on CM5 Chip via anti-His antibody, can bind Herc*ptin with an affinity constant of 0.403 μM as determined in a SPR assay (Biacore T200).

SPR protocol 신청
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제품 목록
(모든 Fc 수용체는 HEK293에서 발현됨)
  • FcRn

  • FcγR

  • FcεR

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