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Offering SPR-BLI Services - Proteins provided for free! Offering SPR-BLI Services - Proteins provided for free!
Here come GMP Grade Cytokines!Free Sample is available! Here come GMP Grade Cytokines!Free Sample is available!
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Fc 수용체 단백질
트리몬트용 항체의 효능은 Fab 단편 및 표적 항원에 대한 결합 활성에 따라 달라질 뿐만 아니라 Fc 단편 및 주요 Fc 수용체와의 상호작용에 따라 달라집니다. FcRn(FCGRT&B2M)에 대한 Fc 단편의 결합 친화도는 항체의 반감기를 예측할 것이고, Fc 단편과 FcγRIIIa(CD16a) 간의 결합 친화도는 항체가 ADCC(항체 의존적 세포 매개 세포독성)를 유발하는 능력에 영향이 있습니다. 따라서 후보자는 항체 엔지니어링 중에 수용체 패널에 대해 테스트해야 합니다.
ACROBiosystems는 FcRn, FcγRs 및 그의 일반적인 변이체 포함한 포괄적인 Fc 수용체 제품을 제공해서 항체 약물의 연구 개발을 가속화하도록 돕습니다.
신제품추천: FcRn Binding Kit (TR-FRET)
편리한 사용, 높은 감도, 정확한 결과의 특성을 가지고 있으며 항체 약물의 고처리량 스크리닝에 이상적인 솔루션으로 항체 약물의 연구 개발 과정을 지원하고 가속화할 수 있습니다.
HEK293 세포 발현: 번역 후 변형 및 올바른 단백질 폴딩 달성
여러 종: 인간, 마우스, Cynomolgus/Rhesus macaque, 쥐, 돼지, 토끼, 고양이, 소, 다른 종에 완전히 적용될 수 있습니다.
고순도: SDS-PAGE통해 순도>95% 검증, SEC-MALS통해 순도>90% 검증
낮은 내독소:<1.0 EU/µg
고친화성 : SPR&BLI 의해 활동성 보장 하고 무료 실험 프로그램 제공 가능합니다.
AvitagTM 부위 특이적 표지화 비오틴화된 부위 특이적 표지 단백질 제공: AvitagTM 부위 지향적 표지된 비오틴화된 Fc 수용체 단백질은 높은 표지 효율과 특이적 표지 부위를 가지고 있습니다. 약물 개발 및 공정 최적화 과정에서 streptavidin에 대한 결합을 기반으로 ELISA/SPR/BLI 검출 촉진
SPR 및 BLI에서 확인된 친화도: 활성 보장 및 프로토콜 무료 제공
To meet the high purity requirement of pharmaceutical applications, our production team perform both SDS-PAGE and SEC-MALS analyses to the Fc receptors for QC purposes. It’s noteworthy that it’s essential to use monomeric Fc receptor, which represents its natural state. The formation of oligomers during production may result in artificially enhanced binding interaction due to avidity effect. To avoid such situation, we have established strict internal control standards of monomer purity by SEC-MALS testing. Only those batches meeting all purity requirements are released.
Fig. 1 The purity of Human CD32a (R167), His Tag (Cat. No. CDA-H5221) is more than 95% verified by SDS-PAGE. The purity of Human CD32a (R167), His Tag (Cat. No. CDA-H5221) is more than 90% verified by SEC-MALS. And the molecular weight of this protein is around 25-35 kDa.
The binding affinity between two molecules can be determined by many methods. However, the studies of Fc interaction are often done by SPR, because traditional ELISA is not appropriate for low affinity receptors such as CD16 and CD32. To assure our Fc receptors deliver expected performance, our QC team use Biacore platform to test our products against reference antibodies. Additionally, to meet more applications in the development of antibody drugs, our QC team also use ForteBio platform to test our products against reference antibodies. All SPR&BLI protocols are available for free.
Fig. 2 Human CD64, His Tag (SPR & BLI verified) (Cat. No. FCA-H52H1) captured on CM5 chip via anti-His antibody can bind Herc*ptin with an affinity constant of 4.92 nM as determined in a SPR assay (Biacore 8K) (QC tested).
Fig. 3 Immobilized Human FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (SPR & BLI verified) (Cat. No. FCM-H5286) on CM5 Chip via Anti-His antibody, can bind Herc*ptin with an affinity constant of 0.261 μM as determined in a SPR assay (Biacore T200).
Fig. 4 Loaded Human CD64, His Tag (SPR & BLI verified) (Cat. No. FCA-H52H1) on HIS1K Biosensor, can bind Herc*ptin with an affinity constant of 6.98 nM as determined in BLI assay (ForteBio Octet Red96e).
Fig. 5 Loaded Human FCGRT&B2M Heterodimer Protein, His Tag (SPR & BLI verified) (Cat. No. FCN-H52W7) on SA Biosensor via Biotin his antibody, can bind Herc*ptin with an affinity constant of 0.14 μM as determined in BLI assay (ForteBio Octet Red96e).
We routinely apply rigorous quality control measures to ensure consistent performance of our product. As shown below, the batch variation among the tested samples is negligible.
Fig. 6 Immobilized Human Fc gamma RIIB / CD32b Protein (Cat. No. CDB-H5228) on CM5 Chip via anti-His antibody, can bind Ritux*mab with an affinity constant of 10 μM as determined in a SPR assay (Biacore T200).
We have compared the performance of different batches of CD32b (CDB-H5228) in the same assay, and the software analysis showed that the similarity score was very high, meaning high batch-to-batch consistency.
Fig. 7 Batch consistency of Human Fc gamma RIIB / CD32b (Cat. No. CDB-H5228). The consistency of different batches is more than 90%.
The use of biotin labeling can make your assay development much easier. We offer a variety of ready-to-use biotinylated Fc receptors. These proteins are produced using our in-house developed labeling techniques, which confers high bioactivity and minimal batch-to-batch variation. In addition, we have developed a series of Fc receptors of other species, which are suitable for the screening of non-humanized antibodies or the species cross reaction.
Fig. 8 Biotinylated Human CD16a (V176), His Tag, Avi Tag (Cat. No.CDA-H82E9) captured on Biotin CAP- Series S Sensor Chip can bind Ritux*mab with an affinity constant of 0.261 μM as determined in a SPR assay (Biacore T200).
Fig. 9 Immobilized Cynomolgus / Rhesus macaque FcRn Protein (Cat. No. FCM-C5284) on CM5 Chip via anti-His antibody, can bind Herc*ptin with an affinity constant of 0.403 μM as determined in a SPR assay (Biacore T200).
FcRn
FcγR
FcεR
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Authors: Kielau Kisalu, Neville et al.
Journal: JCI Insight 2020
Cited Product: FCM-H5286,FCM-C5284,FCM-M82W5
Application: BLI
Authors: Asokan M, Dias J, Liu C, et al
Cited Product: FCM-C5284
Journal: Proc Natl Acad Sci U S A2020
Application: BLI
Authors: Simonov V, et al
Cited Product: FCM-H5286
Journal: Biologicals 2019
Application: BLI
Engineered antibody Fc variants for enhanced serum half life
Authors: G Georgiou, et al
Cited Product: FCM-H5286
Journal: US20190048078A1 2019
Application: SPR
Authors: Yang B, et al.
Cited Product: FCM-H5286,FCM-M52W2
Journal: Biomater Sci 2018
Application: ELISA
Authors: Zhao C, et al.
Cited Product: FCM-H5286
Journal: Mol Cell Endocrinol 2018
Application: SPR
Authors: Kenniston JA, et al.
Cited Product: FCM-H5286,FCM-M52W2,FCM-R5287,FCM-C5284
Journal: J Biol Chem 2017
Application: ELISA & SPR
Single chain Fc-dimer-human growth hormone fusion protein for improved drug delivery
Authors: Li Zhou, et al.
Cited Product: FCM-H5286
Journal: Biomaterials 2016
Application: SPR
CD38 ecto-enzyme in immune cells is induced during aging and regulates NAD + and N*N levels
Authors: Chini CCS, Peclat TR, Warner GM, et al.
Cited Product: FC4-M52H3,CD4-M5227
Journal: Nature metabolism 2020
Application: Binding Assay
Authors: Simonov V, et al
Cited Product: CDB-H5228,CDA-H5221,CDA-H5220,CDB-H5222
Journal: Biologicals 2019
Application: SPR
Identification of anti-CD16a single domain antibodies and their application in bispecific antibodies
Authors: Zhao Y, et al
Cited Product: CDA-H5220
Journal: Cancer Biol Ther 2019
Application: immunization
Charge variant analysis of proposed biosimilar to Trastuz*mab
Authors: Dakshinamurthy P, et al.
Cited Product: CD8-H52H4
Journal: Biologicals 2017
Application: SPR
Sialylation of immunoglobulin E is a determinant of allergic pathogenicity
Authors: Kai Ting C Shade, et al
Cited Product: CD3-H5249
Journal: Nature 2020
Application: BLI
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