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면역관문 단백질

면역 관문 단백질
배경

면역 체크포인트는 면역 체계 기능의 핵심 조절자입니다. 암, 종양, 자가면역 질환 및 기타 질병의 처치를 위한 면역관문에 대한 항체 약물의 개발은 면역 요법의 중요한 수단입니다. 현재 가장 많이 연구되고 있는 억제성 면역관문경로는 CTLA-4와 PD-1/PD-L1이며 면역관문을 차단하여 악성종양을 처치하는 연구는 LAG-3, TIM-3, TIGIT, VISTA, B7-H3와 같은 면역공동억제분자에 대한 항체의약품의 개발에 확장 돼였고  OX40, ICOS, GITR, 4-1BB, CD40과 같은 면역공동자극분자에 대한 표적의약품도 연구개발 단계에 있습니다. 따라서 면역 관문 분자 항체 약물의 연구 개발을 효율적으로 수행하는 방법은 여전히 암, 종양 및 기타 질병에 대한 현재 처치 방법의 초점입니다.

면역 관문 단백질이 약물 개발에 어떻게 도움이 되는지 알아보십시오.

면역 체크포인트의 경로
CD155 ICOSL CD86 CD80 MHC CD40 PD-L2 PD-L1 4-1BBL OX40L GITRL MHC PD-L1 Galectin 9 TIGIT ICOS CTLA-4 CD28 TCR CD40L PD-1 PD-1 4-1BB OX40 GITR TCR PD-1 TIM-3 TCR PD-1 PD-1 LAG-3 MHC PD-L1 PD-L2 MHC-LL Dendritic cell Tumor cell Macrophage T-cells

제품 세부 정보를 보려면 분자 이름을 클릭하십시오.

Stimulatory signal

Inhibitory signal

더 많은 면역 관문 단백질 보기

제품 기능 및 응용
제품 특징
MALS/HPLC로 고순도 검증됨 ELISA/SPR/FACS 등으로 높은 생물학적 활성 검증됨엄격한 QC 품질 검사로 배치 간 일관성 확보
주요 적용 장명:
제품 목록

자세한 내용을 보려면 분자 이름을 클릭하십시오.

  • 공동 억제 수용체

  • 공동 자극 수용체

  • 기타 면역 관문 분자 목록

  • 억제제 스크리닝 키트

분자소속태그접합체검증 데이터
(프로토콜 재공 가능)
PD-1Human, Mouse, Canine, Cynomolgus, Rabbit, Rat, Rhesus macaqueFc, His, Fc & His, Fc & Avi & His, Fc & Avi, His & Avi, mFc& Avi, Twin-Strep, Llama Fc , mFcBiotin-labeled, PE-labeled, UnconjugatedSDS-PAGE, MALS, HPLC, ELISA, BLI, SPR, FACS
PD-L1Human, Mouse, Rabbit, Canine, Cynomolgus / Rhesus macaqueFc, His, Fc & Avi, His & Avi, Fc & Avi & His, mFc, LlamaFc, mFc& Avi, Twin-StrepBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, BLI, SPR, FACS
PD-L2Human, MouseFc, His, Fc & Avi, Avi & His, mFcBiotin-labeled, UnconjugatedSDS-PAGE, HPLC, ELISA, SPR, BLI, FACS, Cell based assay
B7-H3Human, Mouse, CynomolgusFc & Avi, Avi & His, His, Fc, Avi & HisBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, Cell based assay
B7-H4Human, MouseHis, FcBiotin-labeled, UnconjugatedSDS-PAGE, ELISA
B7-H5Human, Mouse, Rhesus macaqueFc & Avi, Avi & His, His, Fc, mFcBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, SPR, BLI
BTLAHuman, MouseFc & Avi, His & Avi, His, mFc, FcBiotin-labeled, UnconjugatedSDS-PAGE, ELISA
CD80(B7-1)Human, Mouse, Cynomolgus / Rhesus macaqueFc, Fc & Avi, Avi & His, mFc, HisBiotin-labeled, UnconjugatedSDS-PAGE, MALS, HPLC, ELISA, SPR, BLI, FACS
CD86(B7-2)Human, Mouse, Cynomolgus / Rhesus macaqueFc & Avi, Fc, HisBiotin-labeled, UnconjugatedSDS-PAGE, MALS, HPLC, ELISA, BLI, FACS
CD96HumanHis & Avi, His, mFcBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, BLI, FACS
CD47Human, Mouse, Rabbit, Rat, Cynomolgus / Rhesus macaque, CanineFc, His, Fc & Avi, His & Avi, mFc, mFc & Avi, LlamaFcBiotin-labeled, FITC-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, SPR, BLI, FACS
CD155Human, Mouse, Rhesus macaqueFc & Avi, His & Avi, mFc, Fc, HisBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, BLI, FACS
CTLA-4Human, Mouse, Rabbit, Rat, Cynomolgus / Rhesus macaqueFc, His, Fc & Avi, His & Avi, mFc, mFc& AviBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, SPR, BLI, FACS
LAG-3Human, Mouse, Rat, Cynomolgus, MarmosetFc, His, Fc & Avi, His & Avi, mFc, mFc & Avi, LlamaFcBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, SPR, BLI, FACS
TIGITHuman, Mouse, Rabbit, Rat, Cynomolgus / Rhesus macaque, CaninemFc& Avi, Fc & Avi, His & Avi, His, Fc, mFcBiotin-labeled, UnconjugatedSDS-PAGE, MALS, HPLC, ELISA, SPR, BLI
TIM-3Human, Mouse, CynomolgusAvi & His, mFc, Fc, HisBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, SPR
CD111HumanHis, FcUnconjugatedSDS-PAGE, ELISA, BLI
DNAM-1Human, CynomolgusFc & Avi, His, FcBiotin-labeled, UnconjugatedSDS-PAGE, ELISA, BLI, FACS
Galectin-9HumanHis, MBP & HisUnconjugatedSDS-PAGE
HVEMHumanHis, Twin-Strep, Fc, mFc, Fc & AviBiotin-labeled, Unconjugated ,SDS-PAGE, ELISA, BLI
Nectin-2HumanHis, FcBiotin-labeled, UnconjugatedSDS-PAGE, HPLC, ELISA, BLI
Nectin-3HumanHis, FcBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, BLI
PVRIGHuman, Mouse, CynomolgusFc & Avi, mFc& Avi, His & Avi, His, mFcBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, BLI
SIRP alphaHuman, Mouse, CynomolgusFc, His & Avi, mFc& Avi, mFc, HisBiotin-labeled, PE-labeled, UnconjugatedSDS-PAGE, MALS, HPLC, ELISA, BLI, FACS
SIRP alpha V2HumanHisBiotin-labeled, UnconjugatedSDS-PAGE, ELISA
SIRP gammaHumanFcUnconjugatedSDS-PAGE, ELISA
CD160HumanHisUnconjugatedSDS-PAGE, ELISA
분자소속태그접합체 검증 데이터
(프로토콜 재공 가능)
4-1BB LigandHuman, Mouse, Rat, CynomolgusFc, Fc & Avi, mFc, His & FlagBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, BLI, Cell based assay
4-1BBHuman, Mouse, Rat, Rabbit, Cynomolgus / Rhesus macaque, CanineHis, Fc, Fc & Avi, His & Avi, mFcBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, SPR
OX40 LigandHuman, Mouse, Rhesus macaqueFc, His, His & Avi, Avi & Fc, mFcBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, BLI, FACS
OX40Human, Mouse, Rabbit, Rat, Rhesus macaque, MarmosetHis, Fc, His & Avi, Fc & Avi, Avi & His, mFcBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, BLI, FACS
ICOS Ligand
(B7-H2)
Human, Mouse, CynomolgusHis, FcUnconjugatedSDS-PAGE, ELISA
ICOSHuman, Mouse, CynomolgusHis, Fc, His & AviBiotin-labeled, UnconjugatedSDS-PAGE, ELISA
GITR LigandHuman, MouseHis, Fc, Avi & FcBiotin-labeled, UnconjugatedSDS-PAGE, ELISA
GITRHuman, Mouse, Rat, Rhesus macaque, CanineHis, Fc, His & Avi, Fc & Avi, mFcBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA
CD48HumanHis, Fc, mFcUnconjugatedSDS-PAGE, ELISA
HVEMHumanHis, Fc, Fc & Avi, mFc, TwiStrepBiotin-labeled, UnconjugatedSDS-PAGE, MALS, BLI
Nectin-2HumanHis, Fc, Fc & Avi, mFcBiotin-labeled, UnconjugatedSDS-PAGE, MALS, HPLC, ELISA, BLI
CD40 LigandHuman, Mouse, Human / Rhesus macaqueHis, Fc, His & Avi, Avi & Fc, Avi & His & Flag, His & Flag, mFcBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, BLI
CD40Human, Mouse, Rabbit, Cynomolgus, Rhesus macaqueHis, Fc, Avi & His, Fc & Avi, mFcBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, BLI, FACS
CD30 ligandHumanHis, mFcUnconjugatedSDS-PAGE, ELISA, BLI, FACS
CD30HumanHis, Fc, Avi & His, LlamaFcBiotin-labeled, PE-labeled, FITC-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, BLI, FACS
CD28Mouse, Human / Cynomolgus / Rhesus macaqueHis, Fc, His & Avi, Fc & Avi, mFcBiotin-labeled, UnconjugatedSDS-PAGE, HPLC, ELISA, SPR, BLI
CD27 LigandHuman, MouseHis & Avi, mFc, His, His & Avi & Flag, His & Flag, FcBiotin-labeled, FITC-labeled, UnconjugatedSDS-PAGE, MALS, HPLC, ELISA
CD27Human, MouseHis, Fc, Fc & Avi, mFcBiotin-labeled, UnconjugatedSDS-PAGE, MALS, HPLC, ELISA
B7-1Human, Mouse, Cynomolgus / Rhesus macaqueFc, Fc & Avi, Avi & His, mFc, HisBiotin-labeled, UnconjugatedSDS-PAGE, MALS, HPLC, ELISA, SPR, BLI, FACS
B7-2Human, Mouse, Cynomolgus / Rhesus macaqueHis, Fc, Fc & AviBiotin-labeled, UnconjugatedSDS-PAGE, MALS, HPLC, ELISA, BLI, FACS
LIGHTHumanHis, mFc, FcUnconjugatedSDS-PAGE, MALS, ELISA, BLI
B7-H7Human, CynomolgusHis, Fc, His & Avi, Fc & AviBiotin-labeled, UnconjugatedSDS-PAGE, MALS, BLI
B7-H6Human, CynomolgusHis, His & Avi, Fc & AviBiotin-labeled, UnconjugatedSDS-PAGE, ELISA, SPR
2B4HumanHis, Fc, Avi & HisBiotin-labeled, UnconjugatedSDS-PAGE, ELISA
CD28HHumanHis, FcUnconjugatedSDS-PAGE, ELISA
NKp30HumanHis, FcUnconjugatedSDS-PAGE, SPR
검증 데이터

내부 실험 방법은 참고용으로 무료로 제공받을 수 있으며 제품 상세페 이지에 들어가시면 해당 프로토콜을 신청하실 수 있습니다.

MALS통해 실제 분자량과 폴리머 상태를 확인합니다.

CD27 리간드, MALS 검증 단백질

Figure 1. The purity of Human CD27 Ligand, His,Flag Tag (active trimer) (MALS verified) (Cat. No. CDL-H52Da) is more than 95% in HP-SEC, and around 55-70 kDa verified by SEC-MALS.

높은 배치 간 일관성

The consistency in purity and bioactivity is essential for recombinant proteins.All our proteins have to pass a variety of purity and bioactivity assessments.

PD-1, MALS 검증된 단백질

Biotinylated Human PD-1 / PDCD1 Protein, Avitag™,His Tag (recommended for biopanning) (MALS verified)(Cat. No. PD1-H82E4

면역 및 항체 스크리닝에 적합

항체 바인딩 검증

PD-1&PD-L1 pathway

PD-1, MALS 검증된 단백질1

Immobilized Human PD-1, His Tag (Cat. No. PD1-H5221) at 2 μg/mL (100 μL/well) can bind anti-PD-1 mab with a linear range of 0.1-3 ng/mL (Routinely tested).                  

PD-1&PD-L1 pathway

PD-1, MALS 검증된 단백질2

Serial dilutions of anti-PD-1 mab were added into Human PD-1, Fc Tag, low endotoxin (Cat. No. PD1-H5257): Biotinylated Human PD-L1, Fc, Avitag, His Tag (Cat. No. PD1-H82F3) binding reactions. The half maximal inhibitory concentration (IC50) is 0.5381 μg/mL (Routinely tested).

또한 PD1: PD-L1(제품 번호 EP-101) 및 CD47: SIRP 알파(제품 번호 EP-102) 억제제 스크리닝 분석 키트도 개발했으며 이 키트는 신속하고 높은 처리량으로 억제성 항체 또는 소분자를 스크리닝할 수 있습니다.

클릭하여 더 많은 억제제 스크리닝 키트를 보십시오.

항체 약물 친화도 분석

LAG-3 pathway

LAG-3, BLI 검증된 단백질

Loaded Anti-LAG-3 MAb (Human IgG1) on AHC Biosensor, can bind Human LAG-3, His Tag (Cat. No. LA3-H5222) with an affinity constant of 7.47 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

LAG-3 pathway

LAG-3, SPR 검증 단백질

Anti-LAG-3 MAb (Human IgG1) captured on CM5 chip via Anti-Human IgG Fc antibodies surface, can bind Human LAG-3, His Tag (Cat. No. LA3-H5222) with an affinity constant of 4.57 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

중화 항체 바인딩 분석

TIGIT pathway

TIGIT, MALS 검증 단백질

Serial dilutions of Human TIGIT Neutralizing antibody were added into Human TIGIT, Fc Tag (Cat. No. TIT-H5254): Biotinylated Human CD155, Fc,Avitag (Cat. No. CD5-H82F6) binding reactions. The half maximal inhibitory concentration (IC50) is 0.55834 μg/mL (Routinely tested).

CD47&SIPR α pathway(Cell-based assay)

CD47, FACS 검증 단백질

FACS analysis shows that the binding of Human CD47 to ACHN expressing SIRP-a was inhibited by increasing concentration of neutralizing SIRP-a antibody. The concentration of Human CD47 used is 20 μg/mL. IC50=9.334 μg/mL

세포 기능 검증

4-1BB pathway

4-1BB 리간드, 세포 기반 분석 검증 단백질

Human 4-1BB Ligand (71-254), Fc Tag, active trimer (Cat. No. 41L-H5265) induce IL-8 secretion in HT1080 human CD137 cell line. The EC50 for this effect is 0.079 μg/mL (Routinely tested).


약물동태학(PK) 분석

CTLA-4 pathway

CTLA-4 이량체, 세포 기반 분석 검증 단백질

Immobilized Human CTLA-4, His Tag, active dimer (Cat. No. CT4-H52H9) at 2 μg/mL, add increasing concentrations of CTLA-4 x OX40 Bispecific Antibody in 50% Human serum and then add Biotinylated Human OX40, Avitag, His Tag(Cat. No. TN4-H82E4) at 1 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 4 ng/mL (Intact assay, Routinely tested).

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