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Biotinylated Human PD-1 / PDCD1 Protein, Avitag™,His Tag (recommended for biopanning) (MALS verified)

인기 제품 추천: SP-11 스트렙타비딘 코팅된 96웰 플레이트  샘플 신청 
STN-N5116 스트렙타비딘 단백질  SMB-B01 스트렙타비딘 마그네틱 비드 
비오틴-스트렙타비딘 시스템의 높은 특이성과 고감도를 이용하여 비오틴화된 단백질을 스트렙타비딘 관련 제품과 결합하여 다양한 실험에서 사용할 수 있습니다. 비비오틴 시스템과 비교하면 검출이 더 빠르고 더 강력하고 더 낮은 비특이적 배경을 가지고 있습니다.
  • 동의어
    PDCD1,PD1,CD279,SLEB2
  • 출처
    Biotinylated Human PD-1, Avitag,His Tag (recommended for biopanning) (PD1-H82E4) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Gln 167 (Accession # Q15116-1).
    Predicted N-terminus: Leu 25
  • 분자 표징
    Online(Leu 25 - Gln 167) Q15116-1

    This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.

    The protein has a calculated MW of 18.6 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 어플리케이션
    PD1-H82E4 works best for experiments that test the binding between PD-1 and candidate antibodies, such as biopanning and other relevant assays.
    This product is NOT suitable for testing PD1-PDL1 binding by ELISA. For this type of application, we strongly recommend you to choose PD1-H82F1 as an alternative.
  • 비오틴화
    비오틴화 of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • 내독소
    Less than 1.0 EU per μg by the LAL method.
  • 순도

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • 레시피

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • 재구성

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 보관

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human PD-1, Avitag,His Tag (recommended for biopanning) (Cat. No. PD1-H82E4) SDS-PAGE gel

Biotinylated Human PD-1, Avitag,His Tag (recommended for biopanning) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated Human PD-1, Avitag,His Tag (recommended for biopanning) (Cat. No. ) MALS images

The purity of Biotinylated Human PD-1, Avitag,His Tag (recommended for biopanning) (Cat. No. PD1-H82E4) is more than 90% and the molecular weight of this protein is around 32-45 kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated Human PD-1, Avitag,His Tag (recommended for biopanning)Biotinylated Human PD-1, Avitag,His Tag (recommended for biopanning) (Cat. No. PD1-H82E4) ELISA bioactivity

Immobilized Nivolumab at 2 μg/mL (100 μL/well) can bind Biotinylated Human PD-1, Avitag,His Tag (recommended for biopanning) (Cat. No. PD1-H82E4) with a linear range of 0.8-13 ng/mL (QC tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Biotinylated Human PD-1, Avitag,His Tag (recommended for biopanning) (Cat. No. PD1-H82E4) on SA Biosensor, can bind Human PD-L1, His Tag (HPLC verified) (Cat. No. PD1-H5229) with an affinity constant of 2.4 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Human_FcRn_Heterodimer_Protein_Bli

Loaded Biotinylated Human PD-1, Avitag,His Tag (recommended for biopanning) (Cat. No. PD1-H82E4) on SA Biosensor, can bind Human PD-L2, His Tag (SPR verified) (Cat. No. PD2-H5220) with an affinity constant of 0.6 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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