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> 사용자 고유의 자성입자 기반 화학발광면역분석법(Magnetic Particle Chemiluminescent Immunoassay, MPCLIA)을 구축해주는 시약
자성입자(Magnetic Particle, MP) 기반 화학발광면역분석법(Chemiluminescent Immunoassay, CLIA)은 자기 비드의 유연성과 CLIA의 민감도 및 특이도를 결합한 분석 방법입니다. 웰 플레이트 안에 단백질이나 항체를 고정하지 않는 대신 자기 비드는 검체 접촉 표면적, 포착 효율성 및 자동화 기능을 유의하게 향상합니다. 따라서 MPCLIA는 현재 질병 진단, 화학 반응 모니터링, 그 외 여러 연구 분야에서 자주 활용되고 있습니다.
ELISA와 비교할 때 MPCLIA가 갖는 가장 큰 차이점은 자기 비드를 기본 골격으로 사용하는 것 외에도 화학발광을 통해 검출한다는 점입니다. 화학 반응을 활용하여 홍당무과산화효소(horseradish peroxidase, HRP) 및 아크리디늄 에스터(acridinium ester, AE)와 같은 물질을 자극하면 광자가 방출되며 전용 화학발광 판독기로 이를 측정할 수 있습니다. 기존 ELISA와 비교할 때 CLIA 방법은 더 높은 민감도, 특이도, 선형 범위를 갖는 경우가 많습니다.
Chemiluminescence Detection Mechanism
Colorimetric (ELISA) Detection Mechanism
| 제품 유형 | 카탈로그 번호 | 제품 설명 | 사전주문/주문 |
|---|
| 분석 번호 | 자기 비드 | 검체 1 | 검체 2 | 마커 | 기질 솔루션 |
|---|---|---|---|---|---|
| 1 | Protein A-coupled Magnetic Beads (Cat. No. MPC-A001) | Human Antibody | Biotinylated non-Fc tag protein | Streptavidin-Acridine ester (Cat. No. STN-NA114) | Substrate Solution(AE Marker) (Cat. No. ABK-001) |
| 2 | hFc tag protein | ||||
| 3 | Protein G-coupled Magnetic Beads (Cat. No. MPC-A002) | Human/Mouse Antibody | Biotinylated non-Fc tag protein | ||
| 4 | Fc tag protein | ||||
| 5 | Anti-His-coupled Magnetic Beads (Cat. No. MPC-A005) | His tag protein | Biotinylated non-His tag protein | ||
| 6 | Human antibody | Anti-Human IgG-Acridine ester (Cat. No. AHG-Y69) | |||
| 7 | hFc tag protein | ||||
| 8 | Anti-Mouse IgG-coupled Magnetic Beads (Cat. No. MPC-A003) | Mouse antibody | Biotinylated non-mFc tag protein | Streptavidin-Acridine ester (Cat. No. STN-NA114) | |
| 9 | mFc tag protein | ||||
| 10* | Mouse antibody | hFc tag protein | Anti-Human IgG-Acridine ester (Cat. No. AHG-Y69) | ||
| 11* | mFc tag protein | Human antibody | |||
| 12* | hFc tag protein | ||||
| 13 | Streptavidin-Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A006) | Biotinylated non-hFc tag protein | Human antibody | ||
| 14 | hFc tag protein | ||||
| 15 | Biotinylated non-mFc tag protein | Mouse antibody | Anti-Mouse IgG-Acridine ester (Cat. No. AMG-S163) | ||
| 16 | mFc tag protein | ||||
| 17 | Anti-Human IgG-coupled Magnetic Beads (Cat. No. MPC-A004) | Human antibody | mFc tag protein | ||
| 18 | hFc tag protein | ||||
| 19* | hFc tag protein | Mouse antibody | |||
| 20* | Human antibody | Biotinylated non-hFc tag protein | Streptavidin-Acridine ester (Cat. No. STN-NA114) | ||
| 21* | hFc tag protein |
Immobilized 0.04 μg /Test of Biotinylated Human PD-1, Avitag,His Tag (recommended for biopanning) (Cat. No. PD1-H82E4) to the Streptavidin-Acridine ester (Cat. No. STN-NA114, 0.008 μg /Test), incubated with 100 μL /Test of Human PD-L1, Fc Tag (Cat. No. PD1-H5258) at increasing concentration coupled to Protein A-coupled Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A001) (10 μg beads/Test). Detection was performed with sensitivity of 3.125 ng/mL in Magnetic Particle Chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (QC tested).
| Cat. No. | Product Description |
|---|---|
| PD1-H82E4 | Biotinylated Human PD-1 / PDCD1 Protein, Avitag™,His Tag (recommended for biopanning) (MALS verified) |
| STN-NA114 | Streptavidin Protein-Acridine ester (MALS verified) |
| PD1-H5258 | Human PD-L1 / B7-H1 Protein, Fc Tag (MALS verified) |
| MPC-A001 | Protein A-coupled Magnetic Beads (recommended for MPCLIA) |
Immobilized 0.04 μg /Test of Biotinylated Human PD-1, Avitag,His Tag (recommended for biopanning) (Cat. No. PD1-H82E4) to the Streptavidin-Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A006, 20 μg beads/Test), incubated with 100 μL /Test of Human PD-L1, Mouse IgG1 Fc Tag, low endotoxin (Cat. No. PD1-H52A3) at increasing concentration coupled to Anti-Mouse IgG-Acridine ester (Cat. No. AMG-S163, 0.04 μg /Test). Detection was performed with sensitivity of 1.5625 ng/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (QC tested).
| Cat. No. | Product Description |
|---|---|
| PD1-H82E4 | Biotinylated Human PD-1 / PDCD1 Protein, Avitag™,His Tag (recommended for biopanning) (MALS verified) |
| MPC-A006 | Streptavidin-Magnetic Beads (recommended for MPCLIA) |
| PD1-H52A3 | Human PD-L1 / B7-H1 Protein, Mouse IgG1 Fc Tag, low endotoxin (MALS verified) |
| AMG-S163 | Anti-Mouse IgG-Acridine ester (MALS verified) |
Immobilized 0.04 μg /Test of Biotinylated Human PD-1 Protein, Avitag,His Tag (Cat. No. PD1-H82E4) to the Streptavidin-Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A006, 20 μg beads/Test), incubated with 100 μL /Test of Human PD-L1, Fc Tag (Cat. No. PD1-H5258) at increasing concentration coupled to Anti-Human IgG Antibody-Acridine ester (Cat. No. AHG-Y69, 0.04 μg /Test). Detection was performed with sensitivity of 0.488 ng/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (QC tested).
When used the Chemiluminescent Substrate Solution (AE Marker) (Cat. No. ABK-001) in a sandwich MPCLIA Assay for detection of SARS-CoV-2, The Biotinylated Anti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (Cat. No. NUN-BM266) coupled Streptavidin-Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A006) was incubated with Acridine Ester-Labeled Anti-SARS-CoV-2 Nucleocapsid Antibody, Chimeric mAb, Human IgG1 (AM224) (Cat. No. NUN-M224) and treated with SARS-CoV-2 Nucleocapsid protein, His Tag (Cat. No. NUN-C5227) at increasing concentration, detection was performed using with sensitivity of 97.7 pg/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (QC tested).
When used the Chemiluminescent Substrate Solution (HRP Marker) (Cat. No. ABK-002) in a sandwich CLEIA Assay for detection of Human IFN-gamma, immobilized Monoclonal Anti-IFNγ antibody, Human IgG1 at 2 μg/mL, add increasing concentrations of Human IFN-gamma, premium grade (Cat. No. IFG-H4211) and then add Biotinylated Biotinylated Monoclonal Anti-IFNγ antibody, Human IgG at 0.05 μg/mL. Detection was performed using Streptavidin-HRP, Horseradish peroxidase conjugated Streptavidin (Cat. No. STN-NH913) with sensitivity of 7.8 pg/mL.
Immobilized 0.04 μg /Test of Biotinylated Human PD-1, Avitag,His Tag (recommended for biopanning) (Cat. No. PD1-H82E4) to the Streptavidin-Acridine ester (Cat. No. STN-NA114, 0.008 μg /Test), incubated with 100 μL /Test of Human PD-L1, Fc Tag (Cat. No. PD1-H5258) at increasing concentration coupled to Protein A-coupled Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A001) (10 μg beads/Test). Detection was performed with sensitivity of 3.125 ng/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (QC tested).
The MPCLIA assay shows that Protein G-coupled Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A002) is stable at 37°C for 10 days.
The MPCLIA assay shows that Anti-Human IgG-coupled Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A004) is stable after freezing and thawing 3 times.
The MPCLIA assay shows that Anti-His Tag-coupled Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A005) is stable at 37°C for 10 days.
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