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> FITC & PE labeled Proteins
Fluorescent-labeled proteins can be powerful tools for the detection of CAR expression and antibody screening through flow cytometry. However, the development of high-quality fluorescent-labeled proteins presents a major challenge and there are few products commercially available so far.
Positioned as a reliable partner to provide antigens for biologic drug discovery and development, ACROBiosystems has developed an exclusive collection of FITC-labeled and PE-labeled proteins by using our in-house developed chemical labeling technique. These fluorescent-labeled proteins are uniquely suitable for flow cytometry-based assays, such as detection of anti-CD19 CAR expression and cell-based ligand binding assays.
Bioactivity validated by flow cytometry and protocol offered
Identical binding capacity before and after conjugation
High batch-to-batch consistency
FACS Analysis of Anti-CD19 CAR Expression
293 cells were transfected with anti-CD19-scFv and RFP tag. 2e5 of the cells were stained with FITC-Labeled Human CD19 (20-291), Fc Tag (Cat. No. CD9-HF251, 10 μg/ml) and FITC-labeled protein control. Non-transfected 293 cells and FITC-labeled protein control were used as negative control. RFP was used to evaluate CAR (anti-CD19-scFv) expression and FITC was used to evaluate the binding activity of FITC-Labeled Human CD19 (20-291), Fc Tag (Cat. No. CD9-HF251) (QC tested).
Flow Cytometry assay shows that PE-Labeled Human PD-1, Fc Tag, His Tag (recommended for neutralizing assay) (Cat. No. PD1-HP2F2) can bind to 293T cells overexpressing human PD-L1. 1μl stock solution per million cells (QC tested).
FACS analysis shows that the binding of PE-Labeled Human PD-1, Fc Tag, His Tag (recommended for neutralizing assay) (Cat. No.PD1-HP2F2) to 293T overexpressing PD-L1 was inhibited by increasing concentration of neutralizing antihuman PD-L1 antibody. The IC50 is 0.29 μg/ml (QC tested).
Binding activity of three different lots of FITC-labeled Human MSLN, Fc Tag was evaluated in the above FACS analysis against Anti-MSLN CAR-293 cells. The result shows very high batch-to-batch consistency.
Binding activity of three different lots of FITC-labeled Human MSLN, Fc Tag was evaluated in the above ELISA analysis against Anti-MSLN Antibody. The result shows very high batch-to-batch consistency.
Binding activity of the Human MSLN, Fc Tag before and after FITC labeling was evaluated in the above ELISA analysis. The result shows that FITC-Labeled and Unconjugated Human MSLN, Fc Tag have almost the same level of binding activity.
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We are actively developing the Fluorescent-labeled product line to fulfill our customer needs for biologic drug discovery and development. So we are highly appreciated if you would like to share your ideas and needs in this field. Please don’t hesitate to contact us if you have any suggestions or any customized request.
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