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CAR T cell therapy in solid tumors

발행일：2021-09-06

Solid tumors represent approximatively 90% of adult human cancers, and there are many frequently targeted TAAs for solid tumors therapy, including Mesothelin, HER2, EGF R, GPC3, MUC1 and PSMA. The clinical success of CAR T-cell therapy in blood cancers has generated enthusiasm for testing the technology in solid tumors.

Hot targets

MSLN

MSLN is highly expressed in multiple solid tumors, such as ovarian cancer, lung adenocarcinoma, and breast cancer. Considering its low expression on normal mesothelial cells and high expression in cancer cells, MSLN is a promising target of CAR T therapy for solid tumors.

GPC3

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Glypican-3 (GPC3) is an attractive target for CAR-T cell therapy, as it is highly upregulated in hepatocellular carcinoma and several pediatric solid embryonal tumors but shows restricted expression in healthy adult tissues. According to ClinicalTrials.gov, more than 20 clinical trials have been initiated.

MUC16

More than 80% of ovarian cancer is characterized by overexpression of MUC16, and the high expression of MUC16 is an important indicator for the early diagnosis of ovarian cancer. Studies have shown that MUC16-CAR-T cells have specific killing effects on MUC16+ ovarian cancer cells in vitro.

HER2

Overexpression and amplification of HER2 is observed in 5-66% of epithelial ovarian cancer, making it an ideal target for ovarian cancer treatment. At present, HER2-specific CAR-T cell therapy has shown good therapeutic potential in the preclinical stage.

Solid tumor targets at ACROBiosystems

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Data

Detection of Anti-MSLN CAR Expression

Cat. No. MSN-HF223
Product: FITC-Labeled Human Mesothelin (296-580), His Tag

293 cells were transfected with anti-MSLN-scFv and RFP tag. 2e5 of the cells were stained with FITC-Labeled Human Mesothelin (296-580), His Tag (Cat. No. MSN-HF223, 1 μg/mL) and FITC-labeled protein control. Non-transfected 293 cells and FITC-labeled protein control were used as negative control. RFP was used to evaluate CAR (anti-MSLN-scFv) expression and FITC was used to evaluate the binding activity of FITC-Labeled Human Mesothelin (296-580), His Tag (Cat. No. MSN-HF223).

Protocol

FACS Analysis of Anti-HER2 CAR Expression

Cat. No. HE2-HF224
Product: FITC-Labeled Human Her2, His Tag

2e5 of Anti-Her2 CAR-293 were stained with 100 μL of 1 μg/mL of FITC-Labeled Human Her2, His Tag (Cat. No. HE2-HF224) and negative control protein respectively, FITC signal was used to evaluate the binding activity.