칼리크레인(KLK) 패밀리: 종양학에서 떠오르는 표적
| 분자 | 주요 생물학적 기능 |
|---|---|
| KLK1 | Regulates the kallikrein-kinin system; modulates blood pressure and inflammatory responses. |
| KLK4, KLK10, KLK11 | Regulate epithelial cell dynamics (proliferation, differentiation, apoptosis); involved in carcinogenesis. |
| KLK6 | Implicated in CNS inflammation and neurodegenerative disease progression. |
| KLK2, KLK3 | Established prostate cancer biomarkers and therapeutic targets. |
| KLK5, KLK6, KLK7, KLK14 | Core components of the skin barrier protease network; mediate desquamation and barrier homeostasis; linked to inflammatory dermatoses. |
| KLK8, KLK9, KLK12, KLK13, KLK15 | Play emerging roles in inflammation and tumor progression (mechanisms under investigation). |
주요 특성 및 응용 분야
제품 특징
고순도 및 높은 균질성: SDS-PAGE 및 SEC-MALS/HPLC로 검증됨.
높은 효소 활성: 신뢰할 수 있는 데이터 확보를 위해 정기 QC 테스트로 검증됨.
높은 생물학적 활성: ELISA로 검증됨.
로트 간 일관성: 엄격한 기준을 통해 안정성을 확보함으로써 장기적인 신약 개발을 지원.
응용
항체 개발: 면역, 항체 스크리닝 및 기능 연구.
약물 히트-리드 탐색: 리드 화합물 및 억제제 스크리닝.
분석법 개발: 특정 절단 활성을 활용한 기질 기반 검출 분석법 구축.
제품 목록
검증 데이터
Human Kallikrein 2 Protein, Tag Free (Cat.No. KA2-H5214) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
Biotinylated Human Kallikrein 3 Protein, His,Avitag (Cat.No. KA3-H82E4)on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
The purity of Human Kallikrein 3 Protein, His Tag (Cat. No. KL3-H52H3) is more than 95% and the molecular weight of this protein is around 25-40 kDa verified by SEC-MALS.
Protocol
The purity of Human Kallikrein 1 (25-262) Protein, His Tag (Cat. No. KL1-H52H4) was greater than 95% as determined by SEC-HPLC.
Protocol
| Cat. No. |
Human Kallikrein 2 Protein, Tag Free (active enzyme) (Cat.No.KA2-H5214) |
Human Kallikrein 2 Protein, His Tag (active enzyme) (Cat.No.KA2-H52H3) |
| Substrate | Pro-Phe-Arg-7-amido-4-methylcoumarin (PFR-AMC) | |
|
Enzyme Activity （pmol/min/μg） |
>450 | >150 |
| Cat. No. |
Biotinylated Human Kallikrein 3 Protein, His,Avitag™ (active enzyme, MALS verified) (Cat.No.KA3-H82E4) |
Human Kallikrein 3 Protein, His Tag (Pro-form, MALS verified) (Cat.No.KL3-H52H3) |
| Substrate | Succinyl-Arg-Pro-Tyr-p-Nitroanilide (SucRPYpNA) | |
|
Enzyme Activity （pmol/min/μg） |
>160 | >150 |
| Cat. No. |
Human Kallikrein 5 Protein, Tag Free (active enzyme) (Cat.No.KA5-H5213) |
Human Kallikrein 7 Protein, Tag Free (active enzyme) (Cat.No.KA7-H5213) |
| Substrate | BOC-Val-Pro-Arg-AMC | Mca-RPKPVE-Nval-WRK(Dnp)-NH2 |
|
Enzyme Activity （pmol/min/μg） |
>350 | ＞150 |
Immobilized Human Kallikrein 2 Protein, Tag Free (Cat. No. KA2-H5214) at 1 μg/mL (100 μL/well) can bind Human KLK2 Antibody with a linear range of 0.1-8 ng/mL (Routinely tested).
Protocol
Immobilized Human Kallikrein 2 Protein, His Tag (Cat. No. KA2-H52H3) at 1 μg/mL (100 μL/well) can bind Human KLK2 Antibody, IgG with a linear range of 0.06-2 ng/mL (Routinely tested).
Protocol
- 배경
- 주요 특성 및 응용 분야
- 제품 목록
- 검증 데이터
- 관련 제품