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Product Specifications
Assay TypeSandwich-ELISAAnalyteAAV8Format96TRegulatory StatusRUOSensitivity<1.75E+07 capsids/mLStandard Curve Range5.60E+08 capsids/mL-1.75E+07 capsids/mLAssay Time3 hr 20 minSuitable Sample TypeFor the detection and quantitative determination of AAV8 capsid titration in AAV gene therapy product preparation processing.Sample volume100 μLProduct Overview
AAV8 Titration ELISA Kit is based on the sandwich-ELISA method and is used to detect and quantitatively determine the AAV8 capsid titration in AAV gene therapy product winthin 3 hr 20 min. The kit is designed to provide reliable solutions for the early development, production, and quality control of AAV gene therapy products.
Reconstitution
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
Storage
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeAAV08-C01Pre-coated Anti-AAV8 Antibody Microplate1 plateAAV08-C02AAV8 Standard1.4E+10 capsidsAAV08-C03Biotin-Anti-AAV8 Antibody15 μgAAV08-C04Streptavidin-HRP50 μLAAV08-C0510×Washing Buffer50 mLAAV08-C062×Dilution Buffer50 mLAAV08-C07Substrate Solution12 mLAAV08-C08Stop Solution7 mLACRO Quality Management System
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Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
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