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Your Position: > Protein > B7-H7 > B77-H82F3

Biotinylated Human B7-H7 / HHLA2 Protein, Fc,Avitag™ (MALS verified)

  • Synonym
    B7-H7,HHLA2,B7 Homolog 7
  • Source
    Biotinylated Human B7-H7 Protein, Fc,Avitag(B77-H82F3) is expressed from human 293 cells (HEK293).
  • Molecular Characterization
    B7-H7 Structure

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™)

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 51 mM Tris,100 mM Glycine,25 mM Arginine,150 mM NaCl with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
B7-H7 SDS-PAGE

Biotinylated Human B7-H7 Protein, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
B7-H7 MALS images

The purity of Biotinylated Human B7-H7 Protein, Fc,Avitag (Cat. No. B77-H82F3) is more than 90% and the molecular weight of this protein is around 155-175kDa verified by SEC-MALS.

Bioactivity-ELISA
 B7-H7 ELISA

Immobilized Human CD28H, Fc Tag (Cat. No. CDH-H5251) at 5 μg/mL (100 μL/well) can bind Biotinylated Human B7-H7 Protein, Fc,Avitag (Cat. No. B77-H82F3) with a linear range of 1-20 ng/mL (QC tested).

 B7-H7 ELISA

Immobilized Human CD28H, His Tag (Cat. No. CDH-H52H3) at 5 μg/mL (100 μL/well) can bind Biotinylated Human B7-H7 Protein, Fc,Avitag (Cat. No. B77-H82F3) with a linear range of 1-78 ng/mL (Routinely tested).

  • Background
    B7-H7 (HHLA2) is a newly identified B7 family member that regulates human T-cell functions. B7-H7 was previously known as human endogenous retrovirus-H long terminal repeat associating 2 (HHLA2) with unidentified function. Recently, B7-H7 has been identified as a specific ligand for human CD28H. The B7-H7-CD28H pathway strongly promoted CD4+ T-cell proliferation and cytokine production via an AKT-dependent signaling cascade in the presence of TCR signaling, suggesting B7-H7 comprises a new co-stimulatory pathway. The first IgV domain of B7-H7, which presumably binds to a putative receptor, shows the highest homology to other B7 family members.
  • Clinical and Translational Updates

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