로그인 회원가입
icon_bulk_order대량 주문 Acrobiosystems for English
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: > CAR-T > Siglec-3 > CD3-H82F3

Biotinylated Human Siglec-3 / CD33 Protein, Fc,Avitag™ (MALS verified)

인기 제품 추천: SP-11 스트렙타비딘 코팅된 96웰 플레이트  샘플 신청 
STN-N5116 스트렙타비딘 단백질  SMB-B01 스트렙타비딘 마그네틱 비드 
비오틴-스트렙타비딘 시스템의 높은 특이성과 고감도를 이용하여 비오틴화된 단백질을 스트렙타비딘 관련 제품과 결합하여 다양한 실험에서 사용할 수 있습니다. 비비오틴 시스템과 비교하면 검출이 더 빠르고 더 강력하고 더 낮은 비특이적 배경을 가지고 있습니다.
  • 동의어
    CD33,SIGLEC3,gp67
  • 출처
    Biotinylated Human Siglec-3,Fc,Avitag (CD3-H82F3) is expressed from human 293 cells (HEK293). It contains AA Asp 18 - His 259 (Accession # AAH28152.1).
    Predicted N-terminus: Asp 18
  • 분자 표징
    Online(Asp 18 - His 259) AAH28152.1

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 54.9 kDa. The protein migrates as 70-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 비오틴화
    비오틴화 of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • 내독소
    Less than 1.0 EU per μg by the LAL method.
  • 순도

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • 레시피

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • 재구성

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 보관

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human Siglec-3,Fc,Avitag (Cat. No. CD3-H82F3) SDS-PAGE gel

Biotinylated Human Siglec-3,Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated Human Siglec-3,Fc,Avitag (Cat. No. ) MALS images

The purity of Biotinylated Human Siglec-3,Fc,Avitag (Cat. No. CD3-H82F3) is more than 90% and the molecular weight of this protein is around 155-185 kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated Human Siglec-3,Fc,AvitagBiotinylated Human Siglec-3,Fc,Avitag (Cat. No. CD3-H82F3) ELISA bioactivity

Immobilized Biotinylated Human Siglec-3,Fc,Avitag (Cat. No. CD3-H82F3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-Siglec-3 Antibody, Human IgG1 with a linear range of 0.2-2 ng/mL (QC tested).

  • 백그라운드
    Myeloid cell surface antigen CD33 is also known as SIGLEC3, Siglecs (sialic acid binding Ig­like lectins) and GP67, is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. Human CD33 / Siglec-3 cDNA encodes a 364 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-­terminal Ig-­like V­-type domain, one Ig­-like C2-­type domains, a transmembrane region and a cytoplasmic tail. CD33 / Siglec-3 usually considered myeloid-specific, but it can also be found on some lymphoid cells. In the immune response, CD33 / Siglec-3 may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. CD33 / Siglec-3 induces apoptosis in acute myeloid leukemia.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

코멘트 (0)


ETD of in-stock products: 4 business days

가격(KRW) : 525,000

가격(KRW) : 2,280,000

Produit en vogue recommandé
스트렙타비딘 코팅판

가격(KRW) : 45,000

가격(KRW) : 195,000

스트렙타비딘-HRP

가격(KRW) : 36,000

가격(KRW) : 270,000

Magnetic Beads™ 스트렙타비딘

가격(KRW) : 315,000

가격(KRW) : 525,000

보급 및 전시



약물 개발 현황

  • Number of Launched Drugs:1 Details
  • Number of Drugs in Clinical Trials:22 Details
  • Latest Research Phase:Approved

데이터 테이블 및 파일

DMF 신청
DMF (Drug Master File)

연락처

+1  800-810-0816 (US)
+86 400-682-2521 (AP)

상관 제품

신제품

의문 과 코맨트

This web search service is supported by Google Inc.

totop