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Your Position: > Kits > CD73 > CEA-B048

ClinMax™ Human Soluble CD73 / NT5E ELISA Kit, PRO

For research use only.
Product Details
Assay TypeSandwich-ELISA
AnalyteSoluble NT5E/CD73
Format96T
ReactivityHuman
Regulatory StatusPRO
Sensitivity100 pg/mL
Standard Curve Range123 pg/mL-30000 pg/mL
Assay Time3hr
Suitable Sample TypeFor the quantitative determination of human Soluble NT5E/CD73 in Cell Culture Supernatants, Plasma, Serum.
Sample volume100 μL
Materials Provided
IDComponentsSize
CEA048-C01Pre-coated Anti- Soluble NT5E/CD73 Antibody Microplate1 plate
CEA048-C02Human Soluble NT5E/CD73 Standard123μg ×2
CEA048-C03Biotin-Anti-Soluble NT5E/CD73 Antibody Con. Solution200 μL
CEA048-C04Biotin-Antibody Dilution Buffer8 mL
CEA048-C05Streptavidin-HRP Con. Solution500 uL
CEA048-C06Streptavidin-HRP Dilution Buffer15 mL
CEA048-C0720× Washing Buffer50 mL
CEA048-C08Sample Dilution Buffer15 mL ×2
CEA048-C09Substrate Solution12 mL
CEA048-C10Stop Solution6 mL
  • Background
    ClinMax™ ELISA Kit is convenient ready-to-use immunoassay Kit, specifically designed to quantitate human NT5E/CD73 that is present in complex biological samples, such as human serum, plasma, and cell culture supernates.

    A comprehensive validation of the ELISA method was performed following the ICH M10 on bioanalytical method validation and the FDA’s bioanalytical method validation guidance for industry. This validation included assessments of linearity, accuracy, precision, dilution linearity, recovery, and the hook effect. For details information, please refer to the DS.

    ClinMax™ ELISA Kits are manufactured in a GMP-certified facility and comply to the ISO 13485 standard, ensuring a high level of quality and reliability.

  • Application

    The kit is developed for quantitative detection of human soluble NT5E/CD73 in serum, plasma and cell culture supernates.

    It is for research use only.

  • Storage
    Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
  • Assay Principles
     CD73 Assay Principles
Typical Data Please refer to Ds document for the assay protocol.
 CD73 TYPICAL DATA

For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of NT5E/CD73 is less than 100 pg/mL.

Validation
Intra-Assay Statistics

Ten replicates of each of 3 samples containing different NT5E/CD73 concentrations were tested in one assay. Acceptable criteria: CV<10%.

 CD73 INTRA-ASSAY STATISTICS
Inter-Assay Statistics

3 samples containing different concentrations of NT5E/CD73 were tested in independent assays. Acceptable criteria: CV<15%.

 CD73 INTER-ASSAY STATISTICS
Recovery

Recombinant NT5E/CD73 was spiked into 3 human serum samples, and then analyzed. The average recovery of NT5E/CD73 for serum samples is 93.0%.

 CD73 RECOVERY
  • Background: CD73
    NT5E (CD73) is a surface enzyme which is expressed on multiple cells. This enzyme mediates the gradual hydrolysis of the autocrine and paracrine danger signals of ATP and ADP to anti-inflammatory adenosine. Immune suppression mediated by adenosinergic pathways is very important for maintaining immune system homeostasis. Immune suppressive functions of T regulatory cells are also dependent on CD73 expression. Treg's generally suppress the immune response. They affect proliferation and function of T cell.
  • Clinical and Translational Updates

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