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Your Position: > Protein > Galectin-9 > LG9-H82H3

Biotinylated Human Galectin-9 / LGALS9 Protein, His,Avitag™ (SPR verified)

  • Synonym
    Galectin-9,LGALS9,Ecalectin
  • Source
    Biotinylated Human Galectin-9, His,Avitag(LG9-H82H3) is expressed from human 293 cells (HEK293). It contains AA Ala 2 - Thr 323 (Accession # O00182-2).
    Predicted N-terminus: His
  • Molecular Characterization
    Galectin-9 Structure

    This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 39.3 kDa. The protein migrates as 52-58 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 20 mM MOPS, 50 mM Nacl, 1 mM EDTA, 2 mM DTT, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Galectin-9 SDS-PAGE

Biotinylated Human Galectin-9, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-SPR
 Galectin-9 SPR

Human TIM-3 (22-200) Protein, Fc Tag (Cat. No. TM3-H5258) immobilized on CM5 Chip can bind Biotinylated Human Galectin-9, His,Avitag (Cat. No. LG9-H82H3) with an affinity constant of 7.66 μM as determined in a SPR assay (Biacore 8K) (QC tested).

  • Background
    Galectin-9 (LGALS9) is also known as tumor antigen HOM-HD-21 and ecalectin. Galectins are a family of proteins defined by their binding specificity for β-galactoside sugars, such as N-acetyllactosamine (Galβ1-3GlcNAc or Galβ1-4GlcNAc), which can be bound to proteins by either N-linked or O-linked glycosylation. As for Galectin-9, it has high affinity for the Forssman pentasaccharide, and it is also the ligand for HAVCR2/TIM3. Forthermore, Galectin-9 stimulates bactericidal activity in infected macrophages by causing macrophage activation and IL1B secretion which restricts intracellular bacterial growth.
  • Clinical and Translational Updates

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