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Your Position: 홈 > Protein > PD-1 > PD1-C5254

Cynomolgus PD-1 / PDCD1 Protein, Fc Tag (MALS verified)

INTRODUCTION
QC & Assay Protocol
Citation & Background
  • Synonym
    PDCD1,PD1,CD279,SLEB2
  • Source
    Cynomolgus PD-1, Fc Tag (PD1-C5254) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Gln 167 (Accession # B0LAJ3).
    Predicted N-terminus: Leu 25
    Request for sequence
  • Molecular Characterization
    Online(Leu 25 - Gln 167) B0LAJ3

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 42.6 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Cynomolgus PD-1, Fc Tag (Cat. No. PD1-C5254) SDS-PAGE gel

Cynomolgus PD-1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Cynomolgus PD-1, Fc Tag (Cat. No. ) MALS images

The purity of Cynomolgus PD-1, Fc Tag (Cat. No. PD1-C5254) is more than 95% and the molecular weight of this protein is around 96-118 kDa verified by SEC-MALS.

Report
Bioactivity-ELISA
Cynomolgus PD-1, Fc TagCynomolgus PD-1, Fc Tag (Cat. No. PD1-C5254) ELISA bioactivity

Immobilized Human PD-L1, Mouse IgG1 Fc Tag, low endotoxin (HPLC-verified) (Cat. No. PD1-H52A3) at 5 μg/mL (100 μL/well) can bind Cynomolgus PD-1, Fc Tag (Cat. No. PD1-C5254) with a linear range of 3-33 ng/mL (QC tested).

Protocol
Cynomolgus PD-1, Fc TagCynomolgus PD-1, Fc Tag (Cat. No. PD1-C5254) ELISA bioactivity

Immobilized Cynomolgus PD-1, Fc Tag (Cat. No. PD1-C5254) at 2 μg/mL (100 μL/well) can bind Nivolumab with a linear range of 0.1-3 ng/mL (Routinely tested).

Protocol
Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Cynomolgus PD-1, Fc Tag (Cat. No.PD1-C5254) on Protein A Biosensor, can bind Cynomolgus / Rhesus macaque PD-L1, His Tag (Cat. No. PD1-C52H4) with an affinity constant of 1.7 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol
Human_FcRn_Heterodimer_Protein_Bli

Loaded Cynomolgus PD-1, Fc Tag (Cat. No. PD1-C5254) on Protein A Biosensor, can bind Human PD-L2, His Tag (SPR verified) (Cat. No. PD2-H5220) with an affinity constant of 0.4 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol
  • Citations
  • Background
    Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-γ secretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer.
  • Clinical and Translational Updates
      

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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