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Your Position: > Protein > Complement C1s > COS-H52H3

Human Complement C1s Protein, His Tag

  • Synonym
    EDSPD2
  • Source
    Human Complement C1s Protein, His Tag(COS-H52H3) is expressed from human 293 cells (HEK293). It contains AA Glu 16 - Asp 688 (Accession # P09871).
    Predicted N-terminus: Glu 16
  • Molecular Characterization
    Complement C1s Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 76.8 kDa. The protein migrates as 30 kDa and 53-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Complement C1s SDS-PAGE

Human Complement C1s Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

Bioactivity

Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB).The specific activity is >8000 pmol/min/μg (QC tested).

  • Background
    This gene encodes a serine protease, which is a major constituent of the human complement subcomponent C1. C1s associates with two other complement components C1r and C1q in order to yield the first component of the serum complement system. Defects in this gene are the cause of selective C1s deficiency.
  • Clinical and Translational Updates

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