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Your Position: > Protein > Hemagglutinin 1/HA 1 (Influenza Virus) > HA1-V52H5

Influenza A [A/guinea fowl/Hong Kong/WF10/99(H9N2)] Hemagglutinin 1 (HA1) Protein, His Tag (MALS verified)

  • Source
    Influenza A [A/guinea fowl/Hong Kong/WF10/99(H9N2)] HA1 Protein, His Tag(HA1-V52H5) is expressed from human 293 cells (HEK293). It contains AA Asp 19 - Arg 338 (Accession # Q80KD9_9INFA).
    Predicted N-terminus: Asp 19
  • Molecular Characterization
    Hemagglutinin 1/HA 1 (Influenza Virus) Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 40.9 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 0.1 M Sodium citrate, pH5.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Hemagglutinin 1/HA 1 (Influenza Virus) SDS-PAGE

Influenza A [A/guinea fowl/Hong Kong/WF10/99(H9N2)] HA1 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Hemagglutinin 1/HA 1 (Influenza Virus) MALS images

The purity of Influenza A [A/guinea fowl/Hong Kong/WF10/99(H9N2)] HA1 Protein, His Tag (Cat. No. HA1-V52H5) is more than 90% and the molecular weight of this protein is around 170-190 kDa verified by SEC-MALS.

Bioactivity-ELISA
 Hemagglutinin 1/HA 1 (Influenza Virus) ELISA

Immobilized Influenza A [A/guinea fowl/Hong Kong/WF10/99(H9N2)] Hemagglutinin 1 (HA1) Protein, His Tag (Cat. No. HA1-V52H5) at 1 μg/mL (100 μL/well) can bind Anti-HA Monoclonal Antibody (H9N2) with a linear range of 0.1-4 ng/mL (QC tested).

  • Background
    Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Hemagglutinin also plays a major role in the determination of host range restriction and virulence. As a class I viral fusion protein, hemagglutinin is responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane.
  • Clinical and Translational Updates

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재고상품 출발날짜: 영업일 기준 4일

가격(KRW) : 525,000

가격(KRW) : 4,020,000

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