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Your Position: > Protein > CD27 Ligand > CDL-HF249

FITC-Labeled Human CD27 Ligand / CD70 Protein, His TagStar Staining

  • Synonym
    CD70,CD27LG,TNFSF7,TNFSF7G,CD27L
  • Source
    FITC-Labeled Human CD27 Ligand Protein, His Tag (CDL-HF249) is produced via conjugation of FITC to Human CD27 Ligand Protein, His Tag with a new generation site-specific technology under Star Staining labeling platform. Human CD27 Ligand Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Ser 52 - Pro 193 (Accession # P32970-1).
    Predicted N-terminus: His
  • Molecular Characterization

    This protein carries a polyhistidine tag at the N-terminus

    The protein has a calculated MW of 63.6 kDa.

  • Conjugate

    FITC

    Excitation source: 488 nm spectral line, argon-ion laser

    Excitation Wavelength: 488 nm

    Emission Wavelength: 535 nm

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
Evaluation of CAR expression
FACS Analysis of Anti-CD27 Ligand CAR Expression
 CD27 Ligand CAR_T

5e5 of anti-CD27 Ligand CAR-293 cells were stained with 100 μL of 3 μg/mL of FITC-Labeled Human CD27 Ligand Protein, His Tag (Cat. No. CDL-HF249) and negative control protein respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A), FITC signal was used to evaluate the binding activity (QC tested).

  • Background
    Cluster of Differentiation 70 (CD70) is also known as CD27 ligand (CD27L / CD27LG), TNFSF7, TNFSF7G, is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and is a surface antigen found on activated T-and B-lymphocytes and mature dendritic cells. Binding of CD70 to its receptor CD27 induces in priming, effector functions, differentiation and memory formation of T-cells, and thus is invloved in the biological processes including T-cell activation, the proliferation of costimulated T-cells, as well as the generation of cytolytic T-cells. CD70 on T cells provides costimulatory signals that are required for T cell proliferation, clonal expansion and the promotion of effector T cell formation. CD70 on mouse B cell has been shown to inhibit terminal differentiation of activated B cells into plasma cells and enhances commitment to memory B cell responses. CD70 induces proliferation and IFNγ production, on NK cells.
  • Clinical and Translational Updates

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