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Your Position: > Protein > CXCL9 > CX9-H51H3

Human CXCL9 Protein, His Tag

  • Synonym
    CMK, Humig, MIG, SCYB9, crg-10
  • Source
    Human CXCL9 Protein, His Tag(CX9-H51H3) is expressed from E. coli cells. It contains AA Thr 23 - Thr 125 (Accession # Q07325).
    Predicted N-terminus: Met
  • Molecular Characterization
    CXCL9 Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 20.7 kDa. The protein migrates as 17-20 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, 0.5 M NaCl, 0.5 M Arginine, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
CXCL9 SDS-PAGE

Human CXCL9 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

Bioactivity-ELISA
 CXCL9 ELISA

Immobilized Human CXCL9 Protein, His Tag (Cat. No. CX9-H51H3) at 5 μg/mL (100 μL/well) can bind Human CXCR3 Protein, Flag,His Tag (Cat. No. CX3-H52D5) with a linear range of 0.019-0.313 μg/mL (QC tested).

  • Background
    CXCL9 is a small cytokine belonging to the CXC chemokine family that is also known as monokine induced by gamma interferon (MIG). CXCL9 is a chemokine that plays a role in immune responses, it can induce chemotaxis, promote differentiation and proliferation of leukocytes, and cause tissue extravasation. CXCL9 regulates immune cell migration, differentiation and activation by binding to CXCR3 receptor, such as CTLs, NK cells, NKT cells and macrophages. CXCL9 also participates in Th1 polarization and IFN-γ response.
  • Clinical and Translational Updates

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DMF (Drug Master File)

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