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Your Position: > Protein > Mesothelin > MSN-H5267

Human Mesothelin / MSLN (391-598) Protein, Fc Tag (MALS verified)

  • Synonym
    MSLN,Mesothelin,MPF
  • Source
    Human Mesothelin (391-598), Fc Tag(MSN-H5267) is expressed from human 293 cells (HEK293). It contains AA Ser 391 - Ser 598 (Accession # AAH09272.1).
    Predicted N-terminus: Pro
  • Molecular Characterization
    Mesothelin Structure

    This protein carries a human IgG1 Fc tag at the N-terminus.

    The protein has a calculated MW of 49.6 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.2 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Mesothelin SDS-PAGE

Human Mesothelin (391-598), Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Mesothelin MALS images

The purity of Human Mesothelin (391-598), Fc Tag (Cat. No. MSN-H5267) is more than 90% and the molecular weight of this protein is around 95-120 kDa verified by SEC-MALS.

Bioactivity-ELISA
 Mesothelin ELISA

Immobilized Human Mesothelin (391-598), Fc Tag (Cat. No. MSN-H5267) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human MSLN Antibody, Human IgG1 with a linear range of 0.156-5 μg/mL (QC tested).

  • Background
    Mesothelin (MSLN) is also known as CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, which belongs to the mesothelin family. Mesothelin / MSLN can be proteolytically cleaved into the following two chains by a furin-like convertase: Megakaryocyte-potentiating factor (MPF) and the cleaved form of mesothelin. Both MPF and the cleaved form of mesothelin are N-glycosylated. Mesothelin / MSLN can interacts with MUC16. The membrane-anchored forms of MSLN may play a role in cellular adhesion. MPF potentiates megakaryocyte colony formation in vitro.
  • Clinical and Translational Updates

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