로그인 회원가입
icon_bulk_order대량 주문 Acrobiosystems for English
Promotion
Recombinant Proteins ClearClear history
Recombinant Proteins CloseClose
Browse Products By Molecule Name a-zA-Z
TL1A Hot |GMP IL-2 Hot |Claudin 18.2 Hot |FMC 63 Ab  |FcRn
0
장바구니가 비어 있습니다
내 장바구니
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: 홈 > Cell > PD-L1 > SCRAJ-STT075

Raji/Human PD-L1 Stable Cell Line Development Service

INTRODUCTION
QC & Assay Protocol
Citation & Background
  • Description
    The Raji/Human PD-L1 Stable Cell Line was engineered to express full length human PD-L1 (Gene ID: 29126, used to mimic cancer target cells. When co-cultured with human PD-1 Reporter Cell, the PD-1/PD-L1 interaction inhibits TCR signaling and NFAT-mediated luminescence. Blocking the PD-1/PD-L1 interaction by either anti-PD-1 or anti-PD-L1 antibodies releases the inhibitory signal and results in TCR activation and NFAT-mediated luminescence.
  • Application

    Useful for cell-based PD-L1 binding assay

    Useful as PD-L1-expressing target cells in reporter gene assay

    PD-L1 Assay Principles

  • Growth Properties
    Suspension
  • Selection Marker
    Puromycin (2 μg/mL)
  • Culture Medium
    RPMI-1640 + 10% FBS
  • Freeze Medium
    Serum-free cell cryopreservation medium
  • Quantity
    1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
  • Storage
    Frozen in liquid nitrogen.
  • Mycoplasma Testing
    Negative
  • Sterility Testing
    Negative
  • Instructions for Use
    See data sheet for detailed culturing and assay protocol.
Receptor Assay
PD-L1 FACS

Expression analysis of human PD-L1 on Raji/Human PD-L1 Stable Cell by FACS.
Raji/Human PD-L1 Stable Cell Line or negative control cell were stained with PE-labeled anti-human PD-L1 antibody.

Protocol
Application
PD-L1 APPLICATION

Blocking activity of anti-human PD-1 antibody (RLU).
This Raji/Human PD-L1 Stable Cell Line was incubated with serial dilutions of antibodies in the presence of reporter cells expressing human PD-1. The EC50 of anti-human PD-1 antibody was approximately 1.189 μg/mL.

Protocol
PD-L1 APPLICATION

Blocking activity of anti-human PD-1 antibody (FOLD).
This Raji/Human PD-L1 Stable Cell Line was incubated with serial dilutions of antibodies in the presence of reporter cells expressing human PD-1. The max induction fold was approximately 22.47.

Protocol

Please contact us if you are interested in related cell pool service.

  • Background
    Programmed cell death 1 ligand 1 (PDL1) is also known as B7-H, B7H1, MGC142294, MGC142296, PD-L1, PDCD1L1 and PDCD1LG1,which is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses.PDL1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. This protein is broadly expressed in the majority of peripheral tissues as well as hematopoietic cells. Interaction between PDL1 and its receptors belonging to the CD28 family of molecules provide both stimulatory and inhibitory signals in regulating T cell activation and tolerance. PDL1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression.
  • License Disclosure
    This cell line is provided for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit modification of this cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license. Modifications of this cell line, transfer to another facility, or commercial use of the cell lines may require a separate license and additional fees. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.
  • Clinical and Translational Updates

코멘트 (0)


재고상품 출발날짜: 영업일 기준 4일

가격(KRW) : 5,250,000

장바구니에 담기 대량구매 맞춤형 제품

Antibody Licensing

more

보급 및 전시



약물 개발 현황

  • Number of Launched Drugs：7 Details
  • Number of Drugs in Clinical Trials：147 Details
  • Latest Research Phase：NDA/BLA
다른 추천 제품

Datasheet & Documentation

연락처

+1  800-810-0816 (US)
+86 400-682-2521 (AP)
Show All Distributors

관련 제품

신제품

문의와 컨텐츠

This web search service is supported by Google Inc.

totop
Call us
우리에게 전화하세요
글로벌: +1 800-810-0816 (무료)
아시아태평양: +86 400-682-2521
Email
이메일
order.kr@acrobiosystems.com
Fax
팩스
+1 888-377-6111
Address
주소
서울 성동구 왕십리로10길 6 서울숲비즈포레 703
회사명
아크로바이오 주식회사
사업자 등록번호
186-88-02858
대표자
CHEN YIDING
사업장주소
서울특별시 송파구 중대로12길 10-20, 1층 셀렉티드송파(가락동)
linkedin twitter facebook youtube

문의

Copyright © 2017 ACROBiosystems. All Rights Reserved
Related Links|Privacy Policy|Terms and Conditions|Legal|Sitemap