로그인 회원가입
icon_bulk_order대량 주문 Acrobiosystems for English
0
장바구니가 비어 있습니다
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: > Protein > B7-1 > B71-H5259

Human B7-1 / CD80 Protein, Fc Tag (MALS verified)

  • Synonym
    CD80,B7,B7-1,B7.1,BB1,CD28LG,CD28LG1,LAB7
  • Source
    Human B7-1, Fc Tag (B71-H5259) is expressed from human 293 cells (HEK293). It contains AA Val 35 - Asn 242 (Accession # NP_005182.1).
    Predicted N-terminus: Val 35
  • Molecular Characterization
    Online(Val 35 - Asn 242) NP_005182.1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 50.0 kDa. The protein migrates as 65-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human B7-1, Fc Tag (Cat. No. B71-H5259) SDS-PAGE gel

Human B7-1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human B7-1, Fc Tag (Cat. No. ) MALS images

The purity of Human B7-1, Fc Tag (Cat. No. B71-H5259) is more than 95% and the molecular weight of this protein is around 125-135 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human B7-1, Fc TagHuman B7-1, Fc Tag (Cat. No. B71-H5259) ELISA bioactivity

Immobilized Human B7-1, Fc Tag (Cat. No. B71-H5259) at 2 μg/mL (100 μL/well) can bind Human CTLA-4, His Tag (Cat. No. CT4-H52H9) with a linear range of 0.1-2 ng/mL (QC tested).

Human B7-1, Fc TagHuman B7-1, Fc Tag (Cat. No. B71-H5259) ELISA bioactivity

Immobilized Human B7-1, Fc Tag (Cat. No. B71-H5259) at 5 μg/mL (100 μL/well) can bind Biotinylated Human PD-L1, Fc,Avitag,His Tag (Cat. No. PD1-H82F3) with a linear range of 0.02-1.25 μg/mL (Routinely tested).

Human B7-1, Fc TagHuman B7-1, Fc Tag (Cat. No. B71-H5259) ELISA bioactivity

Immobilized Human B7-1, Fc Tag (Cat. No. B71-H5259) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CTLA-4, Fc,Avitag (Cat. No. CT4-H82F3) with a linear range of 0.6-10 ng/mL (Routinely tested).

Human B7-1, Fc TagHuman B7-1, Fc Tag (Cat. No. B71-H5259) ELISA bioactivity

Immobilized Biotinylated Mouse CD28, His,Avitag (Cat. No. CD8-M82E3) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Human B7-1, Fc Tag (Cat. No. B71-H5259) with a linear range of 0.2-2 ng/mL (Routinely tested).

Human B7-1, Fc TagHuman B7-1, Fc Tag (Cat. No. B71-H5259) ELISA bioactivity

Immobilized Biotinylated Human / Cynomolgus / Rhesus macaque CD28, His,Avitag (Cat. No. CD8-H82E5) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Human B7-1, Fc Tag (Cat. No. B71-H5259) with a linear range of 0.2-5 ng/mL (Routinely tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Immobilized Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (Cat. No. CD8-H525a) on CM5 Chip can bind Human B7-1, Fc Tag (Cat. No. B71-H5259) with an affinity constant of 2.01 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Human B7-1, Fc Tag (Cat. No. B71-H5259) on Protein A Biosensor, can bind Human / Cynomolgus / Rhesus macaque CD28, His Tag (Cat. No. CD8-H52Hc) with an affinity constant of 5.1 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Human_FcRn_Heterodimer_Protein_Bli

Loaded Human B7-1, Fc Tag (Cat. No. B71-H5259) on Protein A Biosensor, can bind Human CTLA-4, His Tag, active dimer (Cat. No. CT4-H52H9) with an affinity constant of 0.498 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Bioactivity-FACS
FACS assay shows that recombinant Biotinylated FACS

FACS assay shows that Human B7-1, Fc Tag (Cat. No. B71-H5259) can bind to 293T cells overexpressing human CTLA4 protein. The concentration of Human B7-1 is 1 μg/mL (Routinely tested).

코멘트 (0)


재고상품 출발날짜: 영업일 기준 4일

가격(KRW) : 390,000

가격(KRW) : 2,910,000

보급 및 전시



약물 개발 현황

  • Number of Launched Drugs:2 Details
  • Number of Drugs in Clinical Trials:6 Details
  • Latest Research Phase:Approved

Datasheet & Documentation

DMF 신청
DMF (Drug Master File)

신제품

문의와 컨텐츠

This web search service is supported by Google Inc.

totop