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Your Position: 홈 > Kits > TNF-alpha > CRS-D002

Human Tumor Necrosis Factor Alpha (TNF-α) ELISA Assay Pair

For research use only.
    Materials Provided
    IDComponentsSize
    CRD002-C01Human TNF-α Capture Antibody60 μg/5plates
    110 μg/20plates
    CRD002-C02Human TNF-α Standard30 μg/5plates
    60 μg/20plates
    CRD002-C03Human TNF-α Detection Antibody30 μg/5plates
    210 μg/20plates
    CRD002-C04Streptavidin-HRP100 μL/5plates
    120 μL/20plates
  • Background
    Tumor necrosis factor alpha (TNFα) is a cytokine produced primarily by monocytes and macrophages. It is found in synovial cells and macrophages in the tissues.The primary role of TNFα is in the regulation of immune cells. TNFα is able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNFα production has been implicated in a variety of human diseases, including major depression, Alzheimer's disease and cancer. This ELISA assay pair contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human TNF-α.
  • Application

    The pair is developed for quantitative detection of TNF-α in human serum and cell culture supernates.

    It is for research use only.

  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage
    Unopened kit should be stored at 2°C -8°C upon receiving.

    Find the expiration date on the outside packaging and do not use reagents past their expiration date.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
    This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of Human TNF-α. The kit consists of Human TNF-α Capture Antibody and Human TNF-α Standard and Human TNF-α Detection Antibody and Streptavidin-HRP.

    Your experiment will include 5 simple steps:

    a) Coat the plate with Human TNF-α Capture Antibody.

    b) Add your molecule of interest to the tests.

    c) Add Human TNF-α Detection Antibody.

    d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.

    e) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis.

Typical Data Please refer to DS document for the assay protocol.
TNF-alpha TYPICAL DATA

For each experiment, each ELISA plate needs to set the standard curve. The following CRS-D002 standard curve data is for reference only.

  • Clinical and Translational Updates

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