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Your Position: 홈 > Antibody > TNF-alpha > TNA-AM494

Monoclonal Anti-TNF-alpha Antibody, Human IgG1 (16H5) (MALS verified)

INTRODUCTION
QC & Assay Protocol
Citation & Background
  • Source

    Monoclonal Anti-TNF-alpha Antibody, Human IgG1 (16H5) is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain.

  • Clone

    16H5

  • Isotype

    Human IgG1 | Kappa

  • Conjugate

    Unconjugated

  • Antibody Type

    Recombinant Monoclonal

  • Reactivity

    Human

  • Immunogen

    Recombinant Human TNF-alpha derived from human HEK293 cells

  • Specificity

    This product is a specific antibody specifically reacts with TNF-alpha.

  • Application
    ApplicationRecommended Usage
    ELISA0.1-10 ng/mL
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Purification

    Protein A purified/ Protein G purified

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
TNF-alpha SDS-PAGE

Monoclonal Anti-TNF-alpha Antibody, Human IgG1 (16H5) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
TNF-alpha MALS images

The purity of Monoclonal Anti-TNF-alpha Antibody, Human IgG1 (16H5) (Cat. No. TNA-AM494) is more than 90% and the molecular weight of this protein is around 135-160 kDa verified by SEC-MALS.

Report
Bioactivity-ELISA
TNF-alpha ELISA

Immobilized Human TNF-alpha Protein, His Tag (active trimer) (MALS verified) (Cat. No. TNA-H5228) at 2μg/mL (100μL/well) can bind Monoclonal Anti-TNF-alpha antibody, Human IgG1 (16H5) (Cat. No. TNA-AM494) with a linear range of 0.04-1.25 ng/mL (QC tested).

Protocol
Bioactivity-SPR
TNF-alpha SPR

Monoclonal Anti-TNF-alpha antibody, Human IgG1 (16H5) (Cat. No. TNA-AM494) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human TNF-alpha, His Tag (Cat. No. TNA-H5228) with an affinity constant of 0.132 nM as determined in a SPR assay (Biacore 8K) (Routly tested).

Protocol
Bioactivity-BLI
TNF-alpha BLI

Loaded Monoclonal Anti-TNF-alpha antibody, Human IgG1 (16H5) (Cat. No. TNA-AM494) on Protein A Biosensor, can bind Human TNF-alpha, His Tag (active trimer) (MALS verified) (Cat. No. TNA-H5228) with an affinity constant of 1.72 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol
TNF-alpha BLI

Loaded Monoclonal Anti-TNF-alpha antibody, Human IgG1 (16H5) (Cat. No. TNA-AM494) on Protein A Biosensor, can bind Human TNF-alpha, premium grade (MALS verified) (Cat. No. TNA-H4211) with an affinity constant of 2.61 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol
  • Background
    Tumor necrosis factor alpha (TNFα) is a cytokine produced primarily by monocytes and macrophages. It is found in synovial cells and macrophages in the tissues.The primary role of TNFα is in the regulation of immune cells. TNFα is able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNFα production has been implicated in a variety of human diseases, including major depression, Alzheimer's disease and cancer. Recombinant TNFα is used as an immunostimulant under the INN tasonermin. TNFα can be produced ectopically in the setting of malignancy and parallels parathyroid hormone both in causing secondary hypercalcemia and in the cancers with which excessive production is associated.
  • Clinical and Translational Updates

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