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Your Position: > Protein > Spike protein > SPN-S82E3

Biotinylated SARS Spike protein (R667A, K968P, V969P), His,Avitag™ (MALS verified)

  • Synonym
    Spike,S protein,Spike glycoprotein,S glycoprotein
  • Source
    Biotinylated SARS Spike protein, His,Avitag(SPN-S82E3) is expressed from human 293 cells (HEK293). It contains AA Ser 14 - Pro 1195 (Accession # AAP13567.1 (R667A, K968P, V969P)).
    Predicted N-terminus: Ser 14
  • Molecular Characterization
    Spike protein Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 138.0 kDa. The protein migrates as 160-200 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Spike protein SDS-PAGE

Biotinylated SARS Spike protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Spike protein MALS images

The purity of Biotinylated SARS Spike protein, His,Avitag (Cat. No. SPN-S82E3) is more than 90% and the molecular weight of this protein is around 518-572 kDa verified by SEC-MALS.

Bioactivity-ELISA
 Spike protein ELISA

Immobilized Biotinylated SARS Spike protein, His,Avitag (Cat. No. SPN-S82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.005-0.313 μg/mL (QC tested).

 Spike protein ELISA

Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 5 μg/mL (100 μL/well) can bind Biotinylated SARS Spike protein, His,Avitag (Cat. No. SPN-S82E3) with a linear range of 0.01-0.625 μg/mL (Routinely tested).

  • Background
    The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
  • Clinical and Translational Updates

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약물 개발 현황

  • Number of Launched Drugs:1 Details
  • Number of Drugs in Clinical Trials:1 Details
  • Latest Research Phase:Approved

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DMF (Drug Master File)

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