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Your Position: > Protein > EndoS > ENS-S5143

Endo S (200U/μl)

  • Product Details
    This product is an IgG-specific endoglycosidase hydrolyzing complex N-glycans at the Fc N-glycosylation sites. It is derived from Streptococcus pyogenes and expressed in E. coli. The enzyme contains a His-tag and the molecular weight is 110 kDa.The enzyme deglycosylates IgG after the core GlcNAc and display limited activity on high-mannose and hybrid-type glycans.
  • Application
    1. Endo S is specific for N-glycans attached on the Fc-domain of IgGs, and hydrolyzes Fc-glycans of all human IgG subclasses and IgG from many other species, including mouse, rat, monkey, sheep, goat, cow, and horse.
  • Unit Definition
    One unit deglycosylates ≥ 95% of 1 μg human IgG, when incubated in 10 mM sodium phosphate, 150 mM NaCl, pH7.4 at 37°C for 30 min.
  • Quility Control
    Less than 1.0 EU per μg by the LAL method.
  • Formulation

    Supplied as 0.2 μm filtered solution in PBS, pH7.4 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    This product is stable after storage at:

    1. The product MUST be stored at -20°C or lower upon receipt.
    2. -20°C for 6 months under sterile conditions.
Bioactivity
 EndoS ENZYME

100 unit EndoS deglycosylates ≥ 95% of 100 µg IgG when incubated in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4 at 37°C for 30 min.

  • Clinical and Translational Updates

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Datasheet & Documentation

DMF 신청
DMF (Drug Master File)

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