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Biotinylated Mouse EMMPRIN / CD147 Protein, His,Avitag™ (MALS verified)

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비오틴-스트렙타비딘 시스템의 높은 특이성과 고감도를 이용하여 비오틴화된 단백질을 스트렙타비딘 관련 제품과 결합하여 다양한 실험에서 사용할 수 있습니다. 비비오틴 시스템과 비교하면 검출이 더 빠르고 더 강력하고 더 낮은 비특이적 배경을 가지고 있습니다.
  • 동의어
    Basigin,BSG,5F7,CD147,EMMPRIN,M6,OK,TCSF
  • 출처
    Biotinylated Human EMMPRIN, His,Avitag (CD7-M82E8) is expressed from human 293 cells (HEK293). It contains AA Ala 22 - Ala 211 (Accession # NP_001070652.1).
    Predicted N-terminus: Ala 22
  • 분자 표징
    Online(Ala 22 - Ala 211) NP_001070652.1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 24.3 kDa. The protein migrates as 33-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 비오틴화
    비오틴화 of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • 내독소
    Less than 1.0 EU per μg by the LAL method.
  • 순도

    >90% as determined by SDS-PAGE.

  • 레시피

    Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • 재구성

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 보관

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human EMMPRIN, His,Avitag (Cat. No. CD7-M82E8) SDS-PAGE gel

Biotinylated Human EMMPRIN, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Biotinylated Human EMMPRIN, His,Avitag (Cat. No. ) MALS images

The purity of Biotinylated Human EMMPRIN, His,Avitag (Cat. No. CD7-M82E8) is more than 85% and the molecular weight of this protein is around 28-42kDa verified by SEC-MALS.

  • 백그라운드
    CD147, also known as Basigin (BSG), or extracellular matrix metalloproteinase inducer (EMMPRIN). The human basigin protein contains 269 amino acids that form two heavily glycosylated C2 type immunoglobulin-like domains at the N-terminal extracellular portion. A second form of basigin has also been characterized that contains one additional immunoglobulin-like domain in its extracellular portion. As members of the immunoglobulin superfamily play fundamental roles in intercellular recognition involved in various immunologic phenomena, differentiation, and development, basigin is thought also to play a role in intercellular recognition and regulate several distinct functions, such as spermatogenesis, expression of the monocarboxylate transporter and the responsiveness of lymphocytes. Basigin is a type I integral membrane receptor that has many ligands, including the cyclophilin (CyP) proteins Cyp-A and CyP-B and certain integrins.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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