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Your Position: > Protein > ICAM-1 > IC1-H82E8

Biotinylated Human ICAM-1 / CD54 Protein, His,Avitag™ (MALS verified)

  • Synonym
    ICAM1,BB2,CD54,P3.58
  • Source
    Biotinylated Human ICAM-1, His,Avitag(IC1-H82E8) is expressed from human 293 cells (HEK293). It contains AA Gln 28 - Glu 480 (Accession # P05362-1).
    Predicted N-terminus: Gln 28
  • Molecular Characterization
    ICAM-1 Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)

    The protein has a calculated MW of 53.1 kDa. The protein migrates as 66-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
ICAM-1 SDS-PAGE

Biotinylated Human ICAM-1, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
ICAM-1 MALS images

The purity of Biotinylated Human ICAM-1, His,Avitag (Cat. No. IC1-H82E8) is more than 85% and the molecular weight of this protein is around 65-80kDa verified by SEC-MALS.

Bioactivity-ELISA
 ICAM-1 ELISA

Immobilized Human ITGAL&ITGB2 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT2-H53W3) at 5 μg/mL (100 μL/well) can bind Biotinylated Human ICAM-1, His,Avitag (Cat. No. IC1-H82E8) with a linear range of 0.001-0.625 μg/mL (QC tested).

  • Background
    Inter-Cellular Adhesion Molecule 1 (ICAM-1) is also known as Cluster of Differentiation 54 (CD54), is a member of the immunoglobulin superfamily, and is a cell surface glycoprotein which is typically expressed in low concentrations on endothelial cells and cells of the immune system. The protein encoded by this gene is a type of intercellular adhesion molecule continuously present in low concentrations in the membranes of leukocytes and endothelial cells. Upon cytokine stimulation, the concentrations greatly increase. ICAM-1 can be induced by interleukin-1 (IL-1) and tumor necrosis factor alpha (TNFα) and is expressed by the vascular endothelium, macrophages, and lymphocytes. ICAM-1 is a ligand for LFA-1 (integrin), a receptor found on leukocytes. When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 and then transmigrate into tissues. ICAM-1 has been implicated in subarachnoid hemorrhage (SAH). Levels of ICAM-1 are shown to be significantly elevated in patients with SAH over control subjects in many studies. ICAM-1 expressed by respiratory epithelial cells is also the binding site for rhinovirus, the causative agent of most common colds.
  • Clinical and Translational Updates

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