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Your Position: > CKP > PD-L1 > PD1-H5229

Human PD-L1 / B7-H1 Protein, His Tag (MALS verified)

  • 동의어
    PDL1,PD-L1,CD274,B7-H1,B7H1,B7-H,B7H,PDCD1L1,PDCD1LG1,ADC target
  • 출처
    Human PD-L1, His Tag (PD1-H5229) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 238 (Accession # NP_054862.1).
    Predicted N-terminus: Phe 19
  • 분자 표징
    Online(Phe 19 - Arg 238) NP_054862.1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 26.0 kDa. The protein migrates as 30-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 내독소
    Less than 1.0 EU per μg by the LAL method.
  • 순도

    >98% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • 레시피

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • 재구성

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 보관

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 12 months under sterile conditions after reconstitution.
SDS-PAGE
Human PD-L1, His Tag (Cat. No. PD1-H5229) SDS-PAGE gel

Human PD-L1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%.

SEC-MALS
Human PD-L1, His Tag (Cat. No. ) MALS images

The purity of Human PD-L1, His Tag (Cat. No. PD1-H5229) is more than 90% and the molecular weight of this protein is around 30-50 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human PD-L1, His TagHuman PD-L1, His Tag (Cat. No. PD1-H5229) ELISA bioactivity

Immobilized Human PD-L1, His Tag (Cat. No. PD1-H5229) at 1 μg/mL (100 μL/well) can bind Anti-Human PD-L1 MAb (Human IgG1) with a linear range of 0.1-3 ng/mL (QC tested).

Human PD-L1, His TagHuman PD-L1, His Tag (Cat. No. PD1-H5229) ELISA bioactivity

Immobilized Human PD-L1, His Tag (Cat. No. PD1-H5229) at series of concentration on MonoRabᵀᴹ Anti-His Tag (C-term) Antibody precoated (0.1 μg/well) plate, can bind Human PD-1, Mouse IgG2a Fc Tag (HPLC-verified) (Cat. No. PD1-H5255) at 5 μg/mL (100 μL/well) with a linear range of 10-78 ng/mL (Routinely tested).

Bioactivity-alpha
alpha

Biotinylated Human PD-1, Fc,Avitag (Cat. No. PD1-H82F1) at 1 μg/mL (5 μL/well) can bind Human PD-L1, His Tag (Cat. No. PD1-H5229) with a linear range of 0.02-0.625 μg/mL as determined in a alphaLISA homogeneous assay (Routinely tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Human PD-1, Fc Tag (Cat. No. PD1-H5257) captured on CM5 chip via anti-human IgG Fc antibody, can bind Human PD-L1, His Tag (Cat. No. PD1-H5229) with an affinity constant of 3.6 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

Human_FcRn_Heterodimer_Protein_SPR

Anti-Human PD-L1 Mab (Human IgG1) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human PD-L1, His Tag (Cat. No. PD1-H5229) with an affinity constant of 0.286 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Human PD-1, Fc Tag, low endotoxin (Cat. No. PD1-H5257) on ProteinA Biosensor, can bind Human PD-L1, His Tag (Cat. No. PD1-H5229) with an affinity constant of 5.3 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).

Human_FcRn_Heterodimer_Protein_Bli

Loaded Anti-Human PD-L1 MAb (Human IgG1) on AHC Biosensor, can bind Human PD-L1, His Tag (Cat. No. PD1-H5229) with an affinity constant of 1.61 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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ETD of in-stock products: 4 business days

가격(KRW) : 495,000

가격(KRW) : 3,630,000

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