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Your Position: > TME Relevant Pathway > Laminin 511 E8 > LA8-H5283

Human Laminin 511 E8 Protein, Fc Tag

  • Synonym
    Laminin 511 E8, LN-511-E8, Laminin-511 E8
  • Source
    Human Laminin 511 E8, Fc Tag(LA8-H5283) is expressed from human 293 cells (HEK293). It contains AA Glu 2655 - Ala 3327 (Laminin alpha5E8) & Asp 1714 - Leu 1786 (Laminin beta1E8) & Asp 1528 - Pro 1609 (Laminin gamma1E8) (Accession # O15230-1 (Laminin alpha5E8) & P07942-1 (Laminin beta1E8) & P11047-1 (Laminin gamma1E8)).
    Predicted N-terminus: Glu 2655 (Laminin alpha5E8) & Pro (Laminin beta1E8) & Pro (Laminin gamma1E8)
  • Molecular Characterization

    Human Laminin 511 E8, Fc Tag, produced by co-expression of Laminin alpha5E8, Laminin beta1E8 and Laminin gamma1E8, has a calculated MW of 76.7 kDa, 35.0 kDa and 35.7 kDa. The non-reducing (NR) protein migrates as 40-46 kDa (subunit Laminin beta1E8 and subunit Laminin gamma1E8) and 80 kDa (subunit Laminin alpha5E8) respectively due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Laminin 511 E8 SDS-PAGE

Human Laminin 511 E8, Fc Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
 Laminin 511 E8 ELISA

Immobilized Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W7) at 5 μg/mL (100 μL/well) can bind Human Laminin 511 E8, Fc Tag (Cat. No. LA8-H5283) with a linear range of 0.1-4 ng/mL (QC tested).

 Laminin 511 E8 ELISA

Immobilized Human ITGA3&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52Wc) at 5 μg/mL (100 μL/well) can bind Human Laminin 511 E8, Fc Tag (Cat. No. LA8-H5283) with a linear range of 0.1-4 ng/mL (Routinely tested).

  • Background
    Laminin 511 E8 is a truncated laminin 511, which is one of the ECM proteins. laminin E8s serve as functionally minimal forms that retain the full capability for binding to integrins. Laminin 511-E8 consists of the C-terminal end of the alpha 5, beta 1, and gamma 1 chains. The laminin-511 heterotrimer (α5β1γ1) is one of the isoforms to be identified and a potent adhesive and pro-migratory substrate for a variety of normal and tumor cell lines in vitro. Previous studies indicated that laminin-511 contributes to tumor dissemination and metastasis in advanced breast carcinomas and other tumor types. Additionally, latest research uncovered that Laminin 511 might be a target antigen and involved in autoimmune pancreatitis.
  • Clinical and Translational Updates

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