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Your Position: 홈 > Beads > HGF R > MBS-K021

Human HGF R-coupled Magnetic Beads

ItemsSize （2mg）Size（5mg X 2）
Particle size2 μm2 μm
Physical appearancePowder mixturePowder mixture
Amount of Coupled Protein≈192 pmol (20 μg) HGF R protein/mg beads≈192 pmol (20 μg) HGF R protein/mg beads
Binding Capacity>245 pmol (20 μg) HGF/mg beads>245 pmol (20 μg) HGF/mg beads
FormulationPBS, pH7.4, with 10% TrehalosePBS, pH7.4, with 10% Trehalose
Reconstitution2 mL sterile deionized water (1 mg beads/mL)5 mL sterile deionized water (1 mg beads/mL)
  • Background
    The Human HGF R-coupled Magnetic Beads is produced by coupling biotinylated HGF R protein to streptavidin-conjugated magnetic beads. Because Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10-14 mol/L, the biotinylated protein can bind to the SA beads irreversibly. The pre-coupled beads are ready to use for capturing anti-HGF R antibody or HGF from your sample with high specificity.
  • Source
    Human HGF R Protein is expressed from human 293 cells (HEK293). It contains AA Glu 25 - Thr 932(Accession # P08581).
  • Application
    This product is intended for immunocapture, biopanning and flow cytometry. This product is produced non-sterile.
    *The Isotype control (Cat. No. SMB-B01) is sold separately.
  • Reconstitution

    See Certificate of Analysis (CoA) for detailed instruction.

  • Storage
    Upon receipt, please store the beads at -20°C for 1 year in lyophilized state. Please avoid more than 3 freeze-thaw cycles once reconstitution, immediate use after reconstitution is highly recommended.
  • Assay Principles
    Antibody Purification: 1. Resuspend the lyophilized beads by adding the buffer of choice. 2. Add analyte to the suspension, mix and incubate to enable specific binding of the beads and the target protein. 3. Magnetize beads, remove supernatant, and wash unbound protein fractions to capture target protein-bound beads. 4. Wash, magnetize the beads and collect purified target protein for use in downstream applications.

    The magnetic beads technology makes use of the easy and efficient collection of beads in magnetic field to facilitate antibody purification in a simple workflow of “bind-wash-elute”. In contrast to common separation techniques, this method does not require columns or centrifugation, and is therefore ideal in high-throughput applications.

  • Formulation

    Please contact us for detailed information.

    Contact us for customized product form or formulation.

Typical Data
HGF R TYPICAL DATA

Immobilized 34.9 μg HGFR protein/1 mg beads can bind Anti-HGFR Antibody with an EC50 of 1.224 μg/mL.

Protocol
  • Clinical and Translational Updates

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재고상품 출발날짜: 영업일 기준 4일

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