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Your Position: 홈 > Protein > TNF-alpha > TNA-C52H3

Canine TNF-alpha Protein, His Tag, active trimer (MALS verified)

INTRODUCTION
QC & Assay Protocol
Citation & Background
  • Synonym
    DIF,TNF-alpha,TNFA,TNFSF2,cachexin,cachectin,TNFα
  • Source
    Canine TNF-alpha, His Tag(TNA-C52H3) is expressed from human 293 cells (HEK293). It contains AA Val 77 - Leu 233 (Accession # P51742-1).
    Predicted N-terminus: Val 77
    Request for sequence
  • Molecular Characterization
    TNF-alpha Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 18.6 kDa. The protein migrates as 19-22 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method / rFC method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
TNF-alpha SDS-PAGE

Canine TNF-alpha, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
TNF-alpha MALS images

The purity of Canine TNF-alpha, His Tag (Cat. No. TNA-C52H3) is more than 90% and the molecular weight of this protein is around 55-65 kDa verified by SEC-MALS.

Report
Bioactivity-ELISA
TNF-alpha ELISA

Immobilized Humira at 5 μg/mL (100 μL/well) can bind Canine TNF-alpha, His Tag (Cat. No. TNA-C52H3) with a linear range of 0.3-1 ng/mL (QC tested).

Protocol
TNF-alpha ELISA

Immobilized Human TNFR1, Fc Tag (Cat. No. TN1-H5251) at 5 μg/mL (100 μL/well) can bind Canine TNF-alpha, His Tag (Cat. No. TNA-C52H3) with a linear range of 2-10 ng/mL (Routinely tested).

Protocol
  • Background
    Tumor necrosis factor alpha (TNFα) is a cytokine produced primarily by monocytes and macrophages. It is found in synovial cells and macrophages in the tissues.The primary role of TNFα is in the regulation of immune cells. TNFα is able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNFα production has been implicated in a variety of human diseases, including major depression, Alzheimer's disease and cancer. Recombinant TNFα is used as an immunostimulant under the INN tasonermin. TNFα can be produced ectopically in the setting of malignancy and parallels parathyroid hormone both in causing secondary hypercalcemia and in the cancers with which excessive production is associated.
  • Clinical and Translational Updates

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