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Your Position: 홈 > Protein > CTLA-4 > CT4-H5229

Human CTLA-4 / CD152 Protein, His Tag

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INTRODUCTION
QC & Assay Protocol
Citation & Background
  • Synonym
    CTLA4,CD152
  • Source
    Human CTLA-4, His Tag (CT4-H5229) is expressed from human 293 cells (HEK293). It contains AA Ala 37 - Phe 162 (Accession # P16410-1).
    Predicted N-terminus: Ala 37
    Request for sequence
  • Molecular Characterization
    Online(Ala 37 - Phe 162) P16410-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 15.4 kDa. The protein migrates as 20-25 kDa under reducing (R) condition (SDS-PAGE) due to different extent glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 6 months under sterile conditions after reconstitution.
SDS-PAGE
Human CTLA-4, His Tag (Cat. No. CT4-H5229) SDS-PAGE gel

Human CTLA-4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human CTLA-4, His TagHuman CTLA-4, His Tag (Cat. No. CT4-H5229) ELISA bioactivity

Immobilized Human B7-1, Fc Tag (HPLC-verified) (Cat. No. B71-H5259) at 2 μg/mL (100 μL/well) can bind Human CTLA-4, His Tag (Cat. No. CT4-H5229) with a linear range of 0.2-4 ng/mL (QC tested).

Protocol
Human CTLA-4, His TagHuman CTLA-4, His Tag (Cat. No. CT4-H5229) ELISA bioactivity

Immobilized Human B7-2, Fc Tag (Cat. No. CD6-H5257) at 2 μg/mL (100 μL/well) can bind Human CTLA-4, His Tag (Cat. No. CT4-H5229) with a linear range of 1-6.4 ng/mL (Routinely tested).

Protocol
Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Yervoy (Ipilimumab, Human IgG1) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human CTLA-4, His Tag (Cat. No. CT4-H5229) with an affinity constant of 25.7 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

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