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Cynomolgus IL-31 Protein, His Tag

  • Synonym
    IL-31,IL31,Interleukin-31
  • Source
    Cynomolgus IL-31, His Tag(IL1-C5245) is expressed from human 293 cells (HEK293). It contains AA Leu 27 - Thr 163 (Accession # G7PJ78-1).
    Predicted N-terminus: His
  • Molecular Characterization
    IL-31 Structure

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 17.3 kDa. The protein migrates as 20-25 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IL-31 SDS-PAGE

Cynomolgus IL-31, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

Bioactivity-ELISA
 IL-31 ELISA

Immobilized Cynomolgus IL-31, His Tag (Cat. No. IL1-C5245) at 1 μg/mL (100 μL/well) can bind Cynomolgus/Rhesus macaque IL-31 RA Protein, Fc Tag (Cat. No. ILA-H525b) with a linear range of 5-78 ng/mL (QC tested).

  • Background
    IL-31 is an inflammatory cytokine that helps trigger cell-mediated immunity against pathogens. Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. It has also been identified as a major player in a number of chronic inflammatory diseases, including atopic dermatitis. May function in skin immunity. IL-31 is produced by a variety of cells, namely type 2 helper (TH2) T-cells. IL-31 sends signals through a receptor complex made of IL-31RA and oncostatin M receptor β (OSMRβ) expressed in immune and epithelial cells. These signals activate three pathways: ERK1/2 MAP kinase, PI3K/AKT, and JAK1/2 signaling pathways.
  • Clinical and Translational Updates

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