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Your Position: 홈 > Beads > Her2 > MBS-K006

Human HER2-coupled Magnetic Beads

ItemsSize （2mg）Size（5mg X 2）
Particle size2 μm2 μm
Physical appearancePowder mixturePowder mixture
Amount of Coupled Protein≈589 pmol (43 μg) Her2/mg Beads≈589 pmol (43 μg) Her2/mg Beads
Binding Capacity>180 pmol (27 μg) antibody/mg beads>180 pmol (27 μg) antibody/mg beads
FormulationPBS, pH7.4, with 10% TrehalosePBS, pH7.4, with 10% Trehalose
Reconstitution2 mL sterile deionized water (1 mg beads/mL)5 mL sterile deionized water (1 mg beads/mL)
  • Background
    The biotinylated HER2 protein was conjugated to streptavidin magnetic beads. This pre-coupled magnetic bead product can capture the anti-HER2 antibody from various assay systems. The beads are in uniform size, narrow size distribution with large surface area and unique surface coating, which can help you get the best performance and highly reproducible results. This HER2 coupled magnetic beads will bring great convenience with minimum non-specific binding and developed protocols. This ready-to-use product could greatly save your time and hassle.
  • Source
    Human HER2 protein is expressed from human 293 cells (HEK293). It contains AA Thr 23 - Thr 652(Accession # P04626-1).
  • Application
    This product is intended for immunocapture, biopanning and flow cytometry. This product is produced non-sterile.
    *The Isotype control (Cat. No. SMB-B01) is sold separately.
  • Reconstitution

    See Certificate of Analysis (CoA) for detailed instruction.

  • Storage
    Upon receipt, please store the Beads at -20°C. The shelf life is 1 year at -20 °C. Once reconstitution, please aliquote and store under -70°C for no more than 3 months, and avoid repeated freeze-thaw cycles. Immediate use after reconstitution is highly recommended.Please avoid more than 3 freeze-thaw cycles once reconstitution, immediate use after reconstitution is highly recommended.
  • Assay Principles
    Antibody Purification: 1. Resuspend the lyophilized beads by adding the buffer of choice. 2. Add analyte to the suspension, mix and incubate to enable specific binding of the beads and the target protein. 3. Magnetize beads, remove supernatant, and wash unbound protein fractions to capture target protein-bound beads. 4. Wash, magnetize the beads and collect purified target protein for use in downstream applications.

    The magnetic beads technology makes use of the easy and efficient collection of beads in magnetic field to facilitate antibody purification in a simple workflow of “bind-wash-elute”. In contrast to common separation techniques, this method does not require columns or centrifugation, and is therefore ideal in high-throughput applications.

  • Formulation

    Please contact us for detailed information.

    Contact us for customized product form or formulation.

Typical Data
Her2 TYPICAL DATA

Immobilized 43 μg HER2 protein to 1 mg Beads, can bind the Anti-HER2 Antibody (Herceptin) with an EC50 of 0.7817 μg/mL.

Protocol
  • Clinical and Translational Updates

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