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Your Position: > Protein > LRP-6 > LR6-H5253

Human LRP-6 (20-630) Protein, Mouse IgG2a Fc Tag

  • Synonym
    LRP-6,LRP6
  • Source
    Human LRP-6 (20-630), Mouse IgG2a Fc Tag(LR6-H5253) is expressed from human 293 cells (HEK293). It contains AA Ala 20 - Pro 630 (Accession # O75581-1  ).
    Predicted N-terminus: Ala 20
  • Molecular Characterization
    LRP-6 Structure

    This protein carries a mouse IgG2a Fc tag at the C-terminus.

    The protein has a calculated MW of 95.8 kDa. The protein migrates as 55 kDa and 110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
LRP-6 SDS-PAGE

Human LRP-6 (20-630), Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
 LRP-6 ELISA

Immobilized Mouse Dkk-1, His Tag (Cat. No. DK1-M52H6) at 5 μg/mL (100 μL/well) can bind Human LRP-6 (20-630), Mouse IgG2a Fc Tag (Cat. No. LR6-H5253) with a linear range of 10-78 ng/mL (QC tested).

 LRP-6 ELISA

Immobilized Human R-Spondin 1, His Tag (Cat. No. RS1-H4221) at 5 μg/mL (100 μL/well) can bind Human LRP-6 (20-630), Mouse IgG2a Fc Tag (Cat. No. LR6-H5253) with a linear range of 1-20 ng/mL (Routinely tested).

 LRP-6 ELISA

Immobilized Human LRP-6 (20-630), Mouse IgG2a Fc Tag (Cat. No. LR6-H5253) at 5 μg/mL (100 μL/well) can bind Biotinylated Human SOST, His Tag, primary amine labeling (Cat. No. SOT-H8245) with a linear range of 0.005-0.313 μg/mL (Routinely tested).

  • Background
    LRP6 is a transmembrane low-density lipoprotein receptor that shares a similar structure with LRP5. Low-density lipoprotein receptor-related protein 6 is a key component of the LRP5/LRP6/Frizzled co-receptor group that is involved in canonical Wnt pathway. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalsomes and inhibiting AXIN1/GSK3-mediated phosphorylation and destruction of beta-catenin.
  • Clinical and Translational Updates

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