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Your Position: > Protein > IL-33 > IL3-H52H7

Human IL-33 Protein, His Tag, premium grade

  • Synonym
    IL33,DV27,C9ORF26,IL1F11,NFHEV,DKFZp586H0523,DVS27,NFEHEV,RP11-575C20.2
  • Source
    Human IL-33 Protein, His Tag, premium grade(IL3-H52H7) is expressed from human 293 cells (HEK293). It contains AA His 109 - Thr 270 (Accession # O95760-1).
    Predicted N-terminus: His
    Human IL-33 Protein, His Tag, premium grade (IL3-H52H7), designed for preclinical stage, has the same activity and performance with GMP Human IL-33 Protein, which enables a seamless transition from preclinical development to clinical phases. Premium Grade product offer a cost efficient alternative of GMP Grade products for the early development phase when safety of raw materials is not top priority. By using Premium Grade products in early development phase, you can transition easily into clinical and commercial phase without need to revalidate the raw materials and modify manufacturing process.
  • Molecular Characterization
    IL-33 Structure

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 20.2 kDa. The protein migrates as 25-30 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Sterility
    The sterility testing was performed by membrane filtration method.
  • Mycoplasma
    Negative.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IL-33 SDS-PAGE

Human IL-33 Protein, His Tag, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
IL-33 MALS images

The purity of Human IL-33 Protein, His Tag, premium grade (Cat. No. IL3-H52H7) is more than 85% and the molecular weight of this protein is around 18-26 kDa verified by SEC-MALS.

Bioactivity-Bioactivity CELL BASE
 IL-33 CELL

Human IL-33 Protein, His Tag, premium grade (Cat. No. IL3-H52H7) induces IL-8 secretion in HUVECs. The EC50 for this effect is 193.0-311.8 ng/mL (Routinely tested).

Bioactivity-ELISA
 IL-33 ELISA

Immobilized Human IL-1RL1, Fc Tag (Cat. No. IL1-H5250) at 5 μg/mL (100 μL/well) can bind Human IL-33 Protein, His Tag, premium grade (Cat. No. IL3-H52H7) with a linear range of 5-40 ng/mL (QC tested).

Bioactivity-BLI
 IL-33 BLI

Loaded Human IL-1RL1, Fc Tag (Cat. No. IL1-H5250) on Protein A Biosensor, can bind with Human IL-33 Protein, His Tag, premium grade (Cat. No. IL3-H52H7) an affinity constant of 1.31 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    Interleukin 33 (IL33) is known as C9orf26, DKFZp586H0523, DVS27, NF-HEV, NFEHEV, RP11-575C20.2,and is a cytokine belonging to the IL-1 superfamily. IL-33 induces helper T cells, mast cells, eosinophils and basophils to produce type 2 cytokines. IL-33 mediates its biological effects by interacting with the receptors ST2 (aka IL1RL1) and IL-1 Receptor Accessory Protein (IL1RAP), activating intracellular molecules in the NF-κB and MAP kinase signaling pathways that drive production of type 2 cytokines (e.g. IL-5 and IL-13) from polarized Th2 cells. In vivo, IL-33 induces the expression of IL-4, IL-5, and IL-13 and leads to severe pathological changes in mucosal organs.
  • Clinical and Translational Updates

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재고상품 출발날짜: 영업일 기준 4일

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  • Latest Research Phase:Phase 3 Clinical

Datasheet & Documentation

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DMF (Drug Master File)

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