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Your Position: > Protein > PVRIG > PVG-C5259

Cynomolgus PVRIG Protein, Fc Tag

  • 동의어
    C7orf15,C7orf15MGC138295,CD112R,MGC104322,MGC138297,MGC2463,PVRIG,CD112 receptor
  • 출처
    Cynomolgus PVRIG, Fc Tag (PVG-C5259) is expressed from human 293 cells (HEK293). It contains AA Thr 41 - Asp 171 (Accession # A0A2K5WVV8-1).
    Predicted N-terminus: Thr 41
  • 분자 표징
    Online(Thr 41 - Asp 171) A0A2K5WVV8-1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 40.1 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 내독소
    Less than 1.0 EU per μg by the LAL method.
  • 순도

    >95% as determined by SDS-PAGE.

  • 레시피

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • 재구성

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 보관

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Cynomolgus PVRIG, Fc Tag (Cat. No. PVG-C5259) SDS-PAGE gel

Cynomolgus PVRIG, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Cynomolgus PVRIG, Fc TagCynomolgus PVRIG, Fc Tag (Cat. No. PVG-C5259) ELISA bioactivity

Immobilized Human Nectin-2, Mouse IgG2a Fc Tag (Cat. No. CD2-H5257) at 1 μg/mL (100 μL/well) can bind Cynomolgus PVRIG, Fc Tag (Cat. No. PVG-C5259) with a linear range of 0.5-8 ng/mL (QC tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Cynomolgus PVRIG, Fc Tag (Cat. No. PVG-C5259) on Protein A Biosensor, can bind Human Nectin-2, His Tag (Cat. No. PV2-H52E2) with an affinity constant of 0.44 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • 백그라운드
    Human PVRIG (poliovirus receptor related immunoglobulin domain-containing protein), also known as CD112 receptor (CD112R), is an approximately 34 kDa single transmembrane protein in the poliovirus receptor-like protein (PVR) family. The CD112R gene encodes a putative single transmembrane protein, which is composed of a single extracellular IgV domain, one transmembrane domain, and a long intracellular domain. Notably, the intracellular domain of phatases. The extracellular domain sequence of human and mouse CD112R have 65.3% similarity. CD112R may act as a coinhibitory receptor that suppresses T-cell receptor-mediated signals.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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