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ActiveMax® Human TNF-alpha Protein, Tag Free, low endotoxin (active trimer) (MALS verified)

  • Synonym
    DIF,TNF-alpha,TNFA,TNFSF2,cachexin,cachectin,TNFα
  • Source
    ActiveMax® Human TNF-alpha, Tag Free (TNA-H4211) is expressed from human 293 cells (HEK293). It contains AA Val 77 - Leu 233 (Accession # P01375-1).
    Predicted N-terminus: Val 77
  • Molecular Characterization
    Online(Val 77 - Leu 233) P01375-1

    This protein carries no "tag".

    The protein has a calculated MW of 17.4 kDa. The protein migrates as 17 kDa under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Sterility

    The sterility testing was performed by membrane filtration method described in USP<71>and Eur. Ph. 2.6.1.

  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
ActiveMax® Human TNF-alpha, Tag Free (Cat. No. TNA-H4211) SDS-PAGE gel

ActiveMax® Human TNF-alpha, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
ActiveMax® Human TNF-alpha, Tag Free (Cat. No. ) MALS images

The purity of ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (active trimer) (MALS verified) (Cat. No. TNA-H4211) is more than 95% in HP-SEC, and the molecular weight of this protein is around 47-60 kDa verified by SEC-MALS.

Bioactivity-ELISA
ActiveMax® Human TNF-alpha, Tag FreeActiveMax® Human TNF-alpha, Tag Free (Cat. No. TNA-H4211) ELISA bioactivity

Immobilized ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (active trimer) (MALS verified) (Cat. No. TNA-H4211) at 2 μg/mL (100 μL/well) can bind Humira with a linear range of 0.2-2 ng/mL (QC tested).

ActiveMax® Human TNF-alpha, Tag FreeActiveMax® Human TNF-alpha, Tag Free (Cat. No. TNA-H4211) ELISA bioactivity

Immobilized ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (active trimer) (MALS verified) (Cat. No. TNA-H4211) at 2 μg/mL (100 μL/well) can bind Human TNFR1, Fc Tag (Cat. No. TN1-H5251) with a linear range of 0.2-3 ng/mL (Routinely tested).

ActiveMax® Human TNF-alpha, Tag FreeActiveMax® Human TNF-alpha, Tag Free (Cat. No. TNA-H4211) ELISA bioactivity

Immobilized ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (Cat. No. TNA-H4211) at 5 μg/mL (100 μL/well) can bind Human TNFR2, His Tag (Cat. No. TN2-H5227) with a linear range of 5-39 ng/mL (Routinely tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Humira (Adalimumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (active trimer) (MALS verified) (Cat. No. TNA-H4211) with an affinity constant of 0.255 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Bioactivity-Cell based assay
Human FcRn Heterodimer Protein Cell_Base

ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (active trimer) (MALS verified) (Cat. No. TNA-H4211) induces cytotoxicity effect on the WEH1-13VAR cells in the presence of the metabolic inhibitor actinomycin D. The EC50 for this effect is 0.007-0.014 ng/mL (Routinely tested).

Human FcRn Heterodimer Protein Cell_Base

Neutralization assay shows that the cytotoxicity effect of ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (active trimer) (MALS verified) (Cat. No. TNA-H4211) was inhibited by increasing concentration of Adalimumab. The concentration of TNF-alpha used is 1 ng/mL. The IC50 is 29 ng/mL (Routinely tested).

Human FcRn Heterodimer Protein Cell_Base

The NF-kB (Luc) HEK293 Reporter Cell (Cat. No. CHEK-ATF048) was stimulated with serial dilutions of ActiveMax® Human TNF-alpha, Tag Free (Cat. No. TNA-H4211). The max induction fold was approximately 235 (Routinely tested).

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ETD of in-stock products: 4 business days

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가격(KRW) : 256,500

가격(KRW) : 1,566,000

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