로그인 회원가입
icon_bulk_order대량 주문 Acrobiosystems for English
Promotion
Recombinant Proteins ClearClear history
Recombinant Proteins CloseClose
Browse Products By Molecule Name a-zA-Z
TL1A Hot |GMP IL-2 Hot |FcRn Binding Kit (TR-FRET) New |Claudin 18.2 Hot
0
장바구니가 비어 있습니다
내 장바구니
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: 홈 > Protein > TNF-alpha > TNA-H4211

Human TNF-alpha Protein, premium grade

GMP version GMP-TNAH23 is now available for seamless transition.
INTRODUCTION
QC & Assay Protocol
Citation & Background
  • Synonym
    DIF,TNF-alpha,TNFA,TNFSF2,cachexin,cachectin,TNFα
  • Source
    Human TNF-alpha, premium grade(TNA-H4211) is expressed from human 293 cells (HEK293). It contains AA Val 77 - Leu 233 (Accession # P01375-1).
    Predicted N-terminus: Val 77
    It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.
    Request for sequence
  • Molecular Characterization
    TNF-alpha Structure

    This protein carries no "tag".

    The protein has a calculated MW of 17.4 kDa. The protein migrates as 17 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.01 EU per μg by the LAL method.
  • Host Cell Protein
    <0.5 ng/µg of protein tested by ELISA.
  • Host Cell DNA
    <0.02 ng/μg of protein tested by qPCR.
  • Sterility
    Negative
  • Mycoplasma
    Negative.
  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 24 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
TNF-alpha SDS-PAGE

Human TNF-alpha, premium grade on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
TNF-alpha MALS images

The purity of Human TNF-alpha, premium grade (Cat. No. TNA-H4211) is more than 95% and the molecular weight of this protein is around 52-62 kDa verified by SEC-MALS.

Report
Bioactivity-Bioactivity CELL BASE
TNF-alpha CELL

Human TNF-alpha, premium grade (Cat. No. TNA-H4211) induces cytotoxicity effect on the WEHI-13VAR cells in the presence of the metabolic inhibitor actinomycin D. The specific activity of Human TNF-alpha, premium grade is >2.00×10^7 IU/mg, which is calibrated against human TNF-alpha WHO International Standard (NIBSC code: 12/154) (QC tested).

Protocol
TNF-alpha CELL

Neutralization assay shows that the cytotoxicity effect of human TNF-alpha, premium grade (Cat. No. TNA-H4211) was inhibited by increasing concentration of Adalimumab. The concentration of TNF-alpha used is 20 pg/mL. The IC50 is 8.48 ng/mL (Routinely tested).

Protocol
TNF-alpha CELL

The NF-kB (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of Human TNF-alpha, premium grade (Cat. No. TNA-H4211). The EC50 value of this effect is 0.5143 ng/mL (Routinely tested).

Protocol
Bioactivity-ELISA
TNF-alpha ELISA

Immobilized Human TNF-alpha, premium grade (Cat. No. TNA-H4211) at 1 μg/mL (100 μL/well) can bind Humira with a linear range of 0.1-2 ng/mL (QC tested).

Protocol
TNF-alpha ELISA

Immobilized Human TNF-alpha, premium grade (Cat. No. TNA-H4211) at 2 μg/mL (100 μL/well) can bind Human TNFR1, Fc Tag (Cat. No. TN1-H5251) with a linear range of 0.1-4 ng/mL (Routinely tested).

Protocol
TNF-alpha ELISA

Immobilized Human TNF-alpha, premium grade (Cat. No. TNA-H4211) at 5 μg/mL (100 μL/well) can bind Human TNFR2, His Tag (Cat. No. TN2-H5227) with a linear range of 0.039-0.313 μg/mL (Routinely tested).

Protocol
Bioactivity-SPR
TNF-alpha SPR

Monoclonal Anti-TNF-alpha Antibody, Human IgG1 (13B8) (Cat. No. TNA-AM493) captured on Protein A Chip can bind Human TNF-alpha, premium grade (Cat. No. TNA-H4211) with an affinity constant of 0.267 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Protocol
Bioactivity-Stability
TNF-alpha STABILITY

The Cell based assay shows batch-to-batch consistency between Acro's GMP and PG TNF-alpha.

  • Background
    Tumor necrosis factor alpha (TNFα) is a cytokine produced primarily by monocytes and macrophages. It is found in synovial cells and macrophages in the tissues.The primary role of TNFα is in the regulation of immune cells. TNFα is able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNFα production has been implicated in a variety of human diseases, including major depression, Alzheimer's disease and cancer. Recombinant TNFα is used as an immunostimulant under the INN tasonermin. TNFα can be produced ectopically in the setting of malignancy and parallels parathyroid hormone both in causing secondary hypercalcemia and in the cancers with which excessive production is associated.
  • Clinical and Translational Updates

코멘트 (0)


재고상품 출발날짜: 영업일 기준 4일

가격(KRW) : 102,000

가격(KRW) : 285,000

가격(KRW) : 1,740,000

장바구니에 담기 대량구매 맞춤형 제품
Request GMP Version

Antibody Licensing

more

보급 및 전시



약물 개발 현황

  • Number of Launched Drugs：61 Details
  • Number of Drugs in Clinical Trials：63 Details
  • Latest Research Phase：Approved
다른 추천 제품

Datasheet & Documentation

DMF 신청
DMF (Drug Master File)

연락처

+1  800-810-0816 (US)
+86 400-682-2521 (AP)
Show All Distributors

관련 제품

신제품

문의와 컨텐츠

This web search service is supported by Google Inc.

totop
Call us
우리에게 전화하세요
글로벌: +1 800-810-0816 (무료)
아시아태평양: +86 400-682-2521
Email
이메일
order.kr@acrobiosystems.com
Fax
팩스
+1 888-377-6111
Address
주소
서울 성동구 왕십리로10길 6 서울숲비즈포레 703
회사명
아크로바이오 주식회사
사업자 등록번호
186-88-02858
대표자
CHEN YIDING
사업장주소
서울특별시 송파구 중대로12길 10-20, 1층 셀렉티드송파(가락동)
linkedin twitter facebook youtube

문의

Copyright © 2017 ACROBiosystems. All Rights Reserved
Related Links|Privacy Policy|Terms and Conditions|Legal|Sitemap