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Human CD27 / TNFRSF7 Protein, His Tag (MALS Verified)

  • 동의어
    CD27,TNFRSF7,S152,T14,Tp55
  • 출처
    Human CD27, His Tag (CD7-H522b) is expressed from human 293 cells (HEK293). It contains AA Thr 21 - Ile 192 (Accession # P26842-1).
    Predicted N-terminus: Thr 21
  • 분자 표징
    Online(Thr 21 - Ile 192) P26842-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 21.1 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 내독소
    Less than 1.0 EU per μg by the LAL method.
  • 순도

    >90% as determined by SDS-PAGE.

  • 레시피

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • 재구성

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 보관

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human CD27, His Tag (Cat. No. CD7-H522b) SDS-PAGE gel

Human CD27, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Human CD27, His Tag (Cat. No. ) MALS images

The purity of Human CD27, His Tag (Cat. No. CD7-H522b) is more than 85% and the molecular weight of this protein is around 30-45 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human CD27, His TagHuman CD27, His Tag (Cat. No. CD7-H522b) ELISA bioactivity

Immobilized Human CD27, His Tag (Cat. No. CD7-H522b) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD27 Ligand, Avitag,Fc Tag (Cat. No. TN7-H82F4) with a linear range of 2-31 ng/mL (QC tested).

  • 백그라운드
    CD Antigen CD27 is also known as Tumor necrosis factor receptor superfamily member 7 (TNFRSF7), which belongs to TNF-receptor superfamily. CD27 / TNFRSF7 is found in most T-lymphocytes. CD27 / TNFRSF7 is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-κB and MAPK8/JNK. CD27 has been shown to interact with SIVA1, TRAF2 and TRAF3.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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