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Your Position: 홈 > Protein > B7-1 > B71-H82E9

Biotinylated Human B7-1 / CD80 Protein, Avitag™,His Tag (recommended for biopanning)

소개
QC & Assay Protocol
Citation & 백그라운드
  • 동의어
    CD80,B7,B7-1,B7.1,BB1,CD28LG,CD28LG1,LAB7
  • 출처
    Biotinylated Human B7-1 Protein, Avitag,His Tag (B71-H82E9) is expressed from human 293 cells (HEK293). It contains AA Val 35 - Asn 242 (Accession # P33681-1).
    Predicted N-terminus: Val 35
    Request for sequence
  • 분자 표징
    Online(Val 35 - Asn 242) P33681-1

    This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.

    The protein has a calculated MW of 26.5 kDa. The protein migrates as 44-54 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 어플리케이션
    B71-H82E9 works best for experiments that test the binding between B7-1 and candidate antibodies, such as biopanning and other relevant assays.
    This product is NOT suitable for neither B7-1 - CTLA-4 or B7-1 - CD28 binding test. For this type of application, we strongly recommend you to choose Biotinylated Human B7-1 Protein, Avitag,His Tag (B71-H82F2) as an alternative.
  • 비오틴화
    비오틴화 of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • 내독소
    Less than 1.0 EU per μg by the LAL method.
  • 순도

    >95% as determined by SDS-PAGE.

  • 레시피

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • 재구성

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 보관

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human B7-1 Protein, Avitag,His Tag (Cat. No. B71-H82E9) SDS-PAGE gel

Biotinylated Human B7-1 Protein, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated Human B7-1 Protein, Avitag,His TagBiotinylated Human B7-1 Protein, Avitag,His Tag (Cat. No. B71-H82E9) ELISA bioactivity

Immobilized Anti-CD80 MAb, Human IgG1 at 5 μg/mL (100 μL/well) can bind Biotinylated Human B7-1 Protein, Avitag,His Tag (Cat. No. B71-H82E9) with a linear range of 0.1-3 ng/mL (QC tested).

Protocol
  • 백그라운드
    B7-1 and B7-2, together with their receptors CD28 and CTLA­4, constitute one of the dominant co-stimulatory pathways that regulate T­ and B­cell responses. Although both CTLA­4 and CD28 can bind to the same ligands, CTLA­4 binds to B7­1 and B7­2 with a 20 ­ 100 fold higher affinity than CD28 and is involved in the down­regulation of the immune response. B-lymphocyte activation antigen B7-1 (referred to as B7) also known as cluster of Differentiation 80 (CD80), is a member of cell surface immunoglobulin superfamily and is expressed on activated B cells, activated T cells, macrophages and dendritic cells. It is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. CD80 plays a role in induction of innate immune responses by activating NF-κB-signaling pathway in macrophages. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.
  • Clinical and Translational Updates
      

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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