로그인 회원가입
icon_bulk_order대량 주문 Acrobiosystems for English
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: > CAR-T > BCMA > BC7-H82F0

Biotinylated Human BCMA / TNFRSF17 Protein, Fc,Avitag™ DMF Filed

인기 제품 추천: SP-11 스트렙타비딘 코팅된 96웰 플레이트  샘플 신청 
STN-N5116 스트렙타비딘 단백질  SMB-B01 스트렙타비딘 마그네틱 비드 
비오틴-스트렙타비딘 시스템의 높은 특이성과 고감도를 이용하여 비오틴화된 단백질을 스트렙타비딘 관련 제품과 결합하여 다양한 실험에서 사용할 수 있습니다. 비비오틴 시스템과 비교하면 검출이 더 빠르고 더 강력하고 더 낮은 비특이적 배경을 가지고 있습니다.
  • 동의어
    TNFRSF17,CD269,BCM,BCMA
  • 출처
    Biotinylated Human BCMA, Fc,Avitag (BC7-H82F0) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 54 (Accession # NP_001183.2).
    Predicted N-terminus: Met 1
  • 분자 표징
    Online(Met 1 - Ala 54) NP_001183.2

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 33.8 kDa. The protein migrates as 40-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 비오틴화
    비오틴화 of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • 내독소
    Less than 1.0 EU per μg by the LAL method.
  • 순도

    >95% as determined by SDS-PAGE.

  • 레시피

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • 재구성

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 보관

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human BCMA, Fc,Avitag (Cat. No. BC7-H82F0) SDS-PAGE gel

Biotinylated Human BCMA, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated Human BCMA, Fc,AvitagBiotinylated Human BCMA, Fc,Avitag (Cat. No. BC7-H82F0) ELISA bioactivity

Immobilized Human BAFF, Fc Tag, active trimer (Cat. No. BAF-H5261) at 2 μg/mL (100 μL/well) can bind Biotinylated Human BCMA, Fc,Avitag (Cat. No. BC7-H82F0) with a linear range of 0.1-2 ng/mL (QC tested).

Biotinylated Human BCMA, Fc,AvitagBiotinylated Human BCMA, Fc,Avitag (Cat. No. BC7-H82F0) ELISA bioactivity

Immobilized Anti-Human BCMA Mab at 2 μg/mL (100 μL/well) can bind Biotinylated Human BCMA, Fc,Avitag (Cat. No. BC7-H82F0) with a linear range of 0.1-3 ng/mL (Routinely tested).

Biotinylated Human BCMA, Fc,AvitagBiotinylated Human BCMA, Fc,Avitag (Cat. No. BC7-H82F0) ELISA bioactivity

Quantitative Analysis of Bispecific T-cell Engager (CD3 X BCMA) in human serum.
Immobilized Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free (Cat. No. CDD-H52W1) at 2 μg/mL, add increasing concentrations of Bispecific T cell Engager (CD3 X BCMA) in 10% human serum and then add Biotinylated BCMA Fc,Avitag (Cat. No. BC7-H82F0) at 0.2 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 4 ng/mL (Routinely tested).

Biotinylated Human BCMA, Fc,AvitagBiotinylated Human BCMA, Fc,Avitag (Cat. No. BC7-H82F0) ELISA bioactivity

Immobilized Human APRIL, His,Flag Tag, active trimer (Cat. No. APL-H52D1) at 1 μg/mL (100 μL/well) can bind Biotinylated Human BCMA, Fc,Avitag (Cat. No. BC7-H82F0) with a linear range of 0.1-2 ng/mL (Routinely tested).

Bioactivity-FACS
FACS assay shows that recombinant Biotinylated FACS

2e5 of anti-BCMA CAR-293 cells were stained with 100 μL of 3 μg/mL of Biotinylated Human BCMA, Fc,Avitag (Cat. No. BC7-H82F0) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (QC tested).

Evaluation of CAR expression
FACS analysis of anti-BCMA CAR expression
Evaluation of CAR expression

Human T cells were transfected with anti-BCMA CAR and cultured for 3 days. Three days post-transfection, 1e6 cells were first incubated with 50 μL Biotinylated Human BCMA, Fc,Avitag (Cat. No. BC7-H82F0, 8 μg/mL), washed and then stained with PE Streptavidin and analyzed by flow cytometry. (Data are kindly provided by PREGENE Biopharma)

코멘트 (0)


ETD of in-stock products: 4 business days

가격(KRW) : 375,000

가격(KRW) : 1,620,000

Produit en vogue recommandé
스트렙타비딘 코팅판

가격(KRW) : 45,000

가격(KRW) : 195,000

스트렙타비딘-HRP

가격(KRW) : 36,000

가격(KRW) : 270,000

Magnetic Beads™ 스트렙타비딘

가격(KRW) : 315,000

가격(KRW) : 525,000

보급 및 전시



약물 개발 현황

  • Number of Launched Drugs:2 Details
  • Number of Drugs in Clinical Trials:58 Details
  • Latest Research Phase:Approved

데이터 테이블 및 파일

DMF 신청
DMF (Drug Master File)

연락처

+1  800-810-0816 (US)
+86 400-682-2521 (AP)

신제품

의문 과 코맨트

This web search service is supported by Google Inc.

totop