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Your Position: 홈 > ADC > TNF-alpha > TNA-H82E3

Biotinylated Human TNF-alpha Protein, His,Avitag™ (active trimer) (MALS verified)

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소개
QC & Assay Protocol
Citation & 백그라운드
  • 동의어
    DIF,TNF-alpha,TNFA,TNFSF2,cachexin,cachectin,TNFα
  • 출처
    MABSol® Biotinylated Human TNF-alpha, His,Avitag (active trimer) (MALS verified) (TNA-H82E3) is expressed from human HEK293 cells. It contains AA Val 77 - Leu 233 (Accession # NP_000585.2).
    Predicted N-terminus: Val 77
    Request for sequence
  • 분자 표징
    Online(Val 77 - Leu 233) NP_000585.2

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 20.0 kDa. The protein migrates as 21 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 비오틴화
    비오틴화 of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • 내독소
    Less than 1.0 EU per μg by the LAL method.
  • 순도

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • 레시피

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • 재구성

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 보관

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 12 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human TNF-alpha, His,Avitag (active trimer) (MALS verified) (Cat. No. TNA-H82E3) SDS-PAGE gel

Biotinylated Human TNF-alpha, His,Avitag (active trimer) (MALS verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated Human TNF-alpha, His,Avitag (active trimer) (MALS verified) (Cat. No. ) MALS images

The purity of Biotinylated Human TNF-alpha, His,Avitag (active trimer) (MALS verified) (Cat. No. TNA-H82E3) is more than 90% in HP-SEC, and the molecular weight of this protein is around 55-70 kDa verified by SEC-MALS.

Report
Bioactivity-ELISA
Biotinylated Human TNF-alpha, His,Avitag (active trimer) (MALS verified)Biotinylated Human TNF-alpha, His,Avitag (active trimer) (MALS verified) (Cat. No. TNA-H82E3) ELISA bioactivity

Immobilized Humira at 5 μg/mL (100 μL/well) can bind Biotinylated Human TNF-alpha, His,Avitag (active trimer) (MALS verified) (Cat. No. TNA-H82E3) with a linear range of 0.4-6 ng/mL (QC tested).

Protocol
Biotinylated Human TNF-alpha, His,Avitag (active trimer) (MALS verified)Biotinylated Human TNF-alpha, His,Avitag (active trimer) (MALS verified) (Cat. No. TNA-H82E3) ELISA bioactivity

Immobilized Human TNFR2, His Tag (Cat. No. TN2-H5227) at 5 μg/mL (100 μL/well) can bind Biotinylated Human TNF-alpha, His,Avitag (active trimer) (MALS verified) (Cat. No. TNA-H82E3) with a linear range of 0.6-10 ng/mL (Routinely tested).

Protocol
Biotinylated Human TNF-alpha, His,Avitag (active trimer) (MALS verified)Biotinylated Human TNF-alpha, His,Avitag (active trimer) (MALS verified) (Cat. No. TNA-H82E3) ELISA bioactivity

Immobilized Human TNFR1, Fc Tag (Cat. No. TN1-H5251) at 5 μg/mL (100 μL/well) can bind Biotinylated Human TNF-alpha, His,Avitag (active trimer) (MALS verified) (Cat. No. TNA-H82E3) with a linear range of 0.2-5 ng/mL (Routinely tested).

Protocol
Bioactivity-Cell based assay
Human FcRn Heterodimer Protein Cell_Base

Biotinylated Human TNF-alpha, His,Avitag (active trimer) (MALS verified) (Cat.No. TNA-H82E3) induces cytotoxicity effect on the WEH1-13VAR cells in the presence of the metabolic inhibitor actinomycin D. The EC50 for this effect is 0.029-0.052 ng/mL (Routinely tested).

Protocol
Human FcRn Heterodimer Protein Cell_Base

Neutralization assay shows that the cytotoxicity effect of Biotinylated Human TNF-alpha, His,Avitag (active trimer) (MALS verified) (Cat. No. TNA-H82E3) was inhibited by increasing concentration of Adalimumab. The concentration of TNF-alpha used is 1 ng/mL. The IC50 is 7 ng/mL (Routinely tested).

Protocol

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