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Your Position: > Protein > TFPI > TFI-H8226

Biotinylated Human TFPI Protein, His Tag, ultra sensitivity (primary amine labeling)

  • 동의어
    TFPI,LACI,TFPI1,EPI,TFI
  • 출처
    MABSol® Biotinylated Human TFPI, His Tag, primary amine labeling (TFI-H8226) is expressed from human HEK293 cells. It contains AA Asp 29 - Lys 282 (Accession # NP_006278.1). It is the biotinylated form of Human TFPI, His Tag (TFI-H5226).
    Predicted N-terminus: Asp 29
  • 분자 표징
    Online(Asp 29 - Lys 282) NP_006278.1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 30.0 kDa. The protein migrates as 41-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 비오틴화
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • 내독소
    Less than 1.0 EU per μg by the LAL method.
  • 순도

    >95% as determined by SDS-PAGE.

  • 레시피

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • 재구성

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 보관

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human TFPI, His Tag, primary amine labeling (Cat. No. TFI-H8226) SDS-PAGE gel

Biotinylated Human TFPI, His Tag, primary amine labeling on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • 백그라운드
    Tissue factor pathway inhibitor (TFPI) is also known as Extrinsic pathway inhibitor (EPI), Lipoprotein - associated coagulation inhibitor (LACI), is a plasma proteinase inhibitor synthesized by vascular endothelial cells and part of it is associated with glycosaminoglycans of these cells. TFPI is a single-chain polypeptide which can reversibly inhibit Factor Xa (Xa) and Thrombin (Factor IIa). TFPI is a secreted protein with a N­terminal acidic region, three Kunitz (K) domains separated with by two linker regions, and a C­terminal basic region. The first K domain inhibits coagulation factor VIIa complexed to tissue factor (TF); The second K domain inhibits factor Xa; The third K domain binds to heparin; The C­terminal basic region may have several functions. For example, it plays an important role in binding of TFPI to cell surfaces.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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약물 개발 현황

  • Number of Launched Drugs:1 Details
  • Number of Drugs in Clinical Trials:4 Details
  • Latest Research Phase:Approved

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