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Ask Us Anything About Our CD3 Products
Release time: 2022-08-18 Source: ACROBiosystems Read: 1568

As the bispecific antibody (bsAb) technology platform gradually develops and matures, the exploration of target combination mechanisms has become one of the core aspects of future competition. Current bsAb projects are focused on combinations of tumor surface targets and CD3, which is expressed on the surface of T cells, helping mobilize T cells to target tumor cells.

Bispecific antibody

ACROBiosystems provides a series of high-quality CD3 proteins with multiple subtypes, tags, and species to assist in bsAb drug development.

Product List
  • CD3E & CD3D

  • CD3E & CD3G

  • CD3 epsilon

  • CD3 delta

  • CD3 gamma

MoleculeCat. No.HostProduct DescriptionStructure
CD3E & CD3DCDD-H5255HEK293Human CD3 epsilon&CD3 delta Heterodimer Protein, Fc Tag&Fc Tag (MALS verified)New
CDD-H52WaHEK293Human CD3 epsilon&CD3 delta Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (MALS verified)Hot
CDD-H82W6HEK293Biotinylated Human CD3 epsilon&CD3 delta Heterodimer Protein, His,&Tag Free (MALS verified)Hot
CDD-H52W1HEK293Human CD3 epsilon & CD3 delta Heterodimer Protein, His Tag&Tag Free (MALS verified)Hot
CDD-H5258HEK293Human CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc, low endotoxin
CDD-H82F6HEK293Biotinylated Human CD3 epsilon&CD3 delta Heterodimer Protein, Fc,His,&Fc,Flag,Avitag™ (MALS verified)
CDD-H82W0HEK293Biotinylated Human CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag, ultra sensitivity (primary amine labeling) (MALS verified)
CDD-C5259HEK293Cynomolgus CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc, low endotoxin
CDD-C52W4HEK293Cynomolgus CD3 epsilon&CD3 delta Heterodimer Protein, His Tag&Flag Tag (MALS verified)
CDD-C52W9HEK293Cynomolgus CD3 epsilon&CD3 delta Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (MALS verified)
CDD-C82W6HEK293Biotinylated Cynomolgus CD3E&CD3D Heterodimer Protein, His,&Tag Free (MALS verified)
CDD-M5219HEK293Mouse CD3 epsilon&CD3 delta Heterodimer Protein, His Tag&Flag Tag (MALS verified)
CDD-M52W2HEK293Mouse CD3 epsilon&CD3 delta Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (MALS verified)
CDD-M82W5HEK293Biotinylated Mouse CD3 epsilon&CD3 delta Heterodimer Protein, His,&Tag Free
CDD-R5257HEK293Rat CD3 epsilon&CD3 delta Heterodimer Protein, Llama Fc Tag&Llama Fc Tag (MALS verified)
CDD-R52D8HEK293Rat CD3 epsilon&CD3 delta Heterodimer Protein, His Tag&Flag Tag (MALS verified)
CDD-R5259HEK293Rabbit CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc, low endotoxin (MALS verified)
CDD-R52D7HEK293Rabbit CD3 epsilon&CD3 delta Heterodimer Protein, His Tag&Flag Tag (MALS verified)
MBS-K003
Human CD3E & CD3D Heterodimer-coupled Magnetic Beads
MoleculeCat. No.HostProduct DescriptionStructure
CD3E & CD3GCDG-H5253HEK293Human CD3 epsilon&CD3 gamma Heterodimer Protein, Fc Tag&Fc Tag (MALS verified)New
CDG-H52W9HEK293Human CD3 epsilon&CD3 gamma Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (MALS verified)Hot
CDG-H52W5HEK293Human CD3 epsilon&CD3 gamma Heterodimer Protein, His Tag&Tag Free (MALS verified)
CDG-H82W3HEK293Biotinylated Human CD3 epsilon&CD3 gamma Heterodimer Protein, His,&Tag Free (MALS verified)
CDG-C5257HEK293Cynomolgus CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag (MALS verified)
CDG-C52W6HEK293Cynomolgus CD3E&CD3G Heterodimer Protein, His Tag&Tag Free (MALS verified)
CDG-C8219HEK293Biotinylated Cynomolgus CD3E&CD3G Heterodimer Protein, His,&Flag Tag (MALS verified)
CDG-M52D2HEK293Mouse CD3 epsilon&CD3 gamma Heterodimer Protein, His Tag&Flag Tag (MALS verified)
CDG-M52W2HEK293Mouse CD3 epsilon&CD3 gamma Heterodimer Protein,Fc,His Tag&Fc,Flag Tag (MALS verified)
CDG-M58D2HEK293Biotinylated Mouse CD3 epsilon&CD3 gamma Heterodimer Protein, His,&Flag Tag (MALS verified)
CDG-R82D4HEK293Biotinylated Rat CD3 epsilon&CD3 gamma Heterodimer Protein, His,&Flag Tag (MALS verified)
CDG-R82D3HEK293Biotinylated Rabbit CD3 epsilon&CD3 gamma Heterodimer Protein, His,&Flag Tag (MALS verified)
CDG-RB52D5HEK293Rabbit CD3 epsilon&CD3 gamma Heterodimer Protein, His Tag&Flag Tag (MALS verified)
MoleculeCat. No.HostProduct DescriptionStructure
CD3 epsilonCDE-M525aHEK293Biotinylated Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a,(Clone: OKT3), Ultra-low endotoxin (MALS verified)
CDE-H5223HEK293Human CD3 epsilon Protein, His Tag (MALS verified)Hot
CDE-H5256HEK293Human CD3 epsilon Protein, Fc,His TagHot
CDE-C5226HEK293Cynomolgus CD3 epsilon Protein, His Tag (MALS verified)Hot
CDE-H8224HEK293Biotinylated Human CD3 epsilon Protein, His Tag, ultra sensitivity (primary amine labeling)
CDE-H82E1HEK293Biotinylated Human CD3 epsilon Protein, His,
CDE-HR2H4HEK293HRP-Human CD3 epsilon Protein, His Tag
CDN-H5253HEK293Human CD3 epsilon Protein, Mouse IgG2a Fc Tag (MALS verified)
CDE-C5254HEK293Cynomolgus CD3 epsilon Protein, Fc,His Tag
CDE-M5256HEK293Mouse CD3 epsilon Protein, Fc Tag
MBS-K026
Human CD3E-coupled Magnetic Beads
MoleculeCat. No.HostProduct DescriptionStructure
CD3 deltaCDD-H5224HEK293Human CD3 delta Protein, His Tag
CDD-C52H8HEK293Cynomolgus CD3 delta Protein, His Tag
MoleculeCat. No.HostProduct DescriptionStructure
CD3 gammaCDA-H5254HEK293Human CD3 gamma / CD3G Protein, Fc Tag (MALS verified)
Questions & Answers
Questions & Answers
Q1: Because the molecular weight of the Fc on the two isoforms of CDD-H5255 (Human CD3 epsilon and CD3 delta heterodimer protein, Fc Tag & Fc Tag) are so similar, how can you confirm that CDD-H5255 is a real heterodimer?

A1: We have specially designed this product to ensure that CD3E and CD3D form a heterodimer which is verified through two different aspects: structure and activity. The structure is verified through SEC-MALS and reducing / non-reducing electrophoresis. On the other hand, activity is verified through binding activity to our CD3 antibody, OKT3. OKT3 only recognizes CD3 epsilon in CD3E&CD3D, therefore confirming that CD-H5255 is a real, active heterodimer.

CD3E&CD3D (Cat. No. CDD-H5255) is 1:1 heterodimer as verified by non-reducing electrophoresis and MALS.
CD3E&CD3D-SEC-MALS
CD3E&CD3D (Cat. No. CDD-H5255) can specifically bind to Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), as verified by ELISA.
CD3E&CD3D-ELISA
Q2: Have you tested the affinity between CDE-H5223 (Human CD3 epsilon Protein, His tag) protein and Anti-CD3 antibody (UCHT1)?

A2: No, we have not tested the affinity of UCHT1 and CD3 epsilon (Cat. No. CDE-H5223) protein by SPR and BLI methods. However, previous literatures have identified that UCHT1 cannot bind to CD3 epsilon resulting in no affinity. Please refer to the corresponding literature to learn more.

>> A conformational epitope expressed upon association of CD3-epsilon with either CD3-delta or CD3-gamma is the main target for recognition by anti-CD3 monoclonal antibodies.

Q3: I found that some CD3 products are listed as offline. What does this mean?

A3: Our offline products are products that have been updated with newer, updated products. Our newer products have been further optimized to improve factors such as purity and activity. For example, some of our offline products include CDE-H8223, CDD-H82W1, and CDD-H52W4. Online versions of these products are CDE-H8224 (MALS verified heterodimer purity> 90%), CDD-H82W6 (MALS-verified heterodimer purity>85%), CDD-H52W1 (MALS-verified heterodimer>85%; better binding activity to OKT3) respectively. If you cannot find the corresponding online product to your offline one, please feel free to contact us.

Q4: Is the CD3 protein lyophilized? Has the protein been functionally tested after lyophilization?

A4: Yes, our CD3 proteins are lyophilized. Functional testing of our proteins is performed before and after lyophilization.

Different lyophilized batches of Human CD3 epsilon Protein, His Tag (MALS verified) (Cat. No. CDE-H5223) have the same biological activity as the product before lyophilization, with high consistency and verified by ELISA.
CD3 epsilon-ELISA
Q5: Does CD3 proteins contain BSA?

A5: No. We use trehalose as a protective agent to exclude animal-derived interferences.

Q6: I want to confirm if the Fc tag in CD3E is human IgG1 or mouse IgG1?

A6: The Fc tag is human IgG1. If the species of the Fc tag is unlisted, it can be assumed that our Fc-tagged proteins carry human IgG Fc-tag.

Q7: When selecting and using Fc-tagged CD3E&CD3D and CD3E&CD3G heterodimeric proteins for establishing antibody binding verification methods, what are some potential issues that should be considered when selecting chromogenic secondary antibodies?

A7: One issue to take into consideration is the cross-reactivity between anti-human IgG Fc antibody / llama IgG Fc tags, and anti-llama IgG Fc antibody / human IgG Fc tag. When using the Fc-tagged CD3 antigen to verify antibody binding activity, careful selection of the chromogenic secondary antibody is critical to avoid cross-reactivity.

For more information, please Ask Us Anything About Our CD3 Products

Ask Us Anything About Our CD3 Products

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