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Your Position: > CD3 > CD3E & CD3D > CDD-H5255

Human CD3 epsilon&CD3 delta Heterodimer Protein, Fc Tag&Fc Tag (MALS verified)

  • 동의어
    CD3E & CD3D,CD3 delta & CD3 epsilon,CD3 delta , epsilon
  • 출처
    Human CD3E&CD3D Heterodimer Protein, Fc Tag&Fc Tag (CDD-H5255) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # P07766-1(CD3E) & P04234-1(CD3D)).
    Predicted N-terminus: Asp 23 (CD3E) & Phe 22 (CD3D)
  • 분자 표징
    Online(Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D)) P07766-1 (CD3E) & P04234-1 (CD3D)

    Human CD3E&CD3D Heterodimer Protein, Fc Tag&Fc Tag is produced by co-expression of CD3E and CD3D, has a calculated MW of 38.3 kDa (CD3E) and 35.8 kDa (CD3D). Subunit CD3E is fused with a human IgG1 Fc tag at the C-terminus and subunit CD3D is fused with a human IgG1 Fc tag at the C-terminus. As a result of glycosylation, the protein migrates as 45-60 kDa under reducing (R) condition, and 90-110 kDa under non-reducing (NR) condition (SDS-PAGE).

  • 내독소
    Less than 1.0 EU per μg by the LAL method.
  • 순도

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • 레시피

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • 재구성

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 보관

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Human CD3E&CD3D Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. CDD-H5255) SDS-PAGE gel

Human CD3E&CD3D Heterodimer Protein, Fc Tag&Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Human CD3E&CD3D Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. ) MALS images

The purity of Human CD3E&CD3D Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. CDD-H5255) is more than 90% and the molecular weight of this protein is around 75-85 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human CD3E&CD3D Heterodimer Protein, Fc Tag&Fc TagHuman CD3E&CD3D Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. CDD-H5255) ELISA bioactivity

Immobilized Human CD3E&CD3D Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. CDD-H5255) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), Ultra-low endotoxin (Cat. No. CDE-M120a) with a linear range of 0.8-6 ng/mL (QC tested).

Human CD3E&CD3D Heterodimer Protein, Fc Tag&Fc TagHuman CD3E&CD3D Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. CDD-H5255) ELISA bioactivity

Immobilized Human CD3E&CD3D Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. CDD-H5255) at 1 μg/mL (100 μL/well) can bind Mouse Anti Human CD3 with a linear range of 0.8-6 ng/mL (Routinely tested).

Human CD3E&CD3D Heterodimer Protein, Fc Tag&Fc TagHuman CD3E&CD3D Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. CDD-H5255) ELISA bioactivity

Immobilized Human CD3E&CD3D Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. CDD-H5255) at 1 μg/mL (100 μL/well) can bind UCHT1 with a linear range of 0.8-3 ng/mL (Routinely tested).

Human CD3E&CD3D Heterodimer Protein, Fc Tag&Fc TagHuman CD3E&CD3D Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. CDD-H5255) ELISA bioactivity

Immobilized Human CD3E&CD3D Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. CDD-H5255) at 1 μg/mL (100 μL/well) can bind Bispecific BCMA×CD3 T cell-engaging Antibody with a linear range of 0.8-6 ng/mL (Routinely tested).

  • 백그라운드
    T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
  • Clinical and Translational Updates

      

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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ETD of in-stock products: 4 business days

가격(KRW) : 525,000

가격(KRW) : 3,810,000

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