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IgG Isotype Controls

IgG Isotype Controls
IgG Isotype Control Introduction

Antibody drugs have become an important field in the development of biomedicine. Isotype control antibodies in antibody drug development are of the same class and subtype as the primary antibody developed against the target, but do not specifically bind to a known or potential target. The purpose of using the isotype control is to confirm that the binding or effect of the primary antibody is specific, excluding non-specific interactions with Fc receptor proteins or with other proteins. Meanwhile, isotype control can also be used as an antibody that competes for binding to Fc receptor proteins, which is related to functional blocking antibody perform the same function, which facilitates effective and reasonable evaluation of the efficacy of antibody drugs. Non-specific background is an important variable that needs to be controlled. Setting the corresponding isotype control antibody to detect non-specific binding can reduce the generation of false positive results and evaluate the possible influencing factors accurately in the drug development process.

ACROBiosystems has developed a series of monoclonal antibodies with Human and Mouse species and different subtypes, which can be used as isotype control antibodies in various applications such as ELISA, western blot, immunofluorescence, immunohistochemistry (IHC) and flow cytometry (FACS), etc.

IgG Isotype Control Product List
MoleculeCat. No.SpeciesProduct DescriptionPreorder/Order
IgG ControlDNP-M2HumanHuman IgG1 Kappa Isotype Control (mAb, carrier free, MALS verified)

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DNP-BM2Biotinylated Human IgG1 Kappa Isotype Control (Biotin, mAb, carrier free, MALS verified)

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DNP-MB273Human IgG1 (N297A) Kappa Isotype Control (mAb, carrier free, MALS verified)

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DNP-M915Human IgG3 Kappa Isotype Control (mAb, carrier free)

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DNP-M3Human IgG4 Kappa Isotype Control (mAb, carrier free, MALS verified)

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DNP-FM2FITC-Labeled Human IgG1 Kappa Isotype Control (FITC, mAb, carrier free)

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DNP-M1MouseMouse IgG1 Kappa Isotype Control (mAb, Carrier free, MALS verified)

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DNP-BM1Biotinylated Mouse IgG1 Isotype Control (Biotin, mAb, carrier free, MALS verified)

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DNP-M1A1Mouse IgG1 kappa Isotype Control

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DNP-BM190Biotinylated Mouse IgG1 kappa Isotype Control

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DNP-FM1FITC-Labeled Mouse IgG1 Isotype Control (FITC, mAb, carrier free)

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DNP-FM1A1FITC-Labeled Mouse IgG1 Isotype Control

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DNP-PM1PE-Labeled Monoclonal Mouse IgG1 Isotype Control (Site-specific conjugation)

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IgG Isotype Control Verification Data

High purity and structural homogeneity verified by SDS-PAGE and SEC-MALS

purity_ACA-H421b

Human IgG1 (N297A) Kappa Isotype Control (mAb) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%. The purity of Human IgG1 (N297A) Kappa Isotype Control (mAb) (Cat. No. DNP-MB273) is more than 90% and the molecular weight of this protein is around 140-160 kDa verified by SEC-MALS.

purity_GCF-H5214

Mouse IgG1 Kappa Isotype Control (mAb) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%. The purity of Mouse IgG1 Kappa Isotype Control (mAb) (Cat. No. DNP-M1) is more than 90% and the molecular weight of this protein is around 130-160 kDa verified by SEC-MALS.

High biological activity verified by ELISA

DNP-M2-E1

Immobilized DNP-Albumin Conjugate at 10 μg/mL (100 μL/well) can bind Human IgG1 Kappa Isotype Control (mAb) (Cat. No. DNP-M2) with a linear range of 1-10 ng/mL (QC tested).

DNP-M3-E1

Immobilized DNP-Albumin Conjugate at 10 μg/mL (100 μL/well) can bind Human IgG4 Kappa Isotype Control (mAb) (Cat. No. DNP-M3) with a linear range of 1-15 ng/mL (QC tested).

DNP-MB273-E1

Immobilized DNP-Albumin Conjugate at 10 μg/mL (100 μL/well) can bind Human IgG1 (N297A) Kappa Isotype Control (mAb) (Cat. No. DNP-MB273) with a linear range of 1-10 ng/mL (QC tested).

DNP-M1-E1

Immobilized DNP-Albumin Conjugate, Bovine at 10 μg/mL (100 μL/well) can bind Mouse IgG1 Kappa Isotype Control (mAb) (Cat. No. DNP-M1) with a linear range of 2-20 ng/mL (QC tested).

FACS verified for use as isotype controls

DNP-BM1-F3

2e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 1 μg/mL of Biotinylated Mouse IgG1 Isotype Control (mAb) (Cat. No. DNP-BM1) and positive control respectively, washed and then followed by PE-SA and analyzed with FACS (Routinely tested).

DNP-FM1-F3

2e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 1 μg/mL of FITC-Labeled Mouse IgG1 Isotype Control (mAb) (Cat. No. DNP-FM1) and positive control respectively, and FITC signal was used to evaluate the binding activity (Routinely tested).

DNP-M1A1-F1

2e5 of anti-CD19 CAR-293 cells were stained with 100ul of 1:50 dilution of 2e5 of anti-CD19 CAR-293 cells were stained with 100ul of 1:50 dilution of Mouse IgG1 kappa Isotype Control (Cat. No. DNP-M1A1) and positive control antibody respectively, washed and then followed by PE anti-mouse IgG1 and analyzed with FACS (QC tested).

DNP-BM190-F1

2e5 of anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) Biotinylated Mouse IgG1 kappa Isotype Control (Cat. No. DNP-BM190) and positive control antibody (Cat. No. FM3-BY54) respectively, washed and then followed by PE-SA and analyzed with FACS (QC tested).

DNP-FM1A1-F1

2e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of FITC-Labeled Mouse IgG1 Isotype Control (Cat. No. DNP-FM1A1) and positive control (Cat. No. FM3-FY45P1) respectively, and FITC signal was used to evaluate the binding activity (QC tested).

DNP-PM1-F1

5e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of PE-Labeled Monoclonal Mouse IgG1 Isotype Control (Cat. No. DNP-PM1) and positive control (Cat. No. FM3-HPY53) respectively, and PE signal was used to evaluate the binding activity (QC tested).

Other Isotype Controls

IgG Fc Proteins——Ideal Isotype Control for IgG Structured Drug Development

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