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Your Position: > CAR-T > DNP > DNP-M1

Monoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control (Carrier free, MALS verified)

  • Source
    Monoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control is a chimeric monoclonal antibody recombinantly expressed from human 293 cells (HEK293), which combines the variable region of a mouse monoclonal antibody with human IgG1 constant domain. The mouse monoclonal antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with DNP.
  • Isotype
    Mouse IgG1/kappa
  • Specificity
    Specifically reacts with DNP (Dinitrophenyl) and DNP conjugated proteins.
  • Application
    This antibody is suitable for use as a non-targeting isotype control in various in vitro and in vivo studies. It can also be used as a negative control in various applications such as ELISA, Western blot, immunofluorescence, immunohistochemistry, immunoprecipitation, and flow cytometry. Each laboratory should determine an optimum working titer for use in its particular application.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

    25 mg or larger size will be supplied as liquid and shipped by dry ice. Please inquire the dry ice shipping cost.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. This product is stable after storage at:
    2. -20°C to -70°C for 12 months in lyophilized state;
    3. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
DNP SDS-PAGE

Monoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
DNP MALS images

The purity of Monoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control(Cat. No. DNP-M1) is more than 90% and the molecular weight of this protein is around 130-160 kDa verified by SEC-MALS.

Bioactivity-Elisa
 DNP ELISA

Immobilized DNP-Albumin Conjugate, Bovine at 10 μg/mL (100 μL/well) can bind Monoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control (Cat. No. DNP-M1) with a linear range of 2-20 ng/mL (QC tested).

  • Background
    A hapten is a small molecule that can elicit an immune response only when conjugated with a large carrier such as a protein. Typical haptens include drugs, urushiol, quinone, steroids, etc. Peptides and non-protein antigens usually need conjugating to a carrier protein (such as BSA (bovine serum albumin) or KLH (keyhole limpet hemocyanin) to become good immunogens). Additionally, haptens should be administered with an adjuvant to ensure a high quality immune response. It is important that the hapten design (preserving greatly the chemical structure and spatial conformation of target compound), selection of the appropriate carrier protein and the conjugation method are key conditions for the desired specificity anti-hapten antibodies. We design anti-hapten antibodies based on the HaptenDB information.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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ETD of in-stock products: 4 business days

가격(KRW) : 240,000

가격(KRW) : 1,050,000

가격(KRW) : 2,250,000

가격(KRW) : Inquire

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