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MHC Class I Complex Custom Service

MHC Class I Complex Custom Service
Background
MHC I (Major histocompatibility complex class I), also known as HLA in humans, is a diverse set of cell surface receptors expressed on all nucleated cells in the body. It plays a crucial role in the adaptive immune system. In vivo, MHC I can bind with antigen peptide fragments of intact proteins. The peptide-MHC complex is recognized by the antigen-specific receptors of T lymphocytes, and upon recognition, activated T cells will contribute to the immune response and does help for clear infection. MHC I-displayed peptidome that allows for highly specific T cell-mediated responses which are vitally important for adaptive immunity. Therefore, research on the deep relationship between peptide-MHC I complex and TCR is increasingly significant for the mechanism investigation and drug development.
To better support MHC-related research, ACROBiosystems relies on their mature protein structure design platform alongside their eukaryotic protein expression technology platform to avoid any prokaryotic refolding process instabilities, incorrect complex conformations, and other issues. Using these platforms, we successfully developed a series of natural MHC complexes with high biological activity in both monomer, tetramer forms with various fluorescent labels available. This includes targets such as NY-ESO-1, WT-1, GP100, GPC, and many others. Furthermore, to meet the diverse needs of our customers, we also offer custom MHC-peptide complex development services using our technology platforms to ensure natural conformation according to your specific needs and applications.
MHC Class I Complex Custom Service

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Service Features

Eukaryotic expression platform for a eukaryotic-based conformation

Professional MHC-peptide complex R&D technology platform

Various types of MHC I and antigens are available

Mature bioactivity verification platform: SPR/ELISA/FACS and more

Co-expression of heterodimer results in a more natural conformation

Monomer/Tetramer forms

FITC/PE/APC/Biotin-labeled are optional

Customized Product List
MHC AlleleTarget
HLA-A*0101 HLA-A*0201 HLA-A*0301 HLA-A*1101 HLA-A*2402  HLA-A*3303 HLA-B*1501 HLA-B*1525 HLA-B*3802 HLA-B*4601 HLA-B*0702 
       ……
NY-ESO-1  GP-100 MSLN AFP WT1 MAGE-A1 HPV HBV HIV EBV PRAME
       ……
Customized Product List

Click to Customize


Recommended Products
MoleculeCat. No.SpeciesProduct DescriptionPreorder/Order
ASSAY DATA

High bioactivity verified by FACS

The activity of PE-Labeled Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQV) Tetramer Protein was higher than that of other competitor.

2e5 of NY-ESO-1 TCR-HEK293 cells (pool) were stained with PE-Labeled  Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQV) tetramer Protein(Fig B)and negative control protein respectively (Fig. A), and competitor-M represented competitor protein (Fig. C). PE signal was used to evaluate the binding activity

High binding affinity verified by SPR

Biotinylated Human HLA-A*02:01 NY-ESO-1

Anti-NY-ESO-1 antibody captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Biotinylated Human HLA-A0201 NY-ESO-1 (SLLMWITQC) Complex Protein (Cat. No. HL1-H82E6) with an affinity constant of 16.9 nM as determined in a SPR assay (Biacore 8K) (QC tested).

Human HLA-A*02:01 NY-ESO-1

Anti-NY-ESO-1 antibody captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human HLA-A0201 NY-ESO-1 (SLLMWITQC) Tetramer Protein (Cat. No. HL1-H52E8) with an affinity constant of 1.35 nM as determined in a SPR assay (Biacore 8K) (QC tested).

Biotinylated Human HLA-C*07:02:01 & B2M & RYR

Biotinylated Human HLA-C*07:02:01 & B2M & RYR ,His,Avitag™&Tag Free (Cat. No. HLR-H52W2) immobilized on SA Chip can bind Human KIR2DL3, Fc Tag (Cat. No. KI3-H5258) with an affinity constant of 2.40 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Biotinylated Human HLA-C*07:02:01 & B2M & RYR

Biotinylated Human HLA-C*07:02:01 & B2M & RYR ,His,Avitag™&Tag Free (Cat. No. HLR-H52W2) immobilized on SA Chip can bind Human KIR2DL2, Fc Tag (Cat. No. KI2-H5255) with an affinity constant of 1.01 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).

High bioactivity verified by ELISA

Biotinylated Human HLA-A*02:01 NY-ESO-1(SLLMWITQC)

Immobilized Biotinylated Human HLA-A*02:01 NY-ESO-1 (SLLMWITQC) Complex Protein (Cat. No. HL1-H82E6) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-NY-ESO-1 Antibody, Human IgG1 with a linear range of 0.1-8 ng/mL (QC tested).

Biotinylated Human HLA-A*02:01 NY-ESO-1 (SLLMWITQV)

Immobilized Biotinylated Human HLA-A*02:01 NY-ESO-1 (SLLMWITQV) Complex Protein (Cat. No. HL1-H82E4) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-NY-ESO-1 Antibody, Human IgG1 with a linear range of 0.2-16 ng/mL (QC tested).

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References

  • 1.Thomas C, Tampé R. MHC I assembly and peptide editing - chaperones, clients, and molecular plasticity in immunity. Curr Opin Immunol. 2021 Jun;70:48-56. doi: 10.1016/j.coi.2021.02.004. Epub 2021 Mar 6. PMID: 33689959.

  • 2.Becar M, Kasi A. Physiology, MHC Class I. 2021 Sep 28. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2022 Jan–. PMID: 32310482.

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