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Your Position: > Protein > LAG-3 > LA3-HP2H4

PE-Labeled Human LAG-3 / CD223 Protein, His TagStar Staining

  • Synonym
    LAG3,CD223,FDC
  • Source
    PE-Labeled Human LAG-3 / CD223 Protein, His TagStar Staining(LA3-HP2H4) is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Leu 450 (Accession # P18627-1).
    Predicted N-terminus: Leu 23
  • Molecular Characterization
    LAG-3 Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 60.7 kDa.

  • Conjugate

    PE

    Excitation Wavelength: 488 nm / 561 nm

    Emission Wavelength: 575 nm

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, 0.2% BSA, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
Bioactivity-FACS
 LAG-3 FACS

Flow cytometric analysis of Daudi cells staining with PE-Labeled Human LAG-3 / CD223 Protein, His TagStar Staining (Cat. No. LA3-HP2H4) at 1:10 dilution (10 μL of the antibody stock solution corresponds to labeling of 5e5 cells in a final volume of 100 µL) , compared with negative control protein. PE signal was used to evaluate the binding activity(QC tested).

  • Background
    Lymphocyte activation gene 3 protein (LAG3) is also known as CD antigen CD223 and protein FDC, which belongs to immunoglobulin (Ig) superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. LAG3 /CD223 involved in lymphocyte activation. LAG3 /CD223 binds to HLA class-II antigens.
  • Clinical and Translational Updates

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